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Cultivation method for novel variety by inducing mutation of tender stem tissues of epimedium koreanum

The technology of a Korean Epimedium and its cultivation method is applied in the field of cultivating new varieties that induce mutations in the tender stem tissue of Korean Epimedium. It can solve the problems of insufficient main components of icariin, rotten roots, pests and diseases, and endangered species. The effect of clear source of single plant, high mutation rate and high dominant mutation

Active Publication Date: 2020-06-23
TONGHUA NORMAL UNIV +1
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Problems solved by technology

[0005] In order to solve the above-mentioned problem that with the increasing demand of Epimedium Korean, the amount of collection and consumption has exceeded the regeneration capacity of natural resources, resulting in endangered species and the shortage of main components such as icariin in artificial cultivation of Epimedium Korean and serious degradation. Phenomena and problems such as root rot and serious pests and diseases in the cultivation process

Method used

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  • Cultivation method for novel variety by inducing mutation of tender stem tissues of epimedium koreanum
  • Cultivation method for novel variety by inducing mutation of tender stem tissues of epimedium koreanum
  • Cultivation method for novel variety by inducing mutation of tender stem tissues of epimedium koreanum

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[0028] The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0029] Such as figure 1 with 2 As shown, a new variety breeding method for inducing mutations of tender stem tissue of Epimedium koreanum includes the following steps:

[0030] 1Materials and methods

[0031] 1.1 Material selection and pretreatment

[0032] In early May, select the newly germinated tender stems of wild Epimedium koreanum, cut into 0.5cm stem segments, rinse with 75% ethanol (volume concentration) for 8 seconds, and then transfer to saturated sodium hypochlorite solution (mass concentration) to soak for 3 minutes, sterile Rinse 10 times with water, and absorb the surface moisture with sterile filter paper for later use.

[0033] 1.2 method

[0034] 1...

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Abstract

The invention belongs to the technical field of plant propagation, and specifically relates to a cultivation method for a novel variety by inducing mutation of tender stem tissues of epimedium koreanum. According to the cultivation method for the novel variety by inducing mutation of the tender stem tissues of the epimedium koreanum provided by the invention, with tender stems of the epimedium koreanum materials, by combination of ethyl methanesulfonate (EMS) with a plant tissue culture technology and adoption of methods of embryonic callus induction, redifferentiation, embryoid rejuvenation and the like, an artificially cultivated novel variety of the epimedium koreanum, which is strong in independence of each single plant, high in genetic information purity of each single plant and clearin single plant source, is obtained; and the cultivation method has important protection and ecological significance for amplification of an epimedium koreanum population; meanwhile, the cultivationmethod can be further applied to construction of an epimedium koreanum mutant library; and the novel variety cultivated by using the cultivation method provided by the invention can be directly used as a strain for cultivation and variety certification.

Description

Technical field [0001] The invention belongs to the technical field of plant propagation, and specifically relates to a new variety breeding method for inducing mutations of tender stem tissue of Epimedium koreanum. Background technique [0002] Epimedium koreanum (Epimedium koreanum Nakai) is a perennial herbaceous plant belonging to the genus Epimedium of the Berberiaceae family, which is concentrated in the Changbai Mountains of Northeast China. Under multigrowth forests or shrubs, 400-1500 meters above sea level, it likes to grow in places with loose soil, fertile soil and high humus content. Epimedium koreanum is a traditional Chinese medicinal material for tonic. It is included in the Pharmacopoeia of the People’s Republic of China. It is said to have the effects of warming the kidney and yang, strengthening the muscles and bones, and dispelling rheumatism. Atrophic spermatorrhea, premature ejaculation, urinary incontinence, cold joints, irregular menstruation and other sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/06A01H1/04
CPCA01H4/008A01H1/06A01H1/04
Inventor 顾地周冯姚朱俊义孙仁爽刘伟刘哲
Owner TONGHUA NORMAL UNIV
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