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A kind of food waste degrading compound bacteria and its application

A kitchen waste and compound bacteria technology, applied in the directions of bacteria, microorganisms, microorganisms, etc., can solve the problems of poor biochemical treatment effect of kitchen waste, and achieve the effect of high waste reduction rate and safe and convenient use.

Active Publication Date: 2020-05-19
ZHEJIANG HUAQINGYUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings of the poor biochemical treatment effect of kitchen waste in the prior art, a bacterial strain and its composite microbial preparation capable of mass production and stable performance for efficiently degrading kitchen waste are provided

Method used

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  • A kind of food waste degrading compound bacteria and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Strain screening and identification

[0027] (1) Strain screening: use selective medium to screen strains in food waste reactors;

[0028] (2) Re-screening of bacterial strains: screening of high-efficiency bacteria for the single strains screened;

[0029] (3) Strain enrichment: liquid enrichment culture for high-efficiency strains;

[0030] ⑷Construction of composite strains: combine and test the strains;

[0031] ⑸ Identification of strains in complex bacteria: Molecular biological identification of the strains.

[0032] Wherein, step (1) described strain screening, the present invention provides following method:

[0033] Use a sterile pipette gun to pipette 10.0 mL of the biochemical system mixture of the kitchen waste disposer into a conical flask filled with 90.0 mL of sterile water, and mix well. Then take 1.0mL of the diluted solution in a test tube filled with 9.0mL sterile water (-10 -2 ), then use a sterile pipette to pipette 1.0mL from this test tube i...

Embodiment 2

[0064] Comparison of degradation efficiencies of different formulations of composite bacterial agents and single bacterial agents

[0065] The three screened strains of Bacillus amyloliquefaciens HQY-01, Bacillus oleronius HQY-02 and Bacillus tequilensis HQY-03 were fermented and cultured respectively: inoculated to nutrient cultured for 4 days at 35°C with a shaking frequency of 120r / min.

[0066] The nutrient solution contains the following components (containing per volume of the nutrient solution): peptone 10g / L, sodium chloride 5g / L, yeast extract 10g / L, glucose 10g / L, and the pH is adjusted to 7.

[0067] The number of viable bacteria in the culture solution of Bacillus amyloliquefaciens HQY-01, Bacillus vegetable HQY-02 and Bacillus tequila HQY-03 obtained by fermentation was 2.5*10 7 CFU / mL, 2*10 7 CFU / mL, 3.5*10 7 CFU / mL

[0068] Take 6 portions of 100mL food waste culture solution, and add 1*10 7 The number of CFU was added to Bacillus amyloliquefaciens HQY-01 c...

Embodiment 3

[0078] Embodiment 3 composite bacteria preparation is used for the degradation of kitchen waste:

[0079] 1. Fermentation of compound bacterial preparation

[0080]Fermentation comprises the following steps: inoculate the three strains Bacillus amyloliquefaciens HQY-01, Bacillus oleronius HQY-02 and Bacillus tequilensis HQY-03 into the nutrient cultured for 4 days at 35°C with a shaking frequency of 120r / min.

[0081] The nutrient solution contains the following components (containing per volume of the nutrient solution): peptone 10g / L, sodium chloride 5g / L, yeast extract 10g / L, glucose 10g / L, and the pH is adjusted to 7.

[0082] The number of viable bacteria in the culture solution of the obtained Bacillus amyloliquefaciens HQY-01, Bacillus vegetable HQY-02, and Bacillus tequila HQY-03 is respectively 2.5*10 7 cfu / mL, 2*10 7 cfu / mL, 3.5*10 7 cfu / mL

[0083] According to the ratio of the number of effective viable bacteria of Bacillus amyloliquefaciens HQY-01, Bacillus v...

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Abstract

The invention discloses a composite bacterium for degrading food waste, which consists of Bacillus amyloliquefaciens HQY‑01, Bacillus oleronius HQY‑02 and Bacillus tequilensis HQY‑03 , deposited in China Center for Type Culture Collection, address: China, Wuhan, Wuhan University, date of deposit is February 16, 2017, and deposit numbers are CCTCC NO:M2017040, CCTCC NO:M2017039, CCTCC NO:M2017041. The food waste degrading composite bacteria of the invention can be used to degrade food waste, and has high waste reduction rate and high removal rate of COD, ammonia nitrogen and total nitrogen.

Description

technical field [0001] The invention relates to a biological agent in a kitchen waste treatment process, and in particular provides a kitchen waste degrading composite bacterium and application thereof. Background technique [0002] At present, the treatment methods of food waste in my country are mainly biological methods. Due to the characteristics of high organic matter content and complex components of food waste, ordinary biological treatment methods are not effective. Therefore, study the composition of microorganisms in the biochemical treatment system of food waste, screen out the strains that can degrade food waste efficiently, understand the characteristics of each strain, screen out the strains with high-efficiency degradation ability, and carry out compound cultivation to produce compound bacteria that can efficiently degrade food waste agent, which is of great significance to optimize the treatment. [0003] Composite microbial agents refer to microbial agents ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09B3/00C12R1/07
CPCB09B3/00C12N1/20C12N1/205C12R2001/07
Inventor 朱勤华朱慧强周瑾浩戴军张文涛于通
Owner ZHEJIANG HUAQINGYUAN BIOTECH CO LTD
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