Novel goose arbovirus detection primer group and kit

Abstract

The invention discloses real-time fluorescence quantification PCR (polymerase chain reaction) detection primers for a novel goose arbovirus which causes gout of goslings, and a kit of the primers. Thekit comprises an upstream primer GoAstV-F1 and a downstream primer GoAstV-R1. The kit disclosed by the invention is capable of detecting the novel goose arbovirus which causes gout of the goslings, has the advantages of being good in specificity, good in repeatability and accurate in quantification, is good in sensitivity, is capable of detecting 6.8 copies at least, has a very good linear relationship within a 6.88-6.88*10<9> copy range of a standard product, has a detection range up to 10 orders of magnitudes, is capable of rapidly detecting the novel goose arbovirus GoAstV which causes gout of the goslings, and improves the detection efficiency.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to a real-time fluorescent quantitative PCR detection primer and a kit thereof for a novel goose astrovirus. Background technique [0002] Astrovirus (Arbovirus) is a single-stranded positive-sense RNA virus belonging to the family Astroviridae. The genome length is 6.1-7.9kb, and the genome includes 5'UTR, ORF1a, ORF1b and ORF2 three open reading frames and 3'URT , polyadenylic acid (PolyA) tail. The virus infection has a certain prevalence, and can cause enteritis and diarrhea in humans or animals, accompanied by vomiting and diarrhea. Astroviridae can be divided into mammalian genera and avian genera according to different hosts, among which mammals are divided into human astrovirus, feline astrovirus, porcine astrovirus, sheep astrovirus and mink astrovirus according to different hosts. Astroviruses; the genus Astrovirus includes duck Astrovirus (DAstV), turkey Astrov...

AI Technical Summary

Problems solved by technology

Regarding the diagnosis of the virus, the traditional virus isolation and identification is time-consuming and laborious, and is not suitable for clinical use. Moreover, the virus has no hemagglutination, does not grow on chicken embryos, duck embryos, and the reproductive titer on goose embryos is not very high.

At present, the most common method is to detect GoAstV by ordinary PT-PCR method, which is easy to cause cross-contamination between samples, and requires gel electrophoresis for judgment, and is not suitable for detection of a large number of clinical samples.

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