Prothrombin time detection test strip and preparation method thereof
A technology of prothrombin time and test strips, which is applied in the field of detection and can solve the problems of expensive instruments, complicated operations, and high costs
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[0043] A preparation method of a prothrombin time detection test strip, comprising the steps of:
[0044] Step 1, the preparation of reaction reagent;
[0045] Prepare materials according to the following formula: the formula includes: 4-6 parts of tissue factor, 0.4-0.5 parts of phosphatidylcholine, 0.1-5 parts of film-forming agent, 2-12 parts of stabilizer, 0.1-1 part of preservative , 0.5-1 parts of surfactant, 2-5 parts of coagulant;
[0046] Add tissue factor and phosphatidylcholine into the buffer and mix, then magnetically stir to disperse, then add film forming agent, stabilizer, preservative, surfactant, coagulant, use 100ml concentration of 0.1-1M, pH value is 6 -8 buffer is stirred, stirred for 2 hours, and stored in the refrigerator for use; as an example, the buffer includes: phosphate buffer, TRIS buffer, citrate buffer, MES buffer.
[0047] Step 2, soak the carrier film in the reaction reagent for 5 minutes; after drying, the reagent layer 400 is obtained, an...
Embodiment 1
[0051] Remove the erythrocyte separation layer 500
[0052] 1. Preparation of reagent layer 400 solution: Add 5g of rabbit brain powder and 0.5g of phosphatidylcholine into the buffer and mix, then magnetically stir to disperse, then add 2g of hydroxyethyl cellulose, 2g of bovine serum albumin, 1g Trehalose, 1g of polyethylene glycol 6000, and 0.1g of Proclin300, 0.5g of triton X-405, 2g of calcium chloride, 100ml concentration of 0.5M, pH value of 7.2 phosphate buffer after stirring for 2 hours , kept in the refrigerator for later use.
[0053] 2. Soak the glass fiber membrane in the above solution for 5 minutes, and dry it in a 45-degree oven for 30 minutes to form the reagent layer 400.
[0054] 3. Combine the prepared electrode 200, double-sided adhesive tape 300, reagent layer 400, diffusion membrane 600, and hydrophilic membrane 700 into a finished product 1.
Embodiment 2
[0056] Compared with embodiment 1, the erythrocyte separation layer 500 is increased
[0057] 1. Preparation of reagent layer 400 solution: Add 5g of rabbit brain powder and 0.5g of phosphatidylcholine into the buffer and mix, then magnetically stir to disperse, then add 2g of hydroxyethyl cellulose, 2g of bovine serum albumin, 1g Trehalose, 1g of polyethylene glycol 6000, and 0.1g of Proclin300, 0.5g of triton X-405, 2g of calcium chloride, 100ml concentration of 0.5M, pH value of 7.2 phosphate buffer after stirring for 2 hours , kept in the refrigerator for later use.
[0058] 2. Soak the glass fiber membrane in the above solution for 5 minutes, and dry it in a 45-degree oven for 30 minutes to form the reagent layer 400.
[0059] 3. Follow the method of 1 to assemble the prepared electrode 200, double-sided adhesive tape 300, reagent layer 400, erythrocyte separation layer 500, diffusion membrane 600, and hydrophilic membrane 700 to form the finished product 2 in Figure 2. ...
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