Graphene oxide drug carrier as well as preparation method and application thereof

A graphene and drug technology, applied in the field of graphene oxide drug carrier and preparation thereof, can solve problems such as lack of response ability, and achieve the effects of high carrying capacity, safe anti-tumor effect, and avoidance of drug release effect

Active Publication Date: 2020-08-21
南方医科大学口腔医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned PTX@GO-PEG nanoparticles lack the ability to respond to external pH stimuli. How to optimize the nano-drug and customize drug release to form precise treatment is another problem at present.

Method used

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  • Graphene oxide drug carrier as well as preparation method and application thereof
  • Graphene oxide drug carrier as well as preparation method and application thereof
  • Graphene oxide drug carrier as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] This embodiment provides a graphene oxide drug carrier GO-PEG-OSA, the preparation method of which comprises the following steps:

[0051] (1) Preparation of graphene oxide dispersion

[0052] Weigh a certain amount of micron-sized graphene oxide, disperse it into distilled water to prepare a suspension with a mass concentration of 10mg / ml, and then use an ultrasonic disperser to ultrasonicate intermittently at 4°C for 24h with an ultrasonic power of 600w; then centrifuge at 5000rpm for 10min, The supernatant was collected and then freeze-dried to obtain nano-graphene oxide with a particle size of about 100 nm.

[0053] Take a certain amount of nanometer graphene oxide, disperse with distilled water, be mixed with the nanometer graphene oxide dispersion liquid that mass concentration is 5mg / ml. Add the sodium hydroxide solution of 1mol / L then, adjust the pH of dispersion to 10, magnetically stir 1 hour at room temperature, centrifuge at 6000rpm then, discard supernatan...

Embodiment 2

[0067] The present embodiment provides a kind of nano medicine, and its preparation method is as follows:

[0068] Get a certain amount of GO-PEG-OSA of embodiment 1 and be dispersed in distilled water, be mixed with the GO-PEG-OSA dispersion liquid that concentration is 5mg / L. Take 2ml of GO-PEG-OSA dispersion, add 200μL of PTX solution, stir the mixture at 4°C for 12h, then centrifuge at 10000rpm for 20min. Subsequently, the supernatant was collected and the content of free PTX was measured by high performance liquid chromatography (HPLC). The precipitate was dispersed again with distilled water and freeze-dried to obtain the PTX-loaded nanomedicine PTX@GO-PEG-OSA.

[0069] As a comparison, when GO-PEG-OSA is replaced with GO-PEG prepared in step (2) of Example 1 during the preparation of nanomedicine, another nanomedicine PTX@GO-PEG can be obtained.

[0070] performance testing

[0071] (1) Sustained release effect

[0072] According to different release environments, t...

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Abstract

The invention provides a graphene oxide drug carrier as well as a preparation method and application thereof. The preparation method of the graphene oxide drug carrier comprises the following steps: (1) adding polyethylene glycol diamine into a dispersion liquid of graphene oxide, and carrying out amide condensation reaction under the action of an activating agent to obtain GO-PEG; (2) dissolvingGO-PEG in a solvent to prepare a GO-PEG dispersion liquid, adding oxidized sodium alginate, and carrying out Schiff base reaction under the action of an activating agent; and after the reaction is finished, purifying and drying to obtain the graphene oxide drug carrier GO-PEG-OSA; dispersing GO-PEG-OSA in a solvent to prepare a GO-PEG-OSA dispersion liquid, adding the drug solution, stirring for 10-20 h, and centrifuging to obtain a nano drug. The drug carrier and the nano drug are sensitive to pH and photo-thermal, have pH / thermal dual intelligent response release characteristics, have excellent biocompatibility, stability and water solubility, and can efficiently and quickly carry the drug into a focus.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, in particular to a graphene oxide drug carrier and its preparation method and application. Background technique [0002] Traditional treatment options for malignant tumors mainly include surgical resection, chemotherapy, and radiation therapy. Most patients are locally advanced when diagnosed, and chemotherapy is one of the inevitable main methods. In addition to the side effects caused by chemotherapy on the normal tissues of patients, tumor drug resistance is a major clinical problem that cannot be ignored. Once a patient develops chemoresistance, the only option is to replace the chemotherapeutic drug or increase the dose, which will undoubtedly increase the side effects of the patient, reduce the compliance of treatment, and the curative effect is still uncertain. In order to overcome the drug resistance of tumors, improve the therapeutic effect of tumors, and prolong the survi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K47/18A61K47/04A61K41/00A61K31/337A61P35/00C08G83/00C08G81/00
CPCA61K31/337A61K41/0052A61K41/0057A61K47/02A61K47/18A61K47/36A61P35/00C08G81/00C08G83/001A61K2300/00
Inventor 冯晓黎郭伟洪陈志安陈祈月张雅晴
Owner 南方医科大学口腔医院
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