Micro-fluidic chip for detecting glycosylated hemoglobin and detection method thereof
A glycosylated hemoglobin, microfluidic chip technology, applied in chemical instruments and methods, laboratory containers, biological tests, etc., can solve the problems of complicated operation procedures, high instrument cost, unfavorable diabetes application and promotion, etc., to simplify Operational flow, simple operation, and the effect of shortened sample processing time
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Embodiment 1
[0033] refer to figure 1 and figure 2 As shown, a microfluidic chip for detecting glycosylated hemoglobin provided by the embodiment of the present invention, the microfluidic chip is provided with a cover sheet layer 1 and a fluid layer 2 with a hollow channel structure in sequence from top to bottom and the base layer 3 , the hollow channel structure and the base layer jointly constitute a sample detection unit.
[0034] The cover layer is respectively provided with a sampling through hole 11 for sampling in the sampling chamber and a waste liquid outlet through hole 12 for draining out the waste liquid from the waste liquid pool.
[0035] The sample detection unit includes one or more sample injection chambers 21, a dilution chamber 22, an affinity chromatography channel 23, a detection chamber 24 and a waste liquid pool 25, and the sample injection through hole 11 communicates with In the sampling chamber 21 , the waste liquid outlet through hole 12 communicates with th...
Embodiment 2
[0060] refer to image 3 As shown, a microfluidic chip for detecting glycosylated hemoglobin provided in this example differs from Example 1 in that:
[0061] The sample detection unit includes two sample injection chambers 21 , and the two sample injection chambers 21 are respectively connected to the dilution chamber 22 for introducing different samples into the dilution chamber 22 . When in use, the whole blood sample is introduced from the first sampling chamber 211 , and in step S2 , the diluent is injected from the second sampling chamber 212 and detected according to the detection steps in Example 1.
[0062] The microfluidic chip for detecting glycosylated hemoglobin provided in this embodiment only needs to sequentially inject whole blood, diluent and mobile phase from the sample injection chamber 21 during use, which can realize fully automatic detection, and it can be completed within 3 to 10 minutes. Able to detect glycosylated hemoglobin. This embodiment has the...
Embodiment 3
[0064] refer to Figure 4 As shown, a microfluidic chip for detecting glycosylated hemoglobin is provided in this example, the difference from Example 1 is:
[0065] The microfluidic chip of this embodiment includes three sample detection units. During detection, three different whole blood samples are added to the corresponding sampling chamber 21 respectively, and the detection is carried out according to the detection steps in Example 1, and the detection results of glycated hemoglobin of the three samples can be obtained within 10 minutes. This embodiment has the advantages of convenient sample injection, less sample consumption, high reaction efficiency, and short detection time.
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