Rapid thin-layer identification method for multiple medicines in common goldenrop granule finished products

Abstract

The invention discloses a rapid thin-layer identification method for multiple medicines in common goldenrop granule finished products. The rapid thin-layer identification method comprises the following steps: S1, preparing a reference medicinal material solution, a reference substance solution and a test solution; S2, carrying out thin-layer chromatography identification; and S3, carrying out methodology verification and finished product detection. According to the method, the same test solution and the same developing solvent can be used for simultaneously identifying the bupleurum chinense (monarch drug), scutellaria baicalensis (minister drug) and liquorice (assistant and guide drug) in the common goldenrop granule finished products on a thin-layer plate, so that the identification timeis shortened, the detection efficiency is improved, the consumption of reagents is reduced, and the detection cost is greatly reduced.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a method for rapid thin-layer identification of multi-drug flavors in finished products of Xiaobupleurum granules. Background technique [0002] Xiaochaihu Granules, derived from the classic prescription Xiaochaihu Decoction recorded in Zhang Zhongjing's "Treatise on Febrile and Miscellaneous Diseases" in the Eastern Han Dynasty, is the ancestor of the reconciliation agent. It has the effects of relieving the exterior and dissipating heat, soothing the liver and harmonizing the stomach. It is mainly used clinically for the treatment of diseases such as exogenous diseases and Shaoyang syndrome of evil criminals. In the prescription, Bupleurum reconciles Shaoyang, permeates external evils, soothes the liver and relieves depression as the monarch drug, Scutellaria baicalensis bitter and cold clears the heat of the liver and gallbladder, helps...

AI Technical Summary

Problems solved by technology

Method (1) In the identification of baicalin, the test solution needs to be subjected to a series of tedious and time-consuming steps such as ultrasonic treatment with ethanol, evaporation to dryness, adjustment of pH value with hydrochloric acid, and extraction with ethyl acetate.

Method (2) In the identification of licorice, the reference medicinal material of licorice needs to be concentrated after decocting for 30 minutes, and the operation is time-consuming; the developing agent is chloroform:methanol:water at 40:10:1, wherein chloroform is the second Precursor reagents, and the amount used is relatively large, which is harmful to the health of operators

Method (3) In the identification of Bupleurum chinensis, the test solution needs to be prepared by self-made polyamide chromatographic column, the operation is complicated, the time is long, the efficiency is low, and the cost is high

[0004] The Chinese patent with the authorized announcement number 1493308A discloses a detection method for Xiaochaihu granules. This method identifies baicalin and glycyrrhetinic acid in Xiaochaihu granules respectively. It is two independent analysis methods, and the detection time is long and thin. The use efficiency of laminates is low and the cost of detection is high; in addition, Bupleurum is the king drug in the prescription, and this method does not identify it, so it cannot comprehensively and effectively control the quality of Xiaochaihu granules

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