Kit for quantitatively detecting content of pregnancy-related protein A

A technology for quantitative detection of pregnancy-related protein, applied in the field of kits for quantitative detection of pregnancy-related protein A content, can solve the problems of many influencing factors, many detection steps of ELISA method, and long time-consuming, so as to achieve reliable clinical reference value and reduce non-specific Heterotropic adsorption, good linear relationship effect

Inactive Publication Date: 2020-05-05
NINGBO AUCHEER BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ELISA method has many detection steps, takes a long time, and there are many influencing factors in the operation process, which can easily cause false positive and false negative results
Therefore, it is gradually replaced by the chemiluminescence method, but this method is a fully closed system, which is expensive and requires special training for instrument users. The maintenance and testing costs are high, and it is not suitable for single-person and small-batch testing. It is not conducive to PAPP at present. -A detection is

Method used

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  • Kit for quantitatively detecting content of pregnancy-related protein A
  • Kit for quantitatively detecting content of pregnancy-related protein A
  • Kit for quantitatively detecting content of pregnancy-related protein A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kit for quantitatively detecting the content of pregnancy-associated protein A, comprising a kit main body, a sealing cover, a protective cover, and a reagent. The kit main body is sequentially provided with solid-phase carrier holes, sample holes, detection antibody holes, fluorescein holes, washing Hole, reading hole, the inside of the solid phase carrier hole is a T-shaped structure, a protective cover is provided above the solid phase carrier hole, the protective cover and the solid phase carrier hole are connected by threads, and an internal thread is provided on the inner side of the lower end of the protective cover. The upper end of the carrier hole is provided with an external thread, the external thread matches the internal thread, a solid phase carrier is provided in the solid phase carrier hole, a serum sample is installed in the sample hole, and a biotin-labeled detection antibody is installed in the detection antibody hole , the fluorescein-labeled strept...

Embodiment 2

[0033] A kit for quantitatively detecting the content of pregnancy-associated protein A, comprising the steps of:

[0034] (1) Solid phase carrier coated with capture antibody: soak the lower end of the quartz needle in the polysaccharide complex for 1-3 minutes, freeze-dry at 4°C, and dilute the pregnancy-associated protein A capture antibody to 1mg with 0.01M PBS buffer / mL, place the lower end of the dried quartz needle in the pregnancy-associated protein A capture antibody solution, react for 2 hours at 24°C for labeling, wash 3 times with 0.1M PBS buffer after labeling, and then place in blocking solution Blocking for 8-12 hours, the blocking solution is Tris-HCL buffer containing 1% BSA, 0.1% casein, and 3% sucrose, and the solid phase carrier coated with the capture antibody is prepared;

[0035](2) Biotin-labeled detection antibody: Dilute the pregnancy-associated protein A detection antibody to 1mg / mL with 0.01M PBS buffer, and add biotin according to the mass ratio a...

Embodiment 3

[0038] 1. How to use the kit

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Abstract

The invention discloses a kit for quantitatively detecting the content of pregnancy-related protein A. The kit comprises a kit body, a sealing cover, a protective cover and a reagent, wherein a solidphase carrier hole, a sample hole, a detection antibody hole, a fluorescein hole, a washing hole and a reading hole are sequentially formed in the kit body; the kit contains all reagent components required for completing immunodetection; the end part of the solid phase carrier loaded in the kit is coated with a capture antibody, and the solid phase carrier is immersed into each hole in the kit sothat the combination of a target antigen in a detected sample and subsequent detection steps can be completed, and the manufacturing and maintenance costs are greatly reduced; the kit is high in detection result accuracy, good in linear relation and high in specificity, and reliable clinical reference value can be provided for early screening of Down's syndrome and early prediction of atherosclerotic plaque rupture.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a kit for quantitatively detecting the content of pregnancy-related protein A. Background technique [0002] Pregnancy-associated protein A (PAPP-A) is a macromolecular glycoprotein derived from the placenta. It is synthesized by placental syncytiotrophoblast cells, decidual cells and endometrial stromal cells in normal menstrual cycle and secreted into serum. Increases with increasing gestational age until full term. It has been proved that the concentration of PAPP-A in the serum of pregnant women with Down syndrome (DS) fetuses in the first trimester is significantly reduced, so PAPP-A is widely recognized as an effective indicator for DS screening, and its concentration reduction indicates that the newborn has Down syndrome. increased risk of Syndrome. [0003] At present, there are data showing that another form of PAPP-A protein is abundantly present in unstable coronary...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N33/552
CPCG01N33/552G01N33/582G01N33/6893G01N2333/471G01N2800/323G01N2800/387
Inventor 周义正董文霞唐静黄丹娣俞辰泽余城滨
Owner NINGBO AUCHEER BIOTECHNOLOGY CO LTD
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