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A kind of Lactobacillus paracasei lc-37 with promoting digestion and its application

A technology of LC-37 and Lactobacillus, which is applied in the direction of Lactobacillus, cheese substitutes, bacteria used in food preparation, etc., can solve the problems of lack of effective evaluation and lack of promotion of digestion, and achieve improvement of indigestion, abdominal pain, increase The effect of content

Active Publication Date: 2022-06-28
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are few studies on the digestion-promoting effect of Lactobacillus paracasei, and the probiotics used in the development of lactic acid bacteria beverage products mainly rely on imports, lack of strains with our independent intellectual property rights, and lack of effective evaluation of functions related to promoting digestion

Method used

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  • A kind of Lactobacillus paracasei lc-37 with promoting digestion and its application
  • A kind of Lactobacillus paracasei lc-37 with promoting digestion and its application
  • A kind of Lactobacillus paracasei lc-37 with promoting digestion and its application

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Effect test

Embodiment 1

[0046] The acquisition of embodiment 1 Lactobacillus paracasei strain LC-37

[0047] 1. Isolation of Lactobacillus paracasei strain LC-37

[0048] The intestinal contents of the healthy and long-lived elderly people in Guangxi Bama were collected, that is, feces, and the intestinal feces samples of the healthy and long-lived elderly people in Guangxi Bama were serially diluted with diluent. Different morphological colonies, microscopically select Gram-positive bacilli, and continue to use the pour plate method to purify the strain until the pure culture of the strain is isolated. After the above separation process, a Lactobacillus strain was obtained.

[0049] The preparation method of the diluent used in the above dilution step: 4.5 g of sodium dihydrogen phosphate, 6.0 g of disodium hydrogen phosphate, 0.5 g of L-cysteine, 0.5 g of agar, 0.5 mL of Tween 80, 1000 mL of distilled water, Heat to dissolve, adjust pH to 7.4-7.6, sterilize at 121°C for 15min, and set aside.

[...

Embodiment 2

[0053] Embodiment 2 Lactobacillus paracasei LC-37 bacteria powder

[0054] The present embodiment provides a preparation method of Lactobacillus paracasei LC-37 bacterial powder, which specifically includes the following steps:

[0055] 1. Expanded culture of strains

[0056] The Lactobacillus paracasei LC-37 cryopreserved strain was inoculated into 10 mL of MRS medium, and placed at 37 °C for 12 hours to obtain a seed culture solution suitable for inoculation. bacteria.

[0057] 2. Fermentation

[0058] The seed culture liquid obtained above was inoculated into the fermentation medium at an inoculum amount of 2% to 5% (v / v) (2% v / v was selected in this example). In this example, the fermentation medium was: The MRS medium was cultured at 33°C with a stirring speed of 200 rpm, the pH of the medium was kept at pH 5.0, and the fermentation was carried out for 20 hours. After the fermentation, the culture solution was centrifuged by using a disc centrifuge at a rotational spe...

Embodiment 3

[0061] Embodiment 3 Lactobacillus paracasei LC-37 bacterial powder

[0062] The present embodiment provides a preparation method of Lactobacillus paracasei LC-37 bacterial powder, which specifically includes the following steps:

[0063] 1. Expanded culture of strains

[0064] The Lactobacillus paracasei LC-37 cryopreserved strain was inoculated into 10 mL of MRS medium, and placed at 37 °C for 12 hours to obtain a seed culture solution suitable for inoculation. bacteria.

[0065] 2. Fermentation

[0066] The seed culture liquid obtained above was inoculated into the fermentation medium at an inoculum amount of 2% to 5% (v / v) (5% v / v was selected in this example). In this example, the fermentation medium was: The MRS medium was cultured at 40° C., the stirring speed was 100 rpm, the pH of the medium was maintained at pH 6.0, and the fermentation was carried out for 14 hours. After the fermentation, the culture solution was centrifuged with a disc centrifuge at a rotational...

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Abstract

The invention belongs to the field of microbial fermentation, and specifically relates to a kind of Lactobacillus paracasei (Lactobacillus paracasei) LC-37 with a function of promoting digestion and its application. A kind of Lactobacillus paracasei (Lactobacillus paracasei) LC-37 provided has been preserved in China The General Microbiology Center of the Microbiological Culture Collection Management Committee, its deposit number is CGMCC NO.14055. Described Lactobacillus paracasei LC-37 is isolated from the intestinal contents of healthy and long-lived elderly people in Bama, Guangxi, and described Lactobacillus paracasei LC-37 has the effect of promoting digestion, and is specifically manifested as abdominal pain, belching, Improvement of indigestion symptoms such as pantothenic acid, bloating, loss of appetite, diarrhea or constipation, while increasing the content of short-chain fatty acids in the intestine.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to Lactobacillus paracasei LC-37 with the effect of promoting digestion and application thereof. Background technique [0002] Lactobacillus paracasei is one of the Lactobacillus species, and is an important probiotic that is often used in the production of dairy products, health food, beverages and ice cream. Numerous studies have shown that Lactobacillus paracasei has the functions of regulating intestinal flora and enhancing immunity. Such as a kind of Lactobacillus paracasei ET-22 with the function of relieving constipation disclosed in Chinese patent document CN110892989A, which introduces a kind of Lactobacillus paracasei ET-22 with the function of relieving constipation, the main function of this strain is to improve the Intestinal propulsion rate, shortening the time of intestinal operation, thus playing a role in relieving constipation. Another example is...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P1/14A21D2/08A23C9/123A23L11/50A23G3/36A23G9/36A23L2/02A23L2/39A23L33/135
CPCC12N1/20A61K35/747A61P1/14A23L33/135A23L2/39A23C20/025A23L2/02A23C9/1234A23G9/363A23G3/366A21D2/08A23V2002/00A23V2400/165A23V2250/5114A23V2250/28A23V2250/5118A23V2200/242A23V2250/156A23V2250/70A23V2200/3204A23V2300/20A23V2250/636Y02A50/30
Inventor 孙二娜孙健牛天娇赵伊凡赵红峰李金玉白洋郭永杰姚斐邓凤生刘巨龙江雷王辰元肖然
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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