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Bacillus licheniformis and application thereof

A technology of Bacillus licheniformis and strains, applied in the application, bacteria, microorganism and other directions, can solve the problems of affecting application, poor water solubility or stability, and low efficacy, achieve good economic and social benefits, and improve disease resistance. Ability, health-enhancing effect

Active Publication Date: 2020-07-03
LUZHOU ZHENGTAI BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex composition of traditional Chinese medicine, the content of active ingredients is low, the water solubility or stability of some active ingredients of traditional Chinese medicine is not good, and the toxicity and side effects are too strong, resulting in low drug efficacy, which seriously affects their application and cannot achieve better results. therapeutic or health effects

Method used

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  • Bacillus licheniformis and application thereof
  • Bacillus licheniformis and application thereof
  • Bacillus licheniformis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation and identification of Bacillus licheniformis LZZT-002

[0021] The soil samples from the roots of Astragalus were collected, and the microorganism strains were separated and purified by the dilution plate method. Use culture characteristics and morphological observations to pick out a number of microbial strains, and identify the strains by Gram staining and 16srRNA gene rapid amplification and sequencing methods. The 16s rRNA gene identification uses strain DNA as a template, and PCR amplifies its 16S rDNA sequence. The primers are (27f): 5'-AGAGTTTGATCCTGGCTCAG-3', SEQ ID NO. 1; and (1492r): 5'-GGTTACCTTGTTACGACTT-3', SEQ ID NO.2. The PCR reaction program is: pre-denaturation at 95°C for 6 minutes, denaturation at 95°C for 30 seconds, annealing at 53°C for 30 seconds, extension at 72°C for 70 seconds, and 30 cycles. The amplified product was sequenced after electrophoresis to detect its purity. The obtained 16S rRNA gene sequence was compared by NCB...

Embodiment 2

[0022] Example 2 Detection of Xylanase Activity of Strain LZZT-002

[0023] LZZT-002 strain was fermented and cultured in liquid medium (tryptone 5g, oat xylan 5g, glucose 5g, sodium chloride 5g, distilled water 1000ml, pH7.2) for two days, then centrifuged to collect the bacterial cells and placed in PBS buffer , Use an ultrasonic disruptor at a power of 600W to disrupt the cells, and then centrifuge to obtain the supernatant, that is, to obtain the crude enzyme solution.

[0024] The detection of xylanase activity refers to the national standard (GB / T 23874-2009).

[0025] Standard curve preparation: Draw 4ml of 0.1M acetic acid-sodium acetate buffer (pH5.5), add 5.0ml DNS reagent, heat in a boiling water bath for 5 minutes, cool to room temperature, add water to make the volume up to 25ml, and make a standard blank. Prepare xylan standard solutions of 0.1mg / ml, 0.2mg / ml, 0.3mg / ml, 0.4mg / ml, 0.5mg / ml, 0.6mg / ml and 0.7mg / ml. Take 2ml of each standard solution, add 2ml of 0.1M acet...

Embodiment 3

[0029] Example 3 Detection of cellulase activity of strain LZZT-002

[0030] Strain LZZT002 was spot-planted on sodium carboxymethylcellulose medium and cultured for 48 hours. Dye with 0.2% Congo red for 30 minutes, then first wash off the dye solution with distilled water, then soak in 1mol / L NaCl for 1 hour, and finally fix the color with 5% (w / v) acetic acid solution. The formation of a colorless transparent circle around the colony proves that the bacteria secrete cellulase.

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Abstract

The invention discloses bacillus licheniformis and application thereof, and belongs to the technical field of biological fermentation and biotransformation. The bacillus licheniformis LZZT-002 disclosed by the invention has xylanase activity and cellulase activity, and can improve the yield of astragalus polysaccharide, astragalus saponin and derivatives thereof when being used for fermentation and transformation of an astragalus extract. A fermented radix astragali feed additive prepared by fermenting radix astragali through the strain LZZT-002 is an efficient microbial agent integrating probiotics and effective components of traditional Chinese medicines, has the characteristics of environmental friendliness, excellent performance and the like, and has good economic and social benefits.

Description

Technical field [0001] The invention relates to the technical field of biological fermentation and biological transformation, and more specifically to a strain of Bacillus licheniformis and its application. Background technique [0002] Astragalus is a traditional Chinese medicine. The main medicinal components of Astragalus are astragalus polysaccharides and astragalus saponins, which can improve immune function, have anti-oxidation, anti-radiation and anti-cancer, protect cardiovascular and cerebrovascular, and improve memory. Biocatalysis and transformation technology is an emerging biotechnology that uses the catalytic performance of microbial enzymes to transform known chemical structures into derivatives with novel structures or higher activity. Traditional Chinese medicines have complex ingredients and low content of active ingredients. Some active ingredients of traditional Chinese medicines have poor water solubility or stability, and their toxic and side effects are too...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/10
CPCA23K10/18C12N1/20C12N1/205C12R2001/10
Inventor 张兴良
Owner LUZHOU ZHENGTAI BIOLOGICAL ENG CO LTD
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