Sitting drop type cell sphere culture chip and use method thereof

Abstract

The invention provides a sitting drop type cell sphere culture chip and a use method thereof. The sitting drop type cell sphere culture chip comprises a sample adding cavity and a micro-cavity array,wherein the micro-cavity array is arranged at the bottom of the sample adding cavity, super-hydrophobic surfaces are arranged on the surfaces of the sample adding cavity and the micro-cavity array, the micro-cavity array is provided with a plurality of micro-cavities, at least one through hole is formed in each micro-cavity, and the section of each through hole is smaller than that of the corresponding micro-cavity. The invention further discloses the using method of the sitting drop type cell sphere culture chip. According to the invention, the micro-cavity array is combined with the super-hydrophobic surface; the synergistic effect of the super-hydrophobic surface hydrophobic effect, the liquid sample surface tension and the self-gravity is used, automatic distribution of cell suspensionliquid, simple and rapid liquid change in the culture process and simple and rapid recovery of cultured cell spheres can be realized, and the problems of difficult culture medium updating, poor air permeability, poor cell sphere uniformity, difficult in-situ observation, high cost, difficult cell sphere taking out and the like in the prior art are effectively solved.

Description

technical field [0001] The invention belongs to the technical field of cell biology, and in particular relates to a sitting drop type cell sphere culture chip and a using method thereof. Background technique [0002] Compared with the traditional monolayer cell culture technology, the cell spheres formed by three-dimensional cell culture can better reflect the tight and rich three-dimensional connection between cells and between cells and the external microenvironment, and better simulate the natural physiological environment. Cell-intrinsic metabolism (such as nutrients, oxygen, metabolites, etc.) and proliferation gradients, which can track the diffusion path of drugs, are ideal models for cell research. Due to its unique advantages, in recent years, three-dimensional cell sphere culture has been widely used in cell biology and medical research, and has become an indispensable and powerful tool for drug screening, tumor treatment mechanism analysis, stem cell differentiati...

AI Technical Summary

Problems solved by technology

[0003] Aiming at the above-mentioned problems existing in the prior art, the present invention provides a sitting drop cell spheroid culture chip and its use method, which uses a microcavity array combined with a super-hydrophobic surface, through the hydrophobic effect of the super-hydrophobic surface, the surface tension of the liquid sample and its own The synergistic effect of gravity can realize the automatic distribution of cell suspension, the simple and fast medium replacement during the culture process, and the simple and fast recovery of cultured cell spheroids, which effectively solves the difficulties in medium renewal, poor air permeability, and poor uniformity of cell spheroids in the prior art. Difficult to observe in situ, high cost and difficult to remove cell spheroids

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