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Biodegradation method for zearalenone toxin in DDGS (Distillers Dried Grains with Solubles)

A zearalenone and biodegradation technology, applied in food science and other directions, can solve the problems of harmful chemicals in animal health, loss of nutrients in animal feed, destruction of nutrients, etc. quality effect

Pending Publication Date: 2020-10-27
SHANGHAI HONGYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the method for removing erythralenone in DDGS feed can be divided into chemical method and physical method according to its action principle, among which the physical detoxification method will also cause the loss of animal feed nutrients while removing zearalenone toxin; Although the chemical detoxification method can remove some grain feeds with severe mildew, it is time-consuming and labor-intensive, and it will cause great damage to the nutritional content, and may also produce some chemical substances harmful to animal health.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for the biodegradation of zearalenone toxin in DDGS, comprising the following steps:

[0030] Step 1: Isolation of Bacteria

[0031] 1) In the ultra-clean workbench, shake the collected soil samples in sterile distilled water for 15 minutes to prepare the bacterial suspension, and the shaker speed is 200rpm;

[0032] 2) Dilute the bacterial suspension in a concentration gradient with sterile distilled water and spread it on the NA medium plate, culture it at 30°C for 24 hours, the colony will cover the entire plate, and pick the shape, size and color of the plate with an inoculation loop Strains with different transparency were streaked and purified on plates, and the purified strains were applied to the degradation experiment of erythralenone to obtain Pseudomonas aeruginosa.

[0033] Step 2: Pseudomonas aeruginosa degrades erythralenone

[0034] 1) Pseudomonas aeruginosa was inoculated in liquid NB medium, and after 24 hours of shaking culture at a temperat...

Embodiment 2

[0043] A method for the biodegradation of zearalenone toxin in DDGS, comprising the following steps:

[0044] Step 1: Isolation of Bacteria

[0045] From the fermented sauce after 4 days, the non-toxin-producing filamentous fungi in the sauce grains were qualitatively isolated by using the PDA medium containing β-cyclodextrin, and then the toxin-producing ability of the isolated strains was quantitatively analyzed by ELISA method, and finally Screen out non-toxigenic molds. Re-screen the bacteria that can inhibit the toxin production of Aspergillus flavus, and screen out the strain of Aspergillus niger that can prevent and control mycotoxins.

[0046] Step 2: Degradation of erythralenone by Aspergillus niger

[0047] The isolated strain Aspergillus niger was inoculated into 60ml sterile PDB medium at an inoculum size of 2%, the temperature was set at 28°C, and the shaker was cultivated for 5 days at a shaker speed of 200rpm.

[0048] Step 3: Chromatographic detection

[00...

Embodiment 3

[0053] A method for the biodegradation of zearalenone toxin in DDGS, comprising the following steps:

[0054] Step 1: Isolation of Bacteria

[0055] 1) In the ultra-clean workbench, shake the collected soil samples in sterile distilled water for 15 minutes to prepare the bacterial suspension, and the shaker speed is 200rpm;

[0056] 2) Dilute the bacterial suspension in a concentration gradient with sterile distilled water and spread it on the NA medium plate, culture it at 30°C for 24 hours, the colony will cover the entire plate, and pick the shape, size and color of the plate with an inoculation loop 1. Strains with different transparency were purified by streaking on plates, and the purified strains were applied to the degradation experiment of erythralenone to obtain Pseudomonas aeruginosa;

[0057] 3) The non-toxin-producing filamentous fungi in the fermented sauce were qualitatively isolated from the fermented sauce with β-cyclodextrin-containing PDA medium, and then t...

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Abstract

The invention relates to the technical field of biodegradation, and discloses a biodegradation method for zearalenone toxin in DDGS (Distillers Dried Grains with Solubles). The biodegradation method comprises the following steps of: in a super clean workbench, placing a collected soil sample in sterile distilled water to be oscillated for 15 minutes to prepare a bacterial suspension, keeping the revolving speed of a table concentrator at 200 rpm, after the bacterial suspension is subjected to concentration gradient dilution by the sterile distilled water, coating a NA culture medium flat platewith the bacterial suspension, culturing for 24 hours under a condition of 30 DEG C, enabling a bacterial colony to be spread on the whole flat plate, picking bacterial strains with different forms,sizes, colors and transparency by an inoculating loop to carry out lineation purification, carrying out spot inoculation on the purified the bacterial strains, and applying the purified the bacterialstrains to a zearalenone degradation experiment to obtain pseudomonas aeruginosa. According to a characteristic that the pseudomonas aeruginosa and aspergillus niger can be used for degrading the zearalenone toxin, the pseudomonas aeruginosa and the aspergillus niger are mutually cooperated to efficiently degrade the content of the zearalenone toxin in a DDGS feed product, so that the quality of the DDGS feed product is improved, safety is high, and no pollution is caused so as to be favorable for large-scale utilization.

Description

technical field [0001] The invention relates to the technical field of biodegradation, in particular to a biodegradation method for jaaralenone toxin in DDGS. Background technique [0002] Chiaralenone, also known as F-2 toxin, is a phenolic dihydroxybenzoic acid lactone structure with strong estrogenic activity. The toxin is heat stable and is not destroyed by long-term storage, baking or addition of propionic acid or mold growth inhibitors. [0003] The accumulation of erythralenone in humans and animals can induce a series of symptoms of estrogen effects, including affecting the breast development of female mammals, vulvovaginitis, estrous cycle disorders, pseudopregnancy, miscarriage, stillbirth and teratogenicity, etc. In addition, there are studies It has been confirmed that erythralenone also has genotoxicity, cytotoxicity, immunotoxicity, and tumor-causing toxicity. [0004] Ebralenone is present in a variety of cereal crops worldwide, such as: corn, barley, oats, ...

Claims

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Application Information

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IPC IPC(8): A23L5/20
CPCA23L5/28
Inventor 张玉国张楠楠
Owner SHANGHAI HONGYUAN BIOTECH
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