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A kind of Taizishen active six-carbon aldehyde oligosaccharide and its preparation method and application

A technology of Taizishen and six-carbon aldehyde, applied in the direction of organic active ingredients, pharmaceutical formulations, cosmetic preparations, etc., can solve the problem that there is no active six-carbon aldehyde oligosaccharide of Taizi ginseng, and achieve a reduction in the level of inflammation-related indicators. Effect

Active Publication Date: 2022-04-19
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on active six-carbon aldehyde oligosaccharides from Radix Pseudostellariae

Method used

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  • A kind of Taizishen active six-carbon aldehyde oligosaccharide and its preparation method and application
  • A kind of Taizishen active six-carbon aldehyde oligosaccharide and its preparation method and application
  • A kind of Taizishen active six-carbon aldehyde oligosaccharide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The extraction of embodiment 1 Radix Pseudostellaria active oligosaccharides

[0069] (1) Take 300 grams of dried Pseudostellariae Radix Pseudostellariae (Guangzhou Zhixin Pharmaceutical Co., Ltd.) and place it in a 4L round-bottomed flask, add 2.4L of 70% ethanol solution by volume and extract three times at 80°C for 1 hour each time. After the extraction, remove the ethanol from the medicinal dregs, add 2.4L deionized water to extract three times at 80°C, each time for 1h, combine the three water extracts, concentrate and spray dry to obtain the water extract of Pseudostellaria Pseudostellaria, with a yield of 31.20±3.89 %.

[0070] (2) Add the above-mentioned Pseudostellariae Pseudostellariae extract into water to make the concentration 1g / 100mL, after ultrasonic dissolution, slowly add absolute ethanol, stir while adding so that the final alcohol concentration is 90% (volume percentage), 4 ° C refrigerator Precipitate in alcohol for 24 hours, centrifuge the solutio...

Embodiment 2

[0073] The structural characterization of the active oligosaccharides of embodiment 2 Radix Pseudostellariae Radix

[0074] (1) Determination of monosaccharide composition and molecular weight: Add 0.5 mL of 4 mol / L trifluoroacetic acid to the active oligosaccharides of Radix Pseudostellariae obtained in Example 1, and make it completely acid-hydrolyzed in an oil bath at 120°C for 2 hours, completely acid-hydrolyzed Add 0.5ml of concentrated ammonia water (mass concentration 25%) to the final sample, shake to make the content dissolve completely, then add 0.5ml of PMP (1-phenyl-3-methyl-5-pyrazole) with a concentration of 0.5mol / L Linone) methanol solution, after carrying out PMP derivatization, through liquid mass analysis; Wherein, liquid mass analysis (UPLC / Q-TOFMS analysis) condition is as follows:

[0075] Mobile phase: aqueous phase (A): 0.05% (v / v) acetic acid, 20 mM ammonium acetate; organic phase (B): acetonitrile.

[0076] Chromatographic column: CORTECS C18 PFP col...

Embodiment 3

[0091] Example 3 Effect of Pseudostellariae Pseudostellariae Active Polysaccharides on LPS-Induced RAW264.7 Cell Inflammation Model

[0092] (1) Cell culture Take out the RAW264.7 cell line (Shanghai Cell Bank, Chinese Academy of Sciences) frozen in liquid nitrogen, quickly place it in a 37°C water bath and shake it until the cells melt, then transfer it to a centrifuge tube that has been added to the culture medium, and set the temperature at 1000rpm Centrifuge for 5min, discard the supernatant, add a small amount of culture medium and gently blow and suck evenly, the culture medium is to contain 10% (v / v) fetal bovine serum, 1% (v / v) double antibody (the final concentration of penicillin is 100μg / mL, streptomycin is 50μg / mL) of 1640 medium, the cell suspension was transferred to the culture dish, at 37 ℃, 5% (v / v) CO 2 , Cultivated under saturated humidity conditions;

[0093] (2) Cultivate RAW264.7 cells into a single cell suspension with 1640 medium containing 10% (v / v) F...

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Abstract

The invention discloses an active six-carbon aldehyde oligosaccharide of Radix Pseudostellariae and its preparation method and application. The structure of the active six-carbon oligosaccharide of Radix Pseudostellariae is shown in formula I, the molecular weight is 1476.6185Da, and its monosaccharide composition is: mannose, glucose and galactose (molar ratio is 0.076:0.814:2.600). The present invention also provides a preparation method of the oligosaccharides, through alcohol extraction, water extraction, alcohol precipitation, enzyme-Sevage method for protein removal, and DEAE-52 anion exchange column separation and purification methods to collect products with a molecular weight between 1000Da and 2000Da , to obtain the active six-carbon aldehyde oligosaccharide of Radix Pseudostellariae. The oligosaccharide obtained in the present invention has anti-inflammation, anti-oxidation activity, and strong reducing ability, and can significantly antagonize inflammatory bowel disease by regulating the type and abundance of intestinal flora.

Description

technical field [0001] The invention belongs to the technical field of natural substance extraction, and in particular relates to an active six-carbon aldehyde oligosaccharide from Radix Pseudostellariae and its preparation method and application. Background technique [0002] Radix pseudostellariae (RP) is one of the commonly used traditional Chinese medicines recorded in the Chinese Pharmacopoeia. It is also known as Pseudostellaria heterophylla (Miq.) Pax ex Pax et Hoffm. Pseudostellariae Radix Pseudostellariae has the effect of "invigorating qi and invigorating the spleen, promoting body fluid and moistening the lungs". It is often used clinically for symptoms such as spleen deficiency, fatigue, loss of appetite, weakness after illness, deficiency of qi and yin, spontaneous sweating and thirst, dryness of the lungs and dry cough, etc. It is often used as a strong nourishing Taste. Its nature is flat, slightly bitter and sweet in taste; it returns to the spleen and lung ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61Q19/08A61Q19/02A61P39/06A61P35/00A61P29/00A61P1/10A61P1/00A61K36/36A61K31/715A61K8/73
CPCC08B37/006C08B37/0003A61K31/715A61K36/36A61P29/00A61P39/06A61P1/00A61P1/10A61P35/00A61K8/73A61Q19/08A61Q19/02A61K2800/805
Inventor 芮雯陈宏远游思远陈鸿策
Owner GUANGDONG PHARMA UNIV
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