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Eupatorium catarium flower extract and application thereof

A technology of extracts and grass flowers, which is applied in the field of extraction and separation of active ingredients of natural plants, can solve the problems of no false stinky grass, no separation of chemical components of the effective parts of the false stinky grass flowers against Tenebrio molitor, etc., to achieve good insect resistance activity, The effect of low cost and simple process

Pending Publication Date: 2020-05-08
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there have been no patents related to the anti-insect application, and there is no report on the separation of the effective part of the anti-Tenbelia molitor and its further chemical components

Method used

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  • Eupatorium catarium flower extract and application thereof
  • Eupatorium catarium flower extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] The steps of the preparation method of the fenugreek flower extract are as follows:

[0025] (1) Add petroleum ether (60-90° C.) solvent to the dried and pulverized False stinky medicinal material (passed through a 20-80 mesh sieve), and extract by soaking at room temperature, heating or ultrasonic extraction, and the extraction time is 60 minutes to 10 minutes. 7 days, the ratio of solid to liquid is 1:1~1:10;

[0026] (2) Filtrating and concentrating under reduced pressure to obtain the petroleum ether-dissolved extract by filtering the medicinal material extract of Scutellaria fruticosa;

[0027] (3) The petroleum ether dissolved part is subjected to silica gel column chromatography, and pure petroleum ether is used as a solvent to elute and remove long-chain oil wax and other components in the extract, and then eluted with ethyl acetate to wash the column, and the effluent is concentrated in vacuum The obtained component is the part of petroleum ether determined to...

example 1

[0031] The preparation of the effective part of the example one scutellaria fragrans

[0032] After dried and pulverized in the shade, the fenugreek flower (15Kg) was soaked in petroleum ether (60-90°C) at room temperature for 3 times, 7 days each time. The extracts were combined and concentrated under reduced pressure to obtain the petroleum ether-dissolved part extract (97g).

[0033] After the petroleum ether dissolved part is concentrated, it is the effective part of the anti-insect part of the stinky flower. After repeated column chromatography on silica gel and Sephdex LH-20, 5 flavonoids and 2 sterols are obtained. , 1 benzoic acid compound, 1 sesquiterpenoid compound, 1 alkaloid compound, 1 pyrone compound and 3 chromogen compounds, respectively petroleum ether: ethyl acetate (15: 1) Petroleum ether: ethyl acetate (20:1) silica gel column chromatography to obtain 38 mg of compound 6,7,8,4′-tetramethoxyflavone (2), 2′-hydroxyl-4,4′, 5′,6′-tetramethoxychalcone (5) 53mg ...

Embodiment 2

[0036] Example 2 Study on Anti-Tenebrio Molitor Activity of Petroleum Ether Dissolved Part of Pseudomonas sinensis

[0037] 1. Experimental method:

[0038] (1) The contact killing effect of the petal ether extract of Pseudomonas molitor on Tenebrio molitor larvae

[0039] Using the method of soaking worms, pick 20 3rd instar larvae with basically the same size and put them into a self-made filter screen, soak in the treatment solution for 10 seconds, then take it out quickly, use filter paper to dry up the excess liquid, and place it with fresh cabbage or kale leaves. In the petri dish (diameter=9cm) that is padded with moisturizing filter paper, cover it with plastic wrap, and prick a few holes with a needle for ventilation, repeat each treatment 3 times, do the same treatment with the control solution, and observe the symptoms of the treated worms , then place the petri dish in an incubator with a temperature of 25°C and a humidity of 80%, investigate the death situation a...

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Abstract

The invention discloses a Eupatorium catarium flower extract and an application thereof. Dry powder of Eupatorium catarium flowers undergoes extraction by using a petroleum ether solvent; and the obtained extract undergoes filtration and reduced pressure concentration to obtain a petroleum ether dissolved part, and the obtained petroleum ether part is separated to obtain five flavonoid compounds,two sterol compounds, one benzoic acid compound, one sesquiterpenoid compound, one alkaloid compound, one pyrone compound and three chromene compounds. The prepared extract is high in yield, simple intechnological process, low in cost and suitable for large-scale preparation, has good insect-resistant activity and can be applied to auxiliary botanical insecticides.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of effective components of natural plants, and in particular relates to a preparation method of an extract of stinkweed flower and its anti-insect application. Background technique [0002] Praxelis clematidea (Griseb.) R.M.King&H.Rob., also known as chrysanthemum, is an annual herb of the family Compositae (Praxelis C), Eupatorium catarium Veldkamp (Eupatorium L) Is its synonym, native to South America. In China, false stinky grass was first discovered in Hong Kong in the 1980s, and is now widely distributed in roadsides, wasteland, farmland and grasslands in coastal areas such as Guangdong, Fujian, Hong Kong, Macao, Taiwan, and Hainan. It generally grows in hills and plains. False stinky grass has a strong ability to absorb soil nutrients, is easy to consume soil fertility, and seriously damages the soil's arability. It can also secrete a toxic and foul-smelling substance tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/40C07D311/30C07C49/84C07C45/78C07J9/00C07C65/21C07C47/46C07C235/08C07C231/24C07D309/36C07D311/04C07D311/70C07D311/58A01N43/16A01N35/04A01N45/00A01N37/40A01N37/20A01P7/04
CPCC07D311/40C07D311/30C07C49/84C07J9/00C07C65/21C07C47/46C07C235/08C07D309/36C07D311/04C07D311/70C07D311/58C07C45/78C07C51/42C07C231/24A01N43/16A01N35/04A01N45/00A01N37/40A01N37/20
Inventor 陈文豪杨健妮惠阳周秀悄黄丹
Owner HAINAN NORMAL UNIV
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