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Application of gastrodin to medical titanium metal usage in diabetes environment

A diabetes and gastrodin technology, applied in the fields of medical science, pharmaceutical formulations, prostheses, etc., can solve the problems of poor osseointegration of titanium implants, affecting the biological function of cells, oxidative stress damage, etc., and achieve the effect of improving osseointegration. , to ensure the supply of drugs, reduce the effect of loosening and instability

Active Publication Date: 2020-07-10
GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: the present invention provides an application of gastrodin in the use of titanium metal implants in traditional Chinese medicine in diabetic environments, and its purpose is to solve the oxidative stress damage caused by diabetic environments to osteoblasts at the "titanium-bone" interface in the prior art , affect the biological function of cells and aggravate the damage of apoptosis, and finally lead to the clinical problems of poor osseointegration and long-term loosening of titanium metal implants, so as to improve the stability of medical titanium metal implants

Method used

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  • Application of gastrodin to medical titanium metal usage in diabetes environment
  • Application of gastrodin to medical titanium metal usage in diabetes environment
  • Application of gastrodin to medical titanium metal usage in diabetes environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] This example shows the application of gastrodin in the preparation of a drug for improving the adhesion function of "titanium-bone" interface osteoblasts in a diabetic environment, that is, the application of gastrodin in improving the adhesion state of osteoblasts on the surface of titanium metal in a diabetic environment is given. effect.

[0042] Experimental method: The medical titanium metal was cut and processed into titanium sheets, the surface was polished and cleaned and disinfected for later use. Primary osteoblasts from SD rat neonatal rat calvaria were isolated and cultured by trypsin and passaged. 4 Cells / ml were seeded on the titanium surface with the same number of cells to establish an in vitro "titanium-bone" interface model.

[0043] Divided into 5 groups:

[0044] 1) Normal serum group (normal group);

[0045] 2) Diabetic serum group (diabetes group);

[0046] 3) Diabetes + gastrodin 1 μM;

[0047] 4) Diabetes + gastrodin 5μM;

[0048] 5) Diabet...

Embodiment 2

[0053] This example shows the application of gastrodin in the preparation of a drug for improving the proliferation and differentiation of osteoblasts at the interface of "titanium-bone" on the surface of materials in a diabetic environment, that is, the effect of gastrodin on the surface of osteoblasts in a diabetic environment on titanium metal is given. Improvement of proliferative capacity and osteogenic differentiation level.

[0054] Experimental method: Osteoblasts were seeded on the surface of the titanium sheet for culture, and the grouping was the same as that in Example 1. On the 4th and 7th day of culture, MTT detection was performed on the cells on the surface of the titanium sheet, and on the 7th day of culture, ALP detection was performed on the cells on the surface of the titanium sheet.

[0055] Result: as Figure 4 , Figure 5 As shown, compared with the normal control group, the proliferation and differentiation of osteocytes in the diabetic group were sig...

Embodiment 3

[0058] This example shows the application of gastrodin in the preparation of a drug for inhibiting the pathological apoptosis of osteoblasts at the "titanium-bone" interface in a diabetic environment, that is, gastrodine is shown to reduce the apoptotic damage of osteoblasts on the surface of titanium metal in a diabetic environment protective effect.

[0059] Experimental method: Osteoblasts were seeded on the surface of titanium metal for culture, and the grouping was the same as that in Example 1. On the 7th day of culture, double-labeled staining with TUNEL and DAPI was used, and confocal microscopy was used to observe; Caspase-3 kit was used to detect the activity of apoptosis pathway protein Caspase-3, and to evaluate the effect of gastrodin on the apoptosis of osteoblasts in diabetic environment. Impact.

[0060] result: Image 6 The staining results of apoptotic cells in each group are shown, and the green images are TUNEL-labeled apoptotic cell nuclei. Figure 7 Fo...

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Abstract

The invention relates to an application of gastrodin to medical titanium metal usage in diabetes environment. After a medical material is implanted into a human body, tissue repair of a 'material-bone' interface mainly relates to integration of new bone tissue and materials, wherein the osseointegration effect of the materials is determined by bioactivity and function of osteoblast responsible forosteogenic differentiation on the materials. According to the application disclosed by the invention, through osteoblast adhesive culture on the surface of medical titanium metal, an in vitro 'titanium-bone' interface model is constructed; and the gastrodin of therapeutic dose is used for performing addition culture, so that the improvement effect of the gastrodin on the biological functions of the osteoblast in the 'titanium-bone' interface is proved. The clinical problems that in the prior art, oxidative stress damage is caused to the osteoblast in the 'titanium-bone' interface by the diabetes environment, biological functions of cells are influenced, apoptosis damage is worsened, and bad osseointegration of a titanium metal implant and loosening of the titanium metal implant after a long term are finally caused, are solved, and the stability of the medical titanium metal implant is improved.

Description

technical field [0001] The invention belongs to the field of medical biological materials, and particularly relates to the application of gastrodin to improve the osseointegration effect and long-term stability of medical titanium metal in a diabetic environment. Background technique [0002] Titanium is by far the most widely used bone implant material. However, in some populations with systemic metabolic diseases such as diabetic patients, the long-term instability rate of titanium implants is significantly increased. Studies have shown that the rate of spinal screw loosening in diabetic patients is as high as 30%, which is 10 times that of the normal population. The long-term instability of titanium metal implants causes great family and social burdens to patients. With the increasing number of diabetic patients, the clinical demand of such patients with titanium implants for internal fixation is also increasing. Therefore, it is an urgent clinical problem to elucidate ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/06A61L27/28
CPCA61L27/54A61L27/06A61L27/28A61L2300/232A61L2400/06A61L2300/412A61L2300/40A61L2300/606A61L2300/602A61L2420/04A61L2420/02
Inventor 马翔宇刘兵项良碧周大鹏马一鸣
Owner GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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