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Microemulsion-based vaccine delivery system, preparation method and application thereof

A delivery system and microemulsion technology, applied in the field of medicine, can solve problems such as adverse reactions, achieve good dispersion, simple operation, and easy clinical use

Active Publication Date: 2020-09-15
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although emulsions such as Freund's incomplete adjuvant have also been used in humans, including in influenza virus vaccines, they are prone to adverse reactions

Method used

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  • Microemulsion-based vaccine delivery system, preparation method and application thereof
  • Microemulsion-based vaccine delivery system, preparation method and application thereof
  • Microemulsion-based vaccine delivery system, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] The preparation method of aluminum hydroxide microemulsion: dissolving 0.2g Span 85 in 0.5g squalene, dissolving 0.2g Tween 80 in water for injection, mixing to form colostrum, which is homogenized under high pressure to form microemulsion 40ml, take 1ml of microemulsion, add 5mg DSPE-PEG powder to it, stir to dissolve it, add 312.5μl 37.5mM aluminum sulfate aqueous solution, add 1.75ml 100mM HEPES buffer solution with pH 8 under vortex to adjust to neutrality, and obtain .

Embodiment 2

[0084] The preparation method of aluminum hydroxide microemulsion: 0.35g egg yolk lecithin and 0.01g Tween 80 are dissolved in water for injection, mixed with 0.5g squalene to form colostrum, and the colostrum is homogenized under high pressure to form 40ml microemulsion. Take 1ml of the above emulsion, add 123μl of 37.5mM aluminum sulfate aqueous solution, and add 615μl of 100mM HEPES buffer solution with pH 8 under vortex to adjust to neutrality.

Embodiment 3

[0086] The preparation method of aluminum hydroxide microemulsion: dissolve 0.1g oleic acid and 0.2g Span 85 in 0.5g squalene, dissolve 0.2g Tween 80 in water for injection, mix to form colostrum, and the colostrum is subjected to high pressure Homogenize to form microemulsion 40ml. Take 1ml of the above emulsion, add 100μl 37.5mM aluminum sulfate aqueous solution, and add 550μl 100mM HEPES buffer solution with pH 8 under vortex to adjust to neutrality.

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Abstract

The invention provides a microemulsion-based vaccine delivery system, and also provides a preparation method and application thereof. According to microemulsion in the invention, a series of metal ioncompounds are adsorbed; and antigens are added in a preparation process, so that antigen entrapment can be realized and a stable vaccine preparation is obtained. The vaccine prepared by the inventioncan be efficiently taken in by antigen presenting cells, effectively transferred to lymph nodes and induce antigen-specific immune response, and has a wide application prospect.

Description

technical field [0001] The invention relates to a microemulsion-based vaccine delivery system and a preparation method thereof, belonging to the technical field of medicine. Background technique [0002] Vaccination is one of the greatest inventions of modern medicine and an effective means of prevention and eradication of many diseases, used to control many infectious diseases. The three important directions of vaccine development include: the discovery of new antigens, new adjuvant technology and new carrier platform. [0003] Traditional live attenuated and inactivated vaccines may contain natural adjuvant components in nature, which may include proteins, lipids and oligonucleotides in particulate form. However, due to some limitations of these attenuated and inactivated vaccines, such as the mutation of attenuated pathogenic microorganisms into strong pathogenic microorganisms, the inactivated vaccines are not completely inactivated in the preparation, and when these va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K47/02A61K39/00A61K39/39A61P35/00A61P37/02
CPCA61K9/1075A61K47/02A61K39/00A61K39/001186A61K39/39A61P35/00A61P37/02A61K2039/55561A61K2039/575A61K39/001192Y02A50/30A61K2039/55505A61K2039/55566A61K39/0011A61K39/02A61K39/12
Inventor 孙逊陈正军龚涛张志荣
Owner SICHUAN UNIV
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