A kind of hemostatic material based on the combination of chitosan and tissue factor and its preparation method

Abstract

A hemostatic material based on a combination of chitosan and tissue factor and a preparation method thereof, aiming at short hemostatic time and good hemostatic effect; the main components of the hemostatic material and their formula in parts by mass are: 100 parts of chitosan, human Recombinant tissue factor 0.1-0.2 milliparts, phospholipids 0.04-0.06 parts, trehalose 0.1-0.2 parts, CaCl 2 0.04-0.08 part; the preparation method is to prepare human recombinant tissue factor and phospholipids into an emulsion under high pressure, and then add 0.02 ml of emulsion per gram of carboxymethyl chitosan to prepare carboxymethyl chitosan and human recombinant tissue Factor mixture; pre-freeze at -40°C to -80°C for 5-10 hours and then vacuum freeze-dry; at an ambient temperature of 15-20°C and a humidity of 30%, grind it through a 100-mesh sieve into powder for external use Rapid hemostasis material.

Description

technical field [0001] The invention relates to a hemostatic composition, which is a rapid hemostatic material for external use. Background technique [0002] Blood is a kind of red opaque viscous liquid flowing in human blood vessels and heart. It has the physiological functions of transportation, defense and regulation of osmotic pressure, acid-base balance and body temperature, and is an important substance for maintaining life. The total blood volume of adults accounts for about 8% of the body weight, which is about 4000-5000 ml. When traumatic bleeding occurs and blood vessels rupture, more than 1 / 3 of the total blood volume will be lost in a short period of time, and the face will appear pale and appear. Cold sweat, drop in blood pressure, weak pulse, etc., and even shock, leading to death. Therefore, rapid hemostasis and coagulation are the guarantee of blood supply to important organs such as the heart, brain, and kidneys, and the guarantee of life. All kinds of tr...

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Problems solved by technology

Among the hemostatic materials commonly used in the market today, such as gelatin, which is non-antigenic and easy to absorb, quickly absorbs water and swells to activate platelets and form blood clots, but there are problems of animal origin and tissue inflammation; zeolite can quickly absorb water and increase the concentration of coagulation factors , to promote blood coagulation, but due to absorbing water and releasing a lot of heat, the local temperature of the wound will exceed 90 degrees Celsius, causing skin burns and affecting wound healing; as a new generation of hemostatic drug, chitosan has good biocompatibility and can be biodegraded. No rejection, its molecules directly gather platelets and red blood cells on the wound surface to promote blood coagulation, but the hemostatic effect of chitosan alone is still limited

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