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Up-conversion nanoparticle and preparation method thereof, and application of up-conversion nanoparticle in non-contact temperature measurement

A non-contact temperature measurement and nanoparticle technology, applied in nanotechnology, nanooptics, nanotechnology, etc., can solve the problems of large particle size, low temperature measurement sensitivity, high toxicity, and achieve high sensitivity, good biocompatibility, Avoid direct contact effects

Active Publication Date: 2020-05-22
DEZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to low temperature measurement sensitivity, high toxicity, and large particle size, there are few reports on nanothermometers in vivo.

Method used

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  • Up-conversion nanoparticle and preparation method thereof, and application of up-conversion nanoparticle in non-contact temperature measurement
  • Up-conversion nanoparticle and preparation method thereof, and application of up-conversion nanoparticle in non-contact temperature measurement
  • Up-conversion nanoparticle and preparation method thereof, and application of up-conversion nanoparticle in non-contact temperature measurement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 CaF 2 :Yb 3+ / Er 3+ Preparation of nanoparticles

[0064] (1) Add 0.44396g Ca(NO 3 ) 2 4H 2 O (1.88mmol, 94%), the concentration of 0.05M Yb (NO 3 ) 3 2mL (0.1mmol, 5%), Er(NO 3 ) 3 0.4mL (0.02mmol, 1%), add 15mL of deionized water and stir to form a mixed solution.

[0065] (2) Weigh 0.3359 g (4 mmol, 200%) of NaF, add 15 mL of deionized water and stir to dissolve. The NaF solution was dropped into the above mixed solution under stirring.

[0066] (3) The obtained mixed solution was added into a polytetrafluoroethylene-lined autoclave, heated to 180° C. and kept for 12 hours, and then lowered to room temperature.

[0067] (4) The reacted solution was centrifuged (10000 r / min×10 min), washed once with deionized water and ethanol, and dried at 60° C. to obtain the final product.

Embodiment 2

[0068] Example 2 CaF 2 :Yb 3+ / Er 3+ Co-cultivation of nanoparticles with Hela cells

[0069] (1) Hela cells were seeded in a 96-well plate at a density of 5000 cells / well, and incubated in 5% CO 2 Incubate overnight at 37°C.

[0070] (2) Prepare different concentrations of CaF with the medium 2 :Yb 3+ / Er 3+ Nanoparticles (6.25, 12.5, 25, 50, 100μM) were mixed with Hela cells in 5% CO 2 Co-cultivate for 24 hours at 37°C.

[0071] (3) 10 μL of 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) was added to each well.

[0072] (4) After 4 hours, remove the solution in the wells, add 150 μL of dimethyl sulfoxide (DMSO) to each well, shake for 10 minutes, and detect the absorbance of the well plate at 490 nm with a microplate reader.

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Abstract

The invention provides an up-conversion nanoparticle and a preparation method thereof, and application of the up-conversion nanoparticle in non-contact temperature measurement. The molecular formula of the up-conversion nanoparticle is CaF<2>: Yb<3+> / Er<3+>. The preparation method comprises the following steps: mixing a Ca<2+> salt, a Yb<3+> salt, an Er<3+> salt and water, dropwise adding an aqueous solution in which NaF is dissolved, carrying out a hydrothermal synthesis reaction, and carrying out purifying to obtain the up-conversion nanoparticle. The up-conversion nanoparticle prepared by the invention has the characteristics of good biocompatibility, strong fluorescence emission and the like, can be excited by near-infrared light and realize non-contact fluorescence temperature measurement, and is particularly suitable for temperature measurement of living organisms, so the up-conversion nanoparticle has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation and non-contact temperature measurement, and in particular relates to an up-conversion nano particle, a preparation method thereof and an application in non-contact temperature measurement. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Rare earth luminescent materials can realize the conversion between different wavelengths, and have a wide range of applications in lighting, display, biology and other fields. According to the different lengths of excitation light and emission light, rare earth materials are divided into up-conversion materials and down-conversio...

Claims

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Application Information

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IPC IPC(8): C09K11/61B82Y20/00B82Y40/00G01K11/32
CPCC09K11/7773B82Y20/00B82Y40/00G01K11/32
Inventor 刘国锋许士才刘汉平刘健健李迎仙宋瑞洪田蒙
Owner DEZHOU UNIV
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