Breeding method of new ganoderma lucidum variety with high triterpene content

Abstract

The invention provides a breeding method of a new ganoderma lucidum variety with high triterpene content. The breeding method comprises the following steps of S1, selecting wild ganoderma lucidum as aparent, carrying out isolated culture by a ganoderma lucidum tissue isolation method to obtain pure mycelia, and carrying out cultivation tests and screening to obtain a ganoderma lucidum variety with relatively high sporulation quantity; S2, preparing the obtained ganoderma spores into a spore suspension liquid, and equally dividing the spore suspension liquid into two parts; and S3, mutagenizing one part of the spore suspension liquid through an ultraviolet lamp. The breeding method of the new ganoderma lucidum variety with high triterpene content provided by the invention comprises the following steps of selecting wild ganoderma lucidum as the parent, respectively carrying out ultraviolet mutagenesis and low-energy nitrogen ion mutagenesis on the spore suspension liquid, respectively culturing, and hybridizing the two stable mutagenic ganoderma lucidum to generate the new ganoderma lucidum variety; continuous culture of at least 20 generations is carried out, and the stable ganoderma lucidum sub body is generated to be used as the parent; and the method has the advantages of the two mutagenic ganoderma lucidum are included, and the content of beneficial substances such as ganoderma lucidum triterpenoids and the like is increased in the ganoderma lucidum.

Description

technical field [0001] The invention relates to the field of ganoderma breeding, in particular to a method for breeding new ganoderma varieties with high triterpene content. Background technique [0002] Ganoderma lucidum is also known as Ruicao, Shenzhi, Xiancao, Yaocao, Huanyangcao, Linzhongling, Ganoderma lucidum, Wannian mushroom, Lingcao, Chizhi, Danzhi, Qiongzhen, etc. It is a polyporaceae fungus The fruiting body of Ganoderma lucidum. Its shape is umbrella-shaped, and the cap is kidney-shaped, semicircular or nearly circular. Ganoderma lucidum, the name of Chinese herbal medicine. This product is the fruiting body of Ganoderma lucidum. Harvested throughout the year, dried in the shade or sun. Functions and indications are: nourishing and strong. It is used to strengthen the brain, reduce inflammation, diuresis, and benefit the kidney. Ganoderma lucidum is recorded in "Compendium of Materia Medica" compiled by Li Shizhen, a great medical scientist in the Ming Dyna...

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a method for breeding new ganoderma varieties with high triterpene content, which solves the problem of ganoderma lucidum mutagenesis by ultraviolet rays or nitrogen ions, but the ganoderma varieties produced by these two mutagenesis methods only have their own advantages and cannot Further improve the content of beneficial substances such as triterpenoids in Ganoderma lucidum

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