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Ginseng glycoprotein and its preparation method and application

A technology of glycoprotein and ginseng, applied in the field of medicine, can solve the problems of development and utilization limitations, and achieve the effect of improving spermatogenesis and sperm motility.

Active Publication Date: 2022-02-08
CHANGCHUN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, the development and utilization of ginseng polysaccharides in the fields of medicine and health food have been limited due to the fact that the biological activity of ginseng polysaccharides is limited to improving immunity

Method used

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  • Ginseng glycoprotein and its preparation method and application
  • Ginseng glycoprotein and its preparation method and application
  • Ginseng glycoprotein and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 test sample

[0040] 1.1 Preparation of ginseng glycoprotein

[0041] (1) Take 5kg of ginseng, pulverize it into pieces, add 8 times the amount of 80% ethanol, heat it to 70-80°C for reflux extraction, filter, then use 8 times the amount of 80% ethanol for reflux extraction, filter, and combine twice Filtrate, recovered ethanol to obtain 10L of concentrated solution, the concentrated solution was separated by macroporous resin D101, eluted with pure water, the eluate was collected, concentrated, and 10L of alcohol-extracted ginseng glycoprotein concentrated solution was obtained;

[0042] (2) Decoct the ginseng herb residues after two times of alcohol reflux in step (1) with pure water twice, add 10 times the amount of water of ginseng herb residues for the first time, decoct for 2 hours, and filter; Decoct 10 times the amount of ginseng dregs in water for 2 hours, filter, combine the two decoctions, and concentrate to 10L to obtain a wa...

Embodiment 2

[0049] The physicochemical property analysis of embodiment 2 test samples

[0050] 2.1 Determination of physical and chemical properties of ginseng glycoprotein

[0051] The neutral sugar content of ginseng glycoprotein was determined by the phenol-sulfuric acid method with glucose as the standard; the acidic sugar content of ginseng glycoprotein was determined by the m-hydroxybiphenyl method with glucuronic acid as the standard; As a standard, determine the protein content in ginseng glycoprotein. The results are shown in Table 1.

[0052] 2.2 Physical and chemical properties of ginseng water extract and total ginseng saponins

[0053] (1) Determination of total sugar content in ginseng water extraction

[0054] Preparation of reference substance solution: Accurately weigh 10 mg of glucose reference substance dried at 105°C to constant weight, put it in a 100mL measuring bottle, add water to dissolve and dilute to the mark, shake well to obtain (100μg of glucose per 1mL). ...

Embodiment 3

[0065] The mensuration of embodiment 3 ginseng glycoprotein molecular weight

[0066] The HPLC method was used to make a standard curve with L-tryptophan, carbonic anhydrase, protease inhibitor, vitamin B12, and cytochrome C, and the molecular weight distribution was calculated with GPC software. The chromatographic conditions are as follows: chromatographic column: Sepax SRT SEC-100HQ gel chromatography column (8mm×300mm); mobile phase: phosphate buffer solution with pH 7.0; flow rate: 0.5mL / min; column temperature: 35°C; : UV 280nm; injection volume: 10 μL. The result is as figure 1 As shown, the weight average molecular weight of ginseng glycoprotein is between 9000-15400Da.

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Abstract

The invention provides ginseng glycoprotein and its preparation method and application. The weight average molecular weight of the ginseng glycoprotein is 9000-15400Da, the protein part is composed of 17 specific amino acids, and the sugar part is composed of 4 kinds of monosaccharides. The invention proves that the ginseng glycoprotein has the effect of treating oligoasthenospermia through pharmacodynamic experiments. In the invention, FITC is used to fluorescently label ginseng glycoprotein, and the tissue distribution and targeting efficiency of the drug are observed by mouse whole-body imaging technology. The experimental results show that ginseng glycoprotein has a tendency to target the testis tissue. The preparation process of the ginseng glycoprotein provided by the invention is simple, the relative yield is relatively high, the manufacturing cost is reduced, and the invention has good research and application value.

Description

technical field [0001] The invention relates to the field of medicine, in particular to ginseng glycoprotein and its preparation method and application. Background technique [0002] With the increasingly serious global environmental pollution and the influence of life pressure and other factors, oligospermia and asthenospermia have become a common disease of male fertility. In recent years, research on the treatment of oligospermia and asthenospermia has progressed rapidly. Modern medicine mainly starts with assistive technology for the treatment of this disease, but its popularization is limited due to its high technical requirements, high price and low success rate. And traditional Chinese medicine shows unique advantage in treating the disease of this aspect. TCM dialectics emphasizes that the pathogenesis of oligospermia is caused by deficiency of kidney yang and deficiency of kidney qi. Clinical treatment is divided into 5 disease types: (1) Kidney Qi Deficiency Synd...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C07K1/36C07K1/34C07K1/16C07K1/14A61K38/16A61P15/00A61K47/42
CPCC07K14/415A61K38/168A61P15/00A61K47/42
Inventor 罗浩铭陈英红韦康朱迪夫房晓雪单梦瑶田建明姜瑞芝
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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