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A kind of weed biocontrol bacteria and its application

A technology for weeds and fungi, applied in applications, biocides, microorganisms, etc., can solve the problems of small amount of bacteria and general control effect, and achieve the effect of broad herbicidal spectrum and environmental safety.

Active Publication Date: 2022-03-08
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the current problem that the number of biocontrol bacteria for weed control is small and the control effect is general, the present invention provides a fungus of the genus Pyrenopsis, which can efficiently control grass weeds

Method used

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  • A kind of weed biocontrol bacteria and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Isolation and Identification of Bacterial Strains

[0022] 1. Isolation and Screening of Strains

[0023] The leaves of naturally susceptible weeds were collected in the field, and the diseased and healthy joint parts of the diseased leaves were cut off, washed 3 times with sterile water, and sterilized with 5% (v / v) sodium hypochlorite solution for 2 minutes, and then washed with 70% ( v / v) Alcohol surface disinfection for 1 minute, after rinsing with sterile water, transfer these tissues to an empty sterilized culture dish, dry them in an ultra-clean bench, and place them in a 25°C incubator for cultivation until the lesion grows out After the pathogenic bacteria, a small amount of mycelium at the edge was picked and transferred to a new petri dish for purification, and the obtained pure culture was inoculated with corresponding weeds for verification, and finally a total of 112 fungal strains were obtained.

[0024] The screening targets are crabgrass, bar...

Embodiment 2

[0033] Example 2 Determination of pathogenicity of bacterial strain MN115

[0034] Cultivate the MN115 strain on a PDA plate (Φ=9 cm), culture at 25°C for 7 days, wash the conidia with 6 mL of sterile water per plate; filter the conidia liquid with 3 layers of gauze, and configure 1×10 conidia according to the experimental requirements. 6 A / mL conidia liquid, used for the determination of control effect.

[0035] Plant crabgrass, barnyardgrass, goosegrass, wild oats, succulents, amaranthus and velvetleum and other weeds in the greenhouse, spray the weeds on the weeds according to the water consumption of 45L per mu, and after 10 days according to the following formula, Observe and count the infection rate of the strains, and count the control effects of each strain on different weeds after 21 days:

[0036] Infection rate (%) = number of infected plants treated / total number of treated plants × 100%.

[0037] Inhibition rate of fresh weight (%) = (average fresh weight of cont...

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Abstract

The invention provides a weed biocontrol bacterium and application thereof. Described biocontrol bacteria belong to the fungus of the genus Pyrenomyces ( Peyronellaea glomerata ), and its deposit number is CGMCC NO.17471. The above-mentioned fungi of the genus Pyrenium can be used to control grass weeds such as goosegrass, crabgrass and wild oats. The fungus of the genus Pyrenium of the present invention is isolated from the leaves of weeds in the field, and the strains are isolated from the natural environment. Therefore, releasing them into nature will not cause any ecological and environmental hidden dangers, and is a safe biological control agent. body. The Pyrenomyces fungus of the present invention can infect a variety of grass weeds, and its pathogenicity is relatively rapid. After 3 days of inoculation, it can form obvious leaf scabs, and with the expansion of scabs, it can achieve the goal of effective prevention and control The purpose of weeds. The fungus is environmentally safe, has a wide herbicidal spectrum, and has great potential for further development.

Description

technical field [0001] The invention belongs to the field of biological control of weeds, and in particular relates to a fungus of the genus Pyrenomyces capable of infecting and controlling various grass weeds. Background technique [0002] The types of weeds in farmland in my country are complex. There are more than 1400 species of weeds in my country that have been reported, and about 60 breeding weeds can invade farmland and have a serious impact on agricultural production in my country. Most weeds have well-developed roots, strong environmental adaptability, strong seed production ability, and rapid spread, all of which make the effective control of weeds more difficult. According to statistics, the food loss caused by weed damage in our country is about 200 million tons every year. This does not include vegetables, fruit trees and other farmland crops affected by weeds. It can be said that weed damage has already threatened my country's farmland output. One of the impor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P13/02C12R1/645
CPCC12N1/14C12R2001/645C12N1/145
Inventor 李健李美高兴祥房锋
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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