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Method and system for extracting polyhydroxyalkanoate by combining ultrasound at high temperature and high pressure

A polyhydroxyalkanoate, ultrasonic technology, applied in separation methods, chemical instruments and methods, chemical/physical/physical-chemical processes of applying energy, etc., can solve problems such as increased cost, deterioration, and decreased PHA polymerization degree.

Active Publication Date: 2020-06-30
JILIN COFCO BIOCHEM +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of the solvent used in the organic solvent extraction method is higher and causes more serious harm to human health and the environment; extracting PHA with acid-base or surfactants easily causes a certain degree of degradation of PHA, resulting in the polymerization of the obtained PHA decreased and worsened
And the purity and recovery rate of the PHA that adopts above-mentioned two kinds of direction extractions to obtain are lower
Moreover, the waste liquid produced by the above two methods needs additional sewage treatment process for treatment, which greatly increases the production cost.

Method used

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  • Method and system for extracting polyhydroxyalkanoate by combining ultrasound at high temperature and high pressure

Examples

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Embodiment approach

[0069] According to the present invention, the fermentation liquid of the polyhydroxyalkanoate can be the fermentation liquid of microorganisms that can be used to prepare polyhydroxyalkanoate conventionally in the art, preferably, the microorganism is a halophilic bacteria, for example, it can be a salt One of the genus Monas, according to a preferred embodiment of the present invention, the PHA fermentation strain is Halomonas sp.; more preferably, the PHA fermentation strain is Halomonas sp. Bacteria (Halomonas sp.) TD01, its preservation number is CGMCCNO.4353 (CN201010578858.8).

[0070] In the present invention, the fermentation medium used for the fermentation of the polyhydroxyalkanoate is not particularly limited, and may be a conventionally used fermentation medium in the art, and will not be repeated here.

[0071] In the present invention, the conditions for the fermentation of the polyhydroxyalkanoate are not particularly limited, and may be conventionally used fe...

preparation example 1

[0103] Halomonas (TD01, whose preservation number is CGMCC NO.4353 (CN201010578858.8)) was inoculated in the seed medium (yeast powder of 5g / L, peptone of 10g / L and sodium chloride of 60g / L) Under the conditions of 37°C and 200rpm, carry out primary activation culture, culture to OD 600 Reach about 4, obtain first-grade seed liquid;

[0104] Inoculate the primary seed liquid into the seed medium with an inoculation amount of 10% by volume, carry out secondary activation culture at 37°C and 200rpm, and cultivate to OD 600 Reach about 4, obtain secondary seed liquid, obtain fermented seed liquid.

[0105] Then the inoculum of 10 volume % is inserted into the initial fermentation medium (sodium chloride 50g / L, glucose 50g / L, corn steep liquor powder 15g / L, urea 2g / L, magnesium sulfate 0.2g / L) L, potassium dihydrogen phosphate 5g / L, trace element mother liquid I 10mL / L, trace element mother liquid II 3mL / L. The trace element mother liquid I and II refer to the cited patent CN201...

Embodiment 1

[0107] (1) The polyhydroxyalkanoate fermentation liquid prepared in Preparation Example 1 is centrifuged through a disc centrifuge, so that the fermentation liquid is divided into an underflow (water content 80%) rich in bacterial cells (the first bacterial cells) And the top flow of the fermentation raffinate (the first fermentation raffinate), the top flow of the fermentation raffinate enters the temporary storage tank of the fermentation raffinate.

[0108] (2) the underflow that is rich in the target thalline cell that step (1) obtains is washed 2 times with the water of equal volume, then pumps into the belt type vacuum filter and carries out filtration, the thalline after the filtration (water content 80%) ) (the second bacterium cell) enters the extraction tank, and the filtrate (the second fermentation raffinate) enters the fermentation raffinate temporary storage tank.

[0109] (3) Pump the mixed solution of the fermentation residue and the filtrate in the temporary s...

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Abstract

The invention relates to the technical field of PHA extraction and separation, and discloses a method and a system for extracting polyhydroxyalkanoate by combining ultrasound at high temperature and high pressure. The method includes the steps: (1) carrying out solid-liquid separation of fermentation broth of polyhydroxyalkanoate to obtain bacterial cells containing polyhydroxyalkanoate; (2) resuspending the bacterial cells to obtain a bacterial suspension, and then crushing the bacterial cells under ultrasonic, heating and pressurizing conditions to obtain slurry containing polyhydroxyalkanoate; and (3) carrying out second plate frame filtration on the slurry to obtain polyhydroxyalkanoate, wherein a polyhydroxyalkanoate layer is pre-coated on the surface of filter cloth of the second plate frame filter. By adopting the method disclosed by the invention, the yield and purity of PHA can be effectively improved, the polymerization degree of the obtained PHA is relatively high, and the production cost is reduced.

Description

technical field [0001] The invention relates to the field of extraction and separation of polyhydroxyalkanoate, in particular to a method and system for extracting polyhydroxyalkanoate under high temperature and pressure combined with ultrasound. Background technique [0002] Polyhydroxyalkanoate (PHA) is a general term for a class of polymer polyesters that are completely synthesized by microorganisms. PHA is biodegradable and biocompatible, so it is considered as an environmentally friendly material, which helps to solve the increasingly serious environmental pollution problem. [0003] Although the use of PHA can effectively avoid the environmental hazards caused by petrochemical plastics, at present, there are some problems in the extraction and separation of PHA. At present, the techniques for extracting and separating PHA mainly focus on two methods: organic solvent extraction and acid-base or surfactant treatment extraction. The cost of the solvent used in the organ...

Claims

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Application Information

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IPC IPC(8): B01J19/10B01D36/04C08G63/08C08G63/90
CPCB01D36/045B01J19/10C08G63/08C08G63/90
Inventor 佟毅李义郭元亨刘安妮李大勇刘海军彭超陈博陈国强
Owner JILIN COFCO BIOCHEM
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