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Application of bacitracin A in preparation of medicine for preventing and treating corona virus

A coronavirus and bacillus technology, applied in antiviral agents, drug combinations, antipyretics, etc., can solve the problems of no antiviral drugs and vaccines, and the in vivo efficacy is still under observation.

Active Publication Date: 2020-07-17
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there is no definite and effective antiviral drug and vaccine
From the analysis of the in vitro antiviral activity data of these drugs, the antiviral IC of most drugs 50 It is between low micromolar and medium micromolar, and the efficacy in vivo is still under observation

Method used

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  • Application of bacitracin A in preparation of medicine for preventing and treating corona virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Virus Amplification

[0030] African green monkey kidney cells (VeroE6) were divided into 3×10 5Each well was inoculated into a 96-well plate in Eagle’s minimum basal medium (minimum Eagle’s medium, MEM; GibcoInvitrogen) containing 10% fetal bovine serum (FBS; GibcoInvitrogen) at 37°C, 5% CO 2 Cultivate until the monolayer grows. Inoculate a 100-fold dilution of the clinical isolate of the novel coronavirus pneumonia into a 96-well plate full of monolayer cells, and place at 37°C, 5% CO 2 Culture for two days (including the normal control group).

[0031] Two days later, the lesion degree reached more than 75%. Put it in a -80°C ultra-low temperature refrigerator, freeze and thaw once repeatedly, collect the virus liquid amplified by the cells, centrifuge at 3000r / min for 30 minutes, remove the sediment, put it in a small tube and put it in a -80°C ultra-low temperature freezer Long-term preservation.

Embodiment 2

[0032] Example 2 Bacillus Phthalin A Drug Toxicity Evaluation

[0033] After dissolving the Bacillus Phthalin A powder in DMSO, add culture medium to dilute to 20 mg / mL, the final concentration of DMSO is 1%, filter through a 0.22 μm filter membrane and store at 4°C; after filtering, store at 4°C. Press about 2.5×10 per hole 4 Cells were seeded into a 96-well plate, and after 24-48 hours, when the cells had grown into a single layer, the culture medium was discarded, and 100 μL / well of drugs of different dilutions were added, and 100 μL / well of MEM was added to the control wells of normal cells, at 37°C in 5% CO 2 Continue culturing for 2 to 5 days, add 20 μL of CCK8 solution (5 mg / mL) to each well, and place at 37°C in 5% CO 2 Continue to incubate for 4 hours in the incubator. Discard the culture supernatant, add 100 μL dimethyl sulfoxide (DMSO) to each well, and shake at low speed for 10 minutes to fully dissolve the crystals. Select a wavelength of 490nm, and measure the...

Embodiment 3

[0034] Example 3 Efficacy Evaluation of Bacillus Phthalin A Against New Coronary Pneumonia Novel Coronavirus

[0035] To evaluate the antiviral efficacy of the drug, VeroE6 cells were grown at a density of 5×10 4 Cells / well were cultured overnight in a 48-well cell culture dish. Add virus (MOI 0.05) to infect for 2 hours. Then add 2-fold gradient dilution of the drug, each concentration set 4 duplicate wells, with 200 μM as the initial concentration of the drug, at 34 ° C, 5% CO 2 Incubate for 2 days in the incubator. Record the cytopathic effect (Cytopathogenic Effect, CPE. The occurrence of CPE in the cells is recorded according to the 6th grade standard. After recording the CPE, stain with the CCK8 method to determine the OD value. Use the Reed-Muench method to calculate the half effective concentration of the drug (EC 50 ), the half effective concentration (EC 50 ) is 2.38 μM, the half effective concentration is low, indicating that it has a high antiviral effect.

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Abstract

The present invention relates to the application of bacitracin A in preparation of a medicine for preventing and treating corona virus, and especially provides application of the bacitracin A or pharmaceutically acceptable salt thereof, isotope, stereoisomers, stereoisomer mixture, tautomer, ester, amide or prodrug in preparation of a medicine for preventing and / or treating diseases caused by thecorona virus. The corona virus is novel corona virus SARS-Cov-2, SARS-CoV, HCoV 229E, NL63, OC43, HKU1 and MERS-CoV. The median effective concentration (EC50) of the bacitracin A to the new corona virus (SARS-Cov-2) is 2.38 [mu]M, and the toxicity is low, which indicates that the bacitracin A has a good therapeutic window.

Description

technical field [0001] The invention belongs to the field of antiviral drugs, in particular to the application of bacillin A in the preparation of drugs for preventing and treating anti-coronavirus. Background technique [0002] New Coronary Pneumonia (COVID-19) is an infectious disease caused by a novel coronavirus (SARS-Cov-2) infecting the human body. Its symptoms mainly include fever, fatigue, dry cough, dyspnea, and renal failure [The Lancet, 2020, 395(10223): 507-513; The Lancet, 2020, 395(10223): 497-506]. Coronavirus belongs to Coronaviridae (Coronavirus) in systematic classification. Viruses of the genus Coronavirus are positive-strand single-stranded RNA viruses with an envelope (envelope), with a diameter of about 80-120nm. Its genetic material is the largest among all RNA viruses, and generally only infects humans, mice, pigs, cats, and dogs. , Avian vertebrates. Coronaviruses were first isolated from chickens in 1937. Coronavirus particles are irregular in s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/12A61P31/14A61P11/00A61P29/00A61P11/14A61P13/12
CPCA61K38/12A61P31/14A61P11/00A61P29/00A61P11/14A61P13/12
Inventor 袁曙光陈显翀罗木鹏崔文强邹荣峰赵金存孙静
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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