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Mouse zinc deficiency model feed and preparation method and application thereof

A mouse, zinc deficiency technology, applied in the field of experimental animals, can solve problems such as the inability to use diet to feed mice

Pending Publication Date: 2020-10-02
常州鼠一鼠二生物科技有限公司
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Problems solved by technology

[0003] Aiming at the deficiencies of the above-mentioned prior art, the present invention provides a method for preparing a mouse zinc deficiency model feed, the purpose of which is to solve the problem that the existing mice cannot be fed with a simple diet, and to prepare a feed that is similar to the pathogenesis of human non-alcoholic fatty liver disease. Technical Issues in the Mouse Model of Zinc Deficiency Disease

Method used

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  • Mouse zinc deficiency model feed and preparation method and application thereof
  • Mouse zinc deficiency model feed and preparation method and application thereof
  • Mouse zinc deficiency model feed and preparation method and application thereof

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Embodiment

[0023] The preparation method of the mouse zinc deficiency model feed provided by the present embodiment, the specific technical scheme is as follows:

[0024] The preparation method of mouse zinc deficiency model feed comprises the steps:

[0025] S1, accurately weigh the raw materials shown in Table 1, and mix 20% egg white powder, 15% cornstarch, 50.25% sucrose, 5% cellulose, 35.01% complex minerals, 1.04% complex vitamins and 2 % choline bitartrate is mixed to obtain mixed powder, wherein the zinc content of egg white powder is less than 0.000132%, the zinc content of cornstarch is less than 0.0001%, the zinc content of cellulose is less than 0.0001%, compound minerals and multivitamins The zinc content of all is 0;

[0026] S2, adding 5% corn flour to the mixed powder in step S1 and stirring to obtain an oil mixture;

[0027] S3, adding water to the oil mixture in step S2, stirring and mixing, and granulating through a granulator with a PP material mold to obtain a soft...

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Abstract

The invention belongs to the technical field of experimental animals and particularly relates to a preparation method of mouse zinc deficiency model feed. The method comprises: weighing basal feed accurately and 2% cholesterol for mixing; adding grease with a heat ratio of 40% for stirring; adding moisture for stirring and mixing, and carrying out granulation with a granulation machine to obtain soft particle feed; and carrying out low-temperature moisture drying to obtain the model feed of mouse zinc deficiency. The invention also provides the mouse zinc deficiency model feed and applicationthereof. In the research, through analysis of raw material components and process improvement, the zinc deficiency feed nearly containing no zinc is prepared, and the model feed in current zinc deficiency disease research is solved. A mouse is fed only with the pure feed without use of a gavage method, so a mouse zinc deficiency model is obtained. Animal ethics are satisfied.

Description

technical field [0001] The invention belongs to the technical field of experimental animals, and in particular relates to a mouse zinc deficiency model feed and a preparation method and application thereof. Background technique [0002] Zinc is an important trace element in the body and is widely distributed in the body. The functions of zinc include: participating in the synthesis of various enzymes and hormones, maintaining the structure and function of biofilms and the normal morphology of epithelial cells, and regulating the growth and development of the body. Apoptosis is the autonomous and orderly death of cells controlled by genes to maintain the stability of the internal environment of the body. Studies have shown that zinc deficiency can lead to apoptosis of cells in the body's brain, liver, kidney, testis and immune system. Especially during growth period, zinc deficiency can lead to apoptosis and even atrophy of mouse thymus and testis cells. At present, the zi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/50A23K10/20A23K20/163A23K20/20A23K20/174A23K20/105A23K20/158A23K40/10A01K67/02
CPCA23K50/50A23K10/20A23K20/163A23K20/20A23K20/174A23K20/105A23K20/158A23K40/10A01K67/02
Inventor 姜海善
Owner 常州鼠一鼠二生物科技有限公司
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