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A method for inhibiting algae in water body

A water and algal cell technology, applied in chemical instruments and methods, water/sewage multi-stage treatment, light water/sewage treatment, etc. The effect of exacerbating energy metabolism disturbance and intracellular oxidative stress

Active Publication Date: 2021-06-15
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the bottleneck problem that traditional methods are difficult to effectively control the growth of algae in reclaimed water, ultraviolet algae inhibition has gradually attracted attention
However, the anti-algae effect of ultraviolet rays is positively correlated with the dose. After a period of time, the algae begin to resume growth, and a longer inhibition period requires a higher ultraviolet dose.
Considering the limited space and load capacity of the algae prevention and control device, there are limitations in the way of only increasing the number of UV lamps

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Choose an initial cell density of 4x10 5 Each / mL Microcystis aeruginosa sample, the initial photosynthetic activity of the sample was measured by a modulation fluorometer to be 0.53. Using C-band ultraviolet light with a wavelength of 254nm at 75mJ / cm 2 A dose of 100 mL of sample was irradiated, and immediately after the irradiation was completed, berberine was added to the sample at a dose of 0.2 mg / L.

[0021] After this treatment, the growth inhibition period of Microcystis aeruginosa was extended to 10 days. The samples were measured by the flow cytometer double staining method, and the measured proportion of membrane damaged cells increased to 72% on the seventh day. The photosynthetic activity of treated M. aeruginosa decreased by 53% immediately after treatment and did not return to the initial level until 9 days later.

Embodiment 2

[0023] Choose an initial cell density of 4x10 5 Each / mL Microcystis aeruginosa sample, the initial photosynthetic activity of the sample was measured by a modulation fluorometer to be 0.53. Using C-band ultraviolet light with a wavelength of 254nm at 75mJ / cm 2 A dose of 100 mL of sample was irradiated, and immediately after the irradiation was completed, berberine was added to the sample at a dose of 0.5 mg / L.

[0024] After this treatment, the growth inhibition period of Microcystis aeruginosa was extended to 12 days. The sample was measured by the flow cytometer double staining method, and the measured ratio of membrane damaged cells increased to 86% on the seventh day. The photosynthetic activity of treated M. aeruginosa decreased by 51% immediately after treatment and did not return to the initial level until 12 days later.

Embodiment 3

[0026] Choose an initial cell density of 4x10 5 Each / mL Microcystis aeruginosa sample, the initial photosynthetic activity of the sample was measured by a modulation fluorometer to be 0.53. Using C-band ultraviolet light with a wavelength of 254nm at 75mJ / cm 2 A dose of 100 mL of sample was irradiated, and immediately after the irradiation was completed, berberine was added to the sample at a dose of 1 mg / L.

[0027] After this treatment, the growth inhibition period of Microcystis aeruginosa was extended to 21 days. The samples were measured by the flow cytometer double staining method, and the measured ratio of membrane damaged cells increased to 91% on the seventh day. The photosynthetic activity of treated M. aeruginosa decreased by 40% immediately after treatment and did not return to the initial level until 18 days later.

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Abstract

The invention discloses a method for inhibiting algae in a water body, which is carried out sequentially according to the following steps: (1) irradiating the water body with ultraviolet light; (2) adding allelochemical substances to the water body. Compared with the prior art, the water algae inhibition method provided by the present invention can effectively inhibit the growth of algae in the water body, achieve an inhibition period of more than 2-3 weeks, produce faster algal cell lethality, and effectively control microcystins content, interfere with its intracellular synthesis process, and significantly reduce the dosage of ultraviolet rays and allelochemicals, which helps to prevent and control the practical application of algae devices in landscape water bodies, and effectively prevent and control the outbreak of algae blooms in landscape water bodies.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, in particular to a method for inhibiting algae in water bodies. Background technique [0002] The shortage of water resources is increasing worldwide. Reclaimed water has become an important source of water supply for water-scarce urban landscape water bodies. However, reclaimed water still contains high concentrations of nutrients such as nitrogen and phosphorus. There are receiving water bodies in the process of landscape environment utilization. Risk of eutrophication and algal blooms. In view of the bottleneck problem that traditional methods are difficult to effectively control the growth of algae in reclaimed water, ultraviolet algae inhibition has gradually attracted attention. However, the anti-algae effect of ultraviolet rays is positively correlated with the dose. After a period of time, the algae begin to resume growth, and a longer inhibition period requires a higher...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F9/08
CPCC02F1/32C02F1/50
Inventor 胡洪营李尚陶益朱小琴
Owner TSINGHUA UNIV
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