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A kind of cultivation method of wild ganoderma lucidum

A cultivation method and technique of Ganoderma lucidum are applied in the field of asexual reproduction of wild Ganoderma lucidum, which can solve the problems of the cultivation technique of Ganoderma lucidum cannot meet the needs of large-scale production, the quality of strains is poor, the survival rate of bacterial strains is not high, and the cost is low, easy to obtain, Guaranteed survival rate

Active Publication Date: 2021-12-17
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the domestication technology of wild ganoderma lucidum has the disadvantages of low breeding survival rate, poor quality of strains for production, and the inability of existing ganoderma cultivation technology to meet the needs of large-scale production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Acquisition of Ganoderma lucidum entities: In early August 2016, wild Ganoderma lucidum with good growth, no pests and diseases, and good color was collected as explants. Rinse 2-3 times, then wipe the Ganoderma lucidum with absorbent cotton dipped in a 1.5% volume concentration of detergent solution and set aside;

[0027] (2) Preparation of female species: with potato 100g, rice milk 10g, sugar 10g, agar 10g, water 50g as female seed culture medium, described rice milk is the product that rice and water beating obtain, and described rice and water weight ratio Put it into a test tube at a ratio of 1:2, put it into a test tube at a pressure of 103.4kPa and a temperature of 121.3°C, autoclave it for 15-20 minutes to prepare a potato agar test tube for use, and inoculate the collected wild Ganoderma lucidum fruiting bodies by tissue separation Put it on the slant of the potato agar test tube, and cultivate it at a constant temperature of 25°C for 13 days to grow snow...

Embodiment 2

[0032] (1) Acquisition of Ganoderma lucidum entities: In mid-August 2016, wild Ganoderma lucidum with good growth, no pests and diseases, and good color was collected as explants. Rinse with tap water 2-3 times, then wipe the ganoderma lucidum with absorbent cotton dipped in a 1.5% volume concentration of detergent solution, and set aside;

[0033] (2) Preparation of female species: with potato 200g, rice milk 20g, sugar 20g, agar 20g, water 100g as female seed culture medium, described rice milk is the product that rice and water beating obtain, and described rice and water weight ratio Put it into a test tube at a ratio of 1:3, put it into a test tube at a pressure of 103.4kPa and a temperature of 121.3°C, autoclave it for 18 minutes to prepare a potato agar test tube for use, and inoculate the collected wild Ganoderma lucidum fruiting body into the potato by the method of tissue separation The slant of the agar test tube can grow snow-white mycelium at a constant temperatur...

Embodiment 3

[0038] (1) Acquisition of Ganoderma lucidum entities: In late August 2016, wild Ganoderma lucidum with good growth, no pests and diseases, and good color was collected as explants. Rinse with tap water 2-3 times, then wipe the ganoderma lucidum with absorbent cotton dipped in a 1.5% volume concentration of detergent solution, and set aside;

[0039] (2) Preparation of female species: with potato 300g, rice milk 30g, sugar 30g, agar 30g, water 200g as female seed culture medium, described rice milk is the product that rice and water beating obtain, described rice and water weight ratio Put it into a test tube at a pressure of 103.4kPa and a temperature of 121.3°C at a ratio of 1:5, and autoclave for 20 minutes to prepare a potato agar test tube for use. The collected wild Ganoderma lucidum fruiting bodies are inoculated into potatoes by tissue separation. The slant of the agar test tube can grow snow-white mycelium at a constant temperature of 28°C for 18 days, and select high-...

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Abstract

The invention belongs to the technical field of asexual propagation of wild ganoderma, and in particular relates to a cultivation method of wild ganoderma. A cultivation method of wild Ganoderma lucidum, comprising the acquisition of Ganoderma lucidum entities, preparation of mother seeds, preparation of original seeds, cultivation of production species and cultivation of fruiting bodies of Ganoderma lucidum, wherein the raw materials of the mother seed culture medium include potatoes, rice milk, sugar, agar, and water; the original seeds The medium raw materials include sawdust, bellflower powder, wheat bran, wheat, corn, gypsum, sugar, calcium carbonate, and the balance is water; the production medium raw materials include sawdust, sweet potato powder, wheat, corn, soybean flour, sugar, and the balance It is water; the present invention uses wild ganoderma as a material to propagate strains indoors without seasonal restrictions, which can not only meet the quantity demand in production, but also maintain the traits of the female parent, and effectively select and breed high-quality ganoderma suitable for local development species, the large-scale production of Ganoderma lucidum industry has been realized, and it has good economic, social and ecological benefits.

Description

technical field [0001] The invention belongs to the technical field of asexual propagation of wild ganoderma, and in particular relates to a cultivation method of wild ganoderma. Background technique [0002] Ganoderma lucidum, also known as Lin Zhongling and Qiongzhen (scientific name: Ganoderma Lucidum Karst), is the fruiting body of polyporaceae fungus Ganoderma lucidum. Fujian, Guangdong, Guangxi and other provinces are distributed. Among them, the scale of Ganoderma lucidum planting in Longquan, Zhejiang, Anhui, and Tai'an, Shandong is relatively concentrated. At the same time, Ganoderma lucidum has a very wide range of applications. For the overall function of the human body, Ganoderma lucidum can regulate the function of the human body in two directions. It can regulate the balance of the function of the human body in two directions on the whole, so that the immune system of the human body can be rapidly improved comprehensively, and the internal organs and cells of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 赵志珩高中海廖健明曾广宇梁文汇黄晓露杨卓颖李宝财
Owner GUANGXI FORESTRY RES INST
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