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Application of UGT3A2 gene and protein coded by UGT3A2 gene in auxiliary diagnosis of upper gastrointestinal tumors

A digestive tract tumor and gene technology, applied in the field of medicine and biology, can solve the problem of less molecular indicators, and achieve the effects of inducing apoptosis, promoting proliferation, and inhibiting tumor cell apoptosis.

Active Publication Date: 2020-08-28
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are very few molecular indicators related to the upper gastrointestinal tract discovered so far, and even fewer are clinically accepted and applied. The auxiliary diagnosis of molecular indicators for patients with upper gastrointestinal tumors is still in the preliminary exploration stage

Method used

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  • Application of UGT3A2 gene and protein coded by UGT3A2 gene in auxiliary diagnosis of upper gastrointestinal tumors
  • Application of UGT3A2 gene and protein coded by UGT3A2 gene in auxiliary diagnosis of upper gastrointestinal tumors
  • Application of UGT3A2 gene and protein coded by UGT3A2 gene in auxiliary diagnosis of upper gastrointestinal tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: UGT3A2 mRNA expression in esophageal squamous cell carcinoma tissue and its paired adjacent normal tissues

[0063] 1. Sample collection:

[0064] Collected 30 cases of esophageal squamous cell carcinoma patients with cancer tissue and their paired adjacent normal tissue samples. All samples were fresh specimens from esophageal squamous cell carcinoma after surgery. None of the patients received radiotherapy or chemotherapy before surgery. Surgery is the preferred treatment option. Quickly freeze in liquid nitrogen, then transfer to -80℃ ultra-low temperature refrigerator for freezing. The esophageal squamous cell carcinoma tissue used in the present invention and its paired adjacent normal tissue samples are all from the State Key Laboratory of Esophageal Cancer Prevention and Treatment jointly established by Zhengzhou University. According to the system stipulated by the ethics review committee, each patient signed an informed consent form before sampling.

[00...

Embodiment 2

[0092] Example 2: Study on the expression of UGT3A2 protein in esophageal squamous cell carcinoma tissue and its paired adjacent normal tissues

[0093] 1. Sample collection:

[0094] Collected 150 cases of esophageal squamous cell carcinoma patients' cancer tissues and their paired adjacent normal tissue samples. All samples were from paraffin tissue samples after esophageal squamous cell carcinoma surgery. None of the patients received radiotherapy or chemotherapy before surgery. Surgery is the preferred treatment option. The esophageal squamous cell carcinoma and its paired adjacent normal tissue samples used in the present invention are all from the State Key Laboratory of Esophageal Cancer Prevention and Treatment jointly established by Zhengzhou University. According to the system stipulated by the ethics review committee, each patient signed an informed consent form before sampling.

[0095] Immunohistochemical method was used to detect the expression level of UGT3A2 protein ...

Embodiment 3

[0108] Example 3: Detection of UGT3A2 protein content in serum and evaluation of its diagnostic value for esophageal squamous cell carcinoma

[0109] 1. Sample collection:

[0110] Serum samples from 60 esophageal squamous cell carcinoma patients (esophageal squamous cell carcinoma group) and 40 normal people (normal control group) from the State Key Laboratory of Esophageal Cancer Prevention and Treatment jointly established by Zhengzhou University were collected and stored at -80°C. Among them, the serum of patients with esophageal squamous cell carcinoma was collected when the patient was initially diagnosed with esophageal squamous cell carcinoma and did not receive any radiotherapy or chemotherapy. Normal human serum was the serum of healthy people who were diagnosed as having no upper digestive tumor by gastroscopy and biopsy. .

[0111] 2. Experimental method

[0112] Enzyme-linked immunosorbent assay was used to detect the content of UGT3A2 protein in serum samples of esophag...

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Abstract

The invention belongs to the technical field of medical biology, and discloses an upper gastrointestinal tumor marker-UGT3A2 gene and protein coded by the same. The expression of the UGT3A2 gene in the tissues of the esophagus cancer, the cardia cancer and the gastric cancer is detected by using RT-PCR and immunohistochemistry technologies, and is compared with that in the paired paracancerous normal tissues, so that the expression of the UGT3A2 gene in the cancer tissues is obviously higher than that in the paired paracancerous normal tissues. The UGT3A2 protein level in serum is detected byutilizing an ELISA technology, and compared with normal people, the UGT3A2 protein content of patients suffering from upper gastrointestinal tumors (the esophagus cancer, the cardia cancer and the gastric cancer) is obviously higher than that of the normal people. Therefore, by detecting the expression level of a UGT3A2 gene expression product in a tissue sample, auxiliary diagnosis can be carriedout on the upper gastrointestinal tumors (the esophageal cancer, the cardiac cancer and the gastric cancer), and a reference basis is provided for diagnosis of clinicians on the gastrointestinal tumors.

Description

Technical field [0001] The invention belongs to the field of medical biotechnology, and specifically relates to the application of UGT3A2 gene and its encoded protein in the auxiliary diagnosis of upper gastrointestinal tumors. Background technique [0002] Upper gastrointestinal tumors are not only high-incidence tumors in the world, but also in my country. Esophageal cancer, gastric cardia cancer, and gastric cancer are the most common malignant tumors of the upper gastrointestinal tract. The global incidence of esophageal cancer and gastric cancer ranks seventh and fifth respectively. Tumor-related mortality was ranked sixth and third respectively. In my country, the incidence and mortality of esophageal cancer and gastric cancer are in the forefront of malignant tumors. Since the 1980s, the incidence of cardia adenocarcinoma has shown an obvious upward trend worldwide. my country is one of the countries with high incidence and high mortality of cardia adenocarcinoma. Early ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574G01N33/573A61K45/00A61P35/00C12N15/113C12N15/867C12N15/90C12N5/10C12R1/91
CPCA61K45/00A61P35/00C12N5/0693C12N15/113C12N15/86C12N15/907C12N2510/00C12N2740/15043C12N2800/107C12Q1/6886C12Q2600/158C12N2310/20G01N33/573G01N33/57407G01N33/57446G01N33/57488G01N2333/91102
Inventor 宋昕靳艳王立东赵学科王盼盼杨媛啧范宗民韩雪娜王苒李贝秦艳茹李秀敏陈志国
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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