A kind of artificial propagation method of spotted carp
A technology of artificial breeding and gnatfish, which is applied to the preservation of human or animal bodies, fish farming, climate change adaptation, etc., can solve the problems of artificial breeding of fish fry, and achieve the effect of solving the difficulty of artificial breeding
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[0030] Preparation of antifreeze
[0031] (A) Preparation of the premix: first add 0.2-0.3kg of evening primrose oil dropwise to 1kg of egg white while stirring, stir and mix well, then heat in a water bath at 55-65°C for 5-8 minutes, 10000-12000r / min stirring for 15-20 minutes, centrifuging to take the supernatant; then adding 0.05-0.08kg papaya seed stem cell extract and 0.02-0.04kg yeast dextran to the supernatant, and ultrasonically oscillating evenly to obtain the premixed solution. spare;
[0032] (B) Preparation of antifreeze: first mix 3-4 kg of formaldehyde solution with mass concentration of 8-12% with 0.08-0.12 kg of sodium chloride and 1 kg of maltose, then add 0.2-0.3 kg of premixed solution, and ultrasonically oscillate evenly to obtain The antifreeze.
[0033] Reference Example 2
[0034] Preparation of antifreeze
[0035](A) Preparation of the premix: first add 0.2-0.3kg of evening primrose oil dropwise to 1kg of egg white while stirring, stir and mix well...
Embodiment 1
[0054] A kind of artificial propagation method of spotted carp, concrete steps are as follows:
[0055] 1) Preservation of sperm: In April 2019, at the Aquaculture Technology Extension Station of the Agricultural and Rural Bureau of Xingbin District, Laibin City, Guangxi, on April 15, 2 male fish of about 1.5kg were purchased from the market. Bring the testes of the male fish they slaughtered back to the aseptic laboratory of the extension station. After the male fish is slaughtered, take out the testes, wipe off the surface moisture of the testes with a dry towel, and cut the testes into pieces with scissors. The milky white semen can be seen with the naked eye, and then separate Grind it with a homogenizer several times, suck a few drops of semen with a straw and put it on a glass slide, activate it with water and observe the motility of the sperm under a microscope, and divide the semen with a motility greater than 90% into the prepared beakers , add the antifreeze solution...
Embodiment 2
[0062] 1) Preservation of sperm: In April 2019, at the Aquaculture Technology Extension Station of the Agricultural and Rural Bureau of Xingbin District, Laibin City, Guangxi, on April 15, 2 male fish of about 1.5kg were purchased from the market. Bring the testes of the male fish they slaughtered back to the aseptic laboratory of the extension station. After the male fish is slaughtered, take out the testes, wipe off the surface moisture of the testes with a dry towel, and cut the testes into pieces with scissors. The milky white semen can be seen with the naked eye, and then separate Grind it with a homogenizer several times, suck a few drops of semen with a straw and put it on a glass slide, activate it with water and observe the motility of the sperm under a microscope, and divide the semen with a motility greater than 90% into the prepared beakers , add the antifreeze solution obtained in Reference Example 2; the mixed solution containing semen and antifreeze solution is d...
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