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Application of traditional Chinese medicine composition in preparation of medicine for preventing and/or treating myocardial ischemia reperfusion injury

A technology for reperfusion injury and myocardial ischemia, applied in the direction of drug combination, cardiovascular system diseases, plant/algae/fungus/moss components, etc., can solve limited problems and achieve good biocompatibility

Inactive Publication Date: 2020-08-11
GUIYANG XINTIAN PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in the prior art, strategies for preventing or treating myocardial ischemia-reperfusion injury are still very limited. Therefore, it is very meaningful to develop a new strategy for preventing or treating myocardial ischemia-reperfusion injury

Method used

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  • Application of traditional Chinese medicine composition in preparation of medicine for preventing and/or treating myocardial ischemia reperfusion injury
  • Application of traditional Chinese medicine composition in preparation of medicine for preventing and/or treating myocardial ischemia reperfusion injury
  • Application of traditional Chinese medicine composition in preparation of medicine for preventing and/or treating myocardial ischemia reperfusion injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Experimental grouping and establishment of MIRI rat model:

[0076] (1) Experimental grouping

[0077] After 1 week of adaptive feeding, 96 rats were randomly divided into 8 groups, 12 in each group, respectively OVXsham group (ovariectomy sham operation group), OVXsham+I / R group (ovariectomy sham operation + ischemia-reperfusion group) ), OVX group (ovariectomy group), OVX+I / R group (ovariectomy+ischemia-reperfusion group), OVX+I / R+NS group (normal saline group 0.5mL / 100g / d), OVX +I / R+KTL group (low dose treatment group 0.4g / Kg / d), OVX+I / R+KTM group (medium dose treatment group 0.8g / Kg / d), OVX+I / R+KTH group ( High dose treatment group 1.6g / Kg / d).

[0078] The normal saline group refers to the use of 0.5mL of normal saline per 100g rats every day; the low dose treatment group refers to the use of 0.4g medicine per Kg rats every day; 0.8g of drug was administered; the high-dose treatment group refers to the administration of 1.6g of drug per Kg rat per day.

[0079] ...

Embodiment 2

[0086] Heart TTC staining experiment, the specific method is as follows:

[0087]TTC staining principle: TTC is a fat-soluble light-sensitive complex. Its main component exists in the respiratory chain and is the proton acceptor of the pyridine-nucleoside structural enzyme system. In mammals, the dehydrogenase of normal tissues can be combined with The complex is reversed and appears red. If the tissue ischemia, the dehydrogenase activity in it will not change, and it will appear white to the naked eye. The heart specimens collected in each group in Example 1 were put into a -20°C refrigerator for quick freezing for about 20 minutes, which was convenient for slicing. Holding an ultra-thin blade, slice from the apex of the heart, the cutting plane is perpendicular to the long axis of the heart, and end at the bottom of the heart. The thickness of the thin slice of heart tissue is about 2mm. The tissue was placed in 2% TTC staining solution, wrapped in tinfoil and protected fro...

Embodiment 3

[0094] Heart HE staining experiment, the specific method is as follows:

[0095] The heart tissues collected from each group in Example 1 were fixed in 4% paraformaldehyde solution for 24 hours, and then dehydrated overnight to prepare paraffin-embedded specimens, which were sectioned for use. Put the slices into the dewaxing solution for dewaxing, a total of 3 times, 5min each time. The slices were hydrated, passing through anhydrous ethanol, 95% ethanol and 75% ethanol solutions in sequence according to the concentration gradient, each time for 2 minutes, and rinsed with distilled water after the end. Stain with hematoxylin for 5 minutes, rinse with tap water for 3 times, each time for 1 minute, and observe the situation of nuclear blue staining under the microscope. Alcohol differentiation with 1% dilute hydrochloric acid for 5s, rinsed with tap water for 1min. 1% dilute ammonia for 2 seconds, rinse with tap water for 1 minute. Stain with eosin for 1 min, rinse with tap ...

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Abstract

The invention relates to application of a traditional Chinese medicine composition in preparation of a medicine for preventing and / or treating myocardial ischemia reperfusion injury. The traditional Chinese medicine composition is prepared from radix rehmanniae praeparata, rhizoma coptidis, radix paeoniae alba, radix scutellariae, colla corii asini and poria. The traditional Chinese medicine composition can obviously control the weight gain of a rat after ovariectomy and can obviously reduce the infarction area of the rat under myocardial ischemia-reperfusion injury after ovariectomy; the levels of serum LDH and serum CK after myocardial ischemia reperfusion are remarkably reduced, and myocardial damage is reduced; and according to the application, the expression of GRP78 protein, CHOP protein, Active-caspase12 protein and the Active-caspase3 protein after the myocardial ischemia reperfusion can be significantly inhibited and reduced, so as to inhibit the apoptosis of the myocardial cells after the myocardial ischemia reperfusion.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, relates to a new application of a traditional Chinese medicine composition, in particular to the application of a traditional Chinese medicine composition in the preparation of medicines for preventing and / or treating myocardial ischemia-reperfusion injury, and especially provides a traditional Chinese medicine composition Application in preparation of medicine for preventing and / or treating myocardial ischemia-reperfusion injury after ovariectomy. Background technique [0002] The serious consequence of cardiovascular disease is the occurrence of ischemic heart disease. With the extensive development of coronary artery intervention and thrombolytic therapy, myocardial ischemia-reperfusion injury has become the obstacle that hinders ischemic myocardium from obtaining the best curative effect from reperfusion therapy. The main problem is to prevent and alleviate myocardial isc...

Claims

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Application Information

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IPC IPC(8): A61K36/804A61P9/10A61K35/36
CPCA61K36/804A61K36/718A61K36/71A61K36/65A61K36/539A61K35/36A61K36/076A61P9/10A61K2236/331A61K2236/39A61K2236/51
Inventor 张杰金春慧
Owner GUIYANG XINTIAN PHARMA CO LTD
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