Reversible syringomyelia animal model, and construction method and application thereof
A syringomyelia and animal model technology, applied in veterinary surgery, veterinary instruments, medical science, etc., can solve the problem of inability to dynamically observe the proliferation, differentiation and migration characteristics of endogenous neural stem cells in the spinal cord, sudden changes in the microenvironment, neurological function, etc. Unable to restore and other issues
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[0031] Embodiment 1: rat model
[0032] Fifty female 8-week-old Sprague-Dawley rats from the animal room of Xuanwu Hospital, Capital Medical University, China were used as subjects for the study, and the rats weighed from 230 grams to 280 grams. The experimental group consisted of 40 rats who received epidural compression surgery. In the control group, 10 rats underwent sham operation. General anesthesia with enflurane, nitrous oxide and oxygen mixed anesthesia. Anesthesia induction was performed in anesthesia room with a dose of 2% enflurane, 70% nitrous oxide and 30% oxygen. Anesthesia was maintained using a rat breathing mask during the operation.
[0033] Rats were placed in a prone position, and their limbs were fixed on a constant temperature operating bed at 36°C. After the back hair was shaved, 1% povidone iodine solution was used for skin disinfection. With T13 as the center, make a longitudinal skin incision along the middle of the back with a length of about 3 cm....
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