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Anti-protein adsorption self-cleaning block copolymer, preparation method and application thereof

A block copolymer, anti-protein adsorption technology, applied in the preparation methods of peptides, chemical instruments and methods, production of bulk chemicals, etc., can solve the problems of protein adsorption and cleaning difficulties of coating materials, etc. Solve the effect of coating cleaning, simple and fast preparation process

Active Publication Date: 2022-07-05
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a block copolymer with dual effects of anti-protein adsorption and self-cleaning, which can be used as a coating material for capillary electrophoresis, and solve the problem of difficult protein adsorption and cleaning of existing capillary wall coating materials

Method used

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  • Anti-protein adsorption self-cleaning block copolymer, preparation method and application thereof
  • Anti-protein adsorption self-cleaning block copolymer, preparation method and application thereof
  • Anti-protein adsorption self-cleaning block copolymer, preparation method and application thereof

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preparation example Construction

[0044] The present invention also provides a method for preparing an anti-protein adsorption self-cleaning block copolymer, comprising the following steps:

[0045] S1. Under the protection of nitrogen atmosphere, add 2-bromoisobutyryl bromide BIBB, triethylamine ETA and lutidine DMAP into an appropriate amount of organic solvent dichloromethane DCM, stir and dissolve and place in an ice-water bath to obtain Mixed solution B; mixed polyethylene glycol monomethyl ether PEO and DCM in a mass ratio of 1:(3~5) to obtain mixed solution C, then added mixed solution C dropwise to mixed solution B, and reacted at room temperature for 10~ 20h, extract three times with NaCl solution, dry the organic phase over anhydrous magnesium sulfate, filter, and rotate to remove the solvent, the product is settled in glacial ether, filtered and dried in vacuum to obtain a light yellow macroinitiator PEO-Br; among which PEO is preferred for PEO 2000 ;

[0046] S2. Add the initiators PEO-Br, NIPAAm...

Embodiment 1

[0060] The preparation method of the anti-protein adsorption self-cleaning block copolymer provided in this embodiment comprises the following steps:

[0061] S1. Add 1.22g DMAP, 1.24ml BIBB, 1.4ml TEA and 15ml DCM to a 150ml three-necked flask, stir and dissolve in an ice-water bath, under nitrogen protection, add 10g PEO dropwise with a constant pressure dropping funnel 2000 50 ml of DCM solution was slowly stirred at room temperature for 12 h. After the reaction was completed, it was extracted three times with NaCl solution, the organic phase was dried over anhydrous magnesium sulfate, filtered, and the solvent was removed by rotary evaporation. The product was precipitated in glacial ether, filtered and dried in vacuum for 12 hours to obtain a light yellow ATRP macroinitiator PEO 2000 -Br.

[0062] S2, the initiator prepared in step S1 0.5g PEO 2000 -Br, 2.2632g NIPAAm, 0.043g PMDETA were added to a 150ml three-necked flask, and 10ml of dimethylformamide DMF and H were a...

Embodiment 2

[0066] The preparation method of the anti-protein adsorption self-cleaning block copolymer provided in this embodiment comprises the following steps:

[0067] S1. Add 1.22g DMAP, 1.24ml BIBB, 1.4ml TEA and 15ml DCM into a 150ml three-necked flask, stir and dissolve in an ice-water bath, under nitrogen protection, add 25g PEO dropwise with a constant pressure dropping funnel 5000 50 ml of DCM solution was slowly stirred at room temperature for 12 h. After the reaction was completed, it was extracted three times with NaCl solution, the organic phase was dried over anhydrous magnesium sulfate, filtered, and the solvent was removed by rotary evaporation. The product was precipitated in glacial ether, filtered and dried in vacuum for 12 hours to obtain a light yellow ATRP macroinitiator PEO 5000 -Br.

[0068] S2, the initiator prepared in step S1 1.25g PEO 5000 -Br, 2.663g NIPAAm, 0.043g PMDETA were added to a 150ml three-necked flask, and 10ml of dimethylformamide DMF and H were...

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Abstract

The invention discloses an anti-protein adsorption self-cleaning block copolymer, a preparation method and an application thereof. The block copolymer is composed of polyethylene glycol monomethyl ether PEO, N-isopropylacrylamide NIPAAm, methacrylic acid 3-propyl sulfonate SPMAP three hydrophilic materials are used as raw materials, which are prepared by atom transfer radical polymerization method ATRP on a macromolecular initiator. Each block in the triblock copolymer PEO‑PNIPAAM‑PSPMAP has good hydrophilicity, which guarantees the anti-protein adsorption performance, and the temperature-sensitive block part provides the basis for the self-cleaning performance. The copolymer and the coupling agent are cross-linked in the aqueous phase by means of layer-by-layer self-assembly to form a double-effect coating capillary with anti-protein adsorption and self-cleaning, which has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of antifouling coatings, in particular to an anti-protein adsorption self-cleaning block copolymer, a preparation method and an application thereof, which can be used as a capillary coating for electrophoretic separation and analysis of proteins, so as to realize the protection of the coating. Self-cleaning. Background technique [0002] Proteins are biologically active macromolecules composed of amino acids. In the biological environment, non-selective adsorption of proteins on the surface of materials will cause non-directional adsorption, which is the main cause of surface contamination of materials. Materials in biomedical applications should try to avoid causing immune responses in the body, so they have higher requirements. However, the problem of protein adsorption in the field of chemical analysis also affects the sensitivity and analytical efficiency of device detection. [0003] Capillary electro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F293/00C08F220/54C08F220/38C09D153/00C09D5/16C07K1/26
CPCC08F293/005C08F220/54C08F220/382C09D153/00C09D5/1662C07K1/26C08F2438/01Y02P20/54
Inventor 于冰丛海林王芳申有青
Owner QINGDAO UNIV
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