Method for rapidly detecting sibutramine in weight-reducing health food

A health food, fast technology, applied in material inspection products, measuring devices, biological tests, etc., can solve the problems of being too time-consuming and unsuitable, and achieve the effect of convenient operation, simple steps, and improved sensitivity

Pending Publication Date: 2020-10-02
UNIV OF ELECTRONICS SCI & TECH OF CHINA ZHONGSHAN INST +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method must use two antibodies to recognize one analyte, which is not suitable for the detection of small molecules such as sibutramine
Some people also think that through continuous dilution, the dilution factor required to reach the detection limit of the method can be tested, and the concentration of the analyte can also be estimated. However, the disadvantage of this method is that it is too time-consuming

Method used

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  • Method for rapidly detecting sibutramine in weight-reducing health food
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  • Method for rapidly detecting sibutramine in weight-reducing health food

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Embodiment Construction

[0022] Attached below Figure 1-5 Embodiments of the present invention will be described in detail.

[0023] 1 Materials and methods

[0024] 1.1 Materials and reagents

[0025] Nitrocellulose membrane (NC membrane), fluorescent silicon ball pad, sample pad, absorbent paper and PVC bottom plate; amino-modified immunofluorescent microspheres (made of polystyrene); sibutramine standard products (sibutramine, N-monodesmethyl sibutramine, N, N-bis demethyl sibutramine, benzyl sibutramine, hamoxibutramine), and other reagents, other reagents are domestic analytical grade . Sibutramine monoclonal antibody and sibutramine antigen were provided by Shenzhen Sanfangyuan Biotechnology Co., Ltd.

[0026] The above-mentioned nitrocellulose membrane (NC membrane), fluorescent silicon ball pad, sample pad, absorbent paper and PVC bottom plate were purchased from Millipore Company in the United States, and the amino-modified fluorescent microspheres were made of pyrogen biological product...

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Abstract

The invention discloses a method for rapidly detecting sibutramine in weight-reducing health food. The method is based on a competitive immunochromatography method. A ternary system is formed by the coating antigen, the antibody and a to-be-detected object, antibody concentration is reduced by gradients, the method sensitivity can be changed, four detection lines coated with antigens are arranged,the reaction concentration of an antibody is reduced in a gradient manner, multi-stage sensitivity immunoreaction is constructed on a single test strip, and a sibutramine competitive immunochromatography semi-quantitative detection method capable of being directly read by naked eyes based on irradiation of a common fluorescent flashlight is constructed in combination with the high sensitivity characteristic of fluorescent microspheres. The method is the first application of a fluorescent microsphere immunochromatography technology in sibutramine detection, is simple and convenient in operation steps, low in economic cost, high in sensitivity and reliable in result, can be used for qualitative and semi-quantitative screening analysis of sibutramine illegal additives in health-care food, and meets the requirement of on-site rapid screening of sibutramine.

Description

【Technical field】 [0001] The invention relates to a detection method for sibutramine, in particular to a method for rapidly detecting sibutramine in weight loss health food. 【Background technique】 [0002] Sibutramine is a synthetic weight loss drug with the chemical formula C 17 h 26 ClN is mainly used in the treatment of obesity in clinical medicine, and suppresses appetite by controlling the nerve center. The main mechanism of action is to inhibit the reuptake of norepinephrine, 5-hydroxytryptamine and dopamine, increase the concentration of norepinephrine, 5-hydroxytryptamine and dopamine in the synaptic space, and then stimulate the hypothalamic satiety center, suppress appetite, and reduce carbohydrates. Absorption of compounds, weight loss. Long-term use of sibutramine can lead to severe mental symptoms such as dizziness, depression, anxiety, and decreased coordination function, and even have the risk of serious cardiovascular diseases such as non-fatal heart attac...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/558
CPCG01N33/94G01N33/582G01N33/585G01N33/558
Inventor 谭贵良张世伟胡敏李向丽李雪雁李梅
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA ZHONGSHAN INST
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