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Preparation method of wood-based bionic bone scaffold material

A scaffold material and bionic bone-based technology, applied in the field of bionic bone scaffolds, to achieve the effect of high three-dimensional porous structure, no cytotoxicity, and good bone cell compatibility

Active Publication Date: 2020-04-28
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects of existing bone scaffold materials, the present invention provides a preparation method of a wood-based bionic bone scaffold material, which uses the mechanical properties and biocompatibility of lignocellulose and the osteoconductivity of hydroxyapatite to obtain a wood-based bionic bone scaffold material. Biomimetic Bone Scaffold Materials

Method used

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  • Preparation method of wood-based bionic bone scaffold material
  • Preparation method of wood-based bionic bone scaffold material
  • Preparation method of wood-based bionic bone scaffold material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Soak the wood fiber in the alkali solution prepared with 0.01mol / L sodium hydroxide solution and 0.01mol / L sodium sulfite solution according to the mass ratio of 1:1, and then perform mechanical grinding at a speed of 2800r / min Treat for 2 hours to prepare fiber pulp with a mass fraction of 10%;

[0050](2) In a water bath at 40°C, chitosan is dissolved in pure water to prepare a chitosan polymer solution with a mass fraction of 10%, and the fiber pulp with a mass fraction of 10% obtained in step 1 and 10% mass fraction The chitosan polymer solution was mixed at a mass ratio of 1:1, stirred evenly, and kept magnetically stirred in a water bath at 40°C for 0.5h to obtain a mixed solution;

[0051] (3) Add hydroxyapatite to the mixed solution obtained in step 2, and carry out magnetic stirring at 40°C in a water bath for 0.5h to obtain a scaffold material precursor mixed solution, wherein the amount of hydroxyapatite added is the same as that of chitosan and wood The...

Embodiment 2

[0056] (1) Soak the wood fiber in the alkali solution prepared with 0.01mol / L sodium hydroxide solution and 0.01mol / L sodium sulfite solution according to the mass ratio of 1:2, and then perform mechanical grinding at a speed of 2800r / min Treat for 2 hours to prepare fiber pulp with a mass fraction of 10%;

[0057] (2) In a water bath at 50°C, chitosan is dissolved in pure water to prepare a mass fraction of 10% chitosan macromolecule solution, and the fiber pulp with a mass fraction of 10% obtained in step 1 and 10% of the mass fraction The chitosan polymer solution was mixed and stirred evenly at a mass ratio of 1:2, and kept magnetically stirred in a water bath at 50°C for 0.5h to obtain a mixed solution;

[0058] (3) Add hydroxyapatite to the mixed solution obtained in step 2, and carry out magnetic stirring at 40°C in a water bath for 0.5h to obtain a scaffold material precursor mixed solution, wherein the amount of hydroxyapatite added is the same as that of chitosan and...

Embodiment 3

[0063] (1) Soak the wood fiber in the alkali solution prepared according to the mass ratio of 2:1 0.01mol / L sodium hydroxide solution and 0.01mol / L sodium sulfite solution, and then perform mechanical grinding at a speed of 2800r / min Treat for 2 hours to prepare fiber pulp with a mass fraction of 20%;

[0064] (2) In a water bath at 60°C, chitosan is dissolved in pure water to prepare a chitosan polymer solution with a mass fraction of 20%, and the fiber pulp with a mass fraction of 20% obtained in step 1 and 20% of the mass fraction The chitosan polymer solution was mixed and stirred evenly at a mass ratio of 1:1, and kept magnetically stirred in a water bath at 60°C for 0.5h to obtain a mixed solution;

[0065] (3) Add hydroxyapatite to the mixed solution obtained in step 2, and stir magnetically at 60° C. in a water bath for 0.5 h to obtain a scaffold material precursor mixed solution, wherein the amount of hydroxyapatite added is the same as that of chitosan and wood fiber...

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Abstract

The invention relates to a preparation method of a wood-based bionic bone scaffold material, and belongs to the field of bionic bone scaffolds. The method comprises the following steps: soaking wood fibers in an alkali solution, and performing mechanical grinding to obtain fiber pulp; mixing the fiber pulp with a polysaccharide polymer solution, and performing uniform stirring to obtain a mixed solution; adding hydroxyapatite into the mixed solution, and performing magnetic stirring in a water bath to obtain a scaffold material precursor mixed solution; freezing the scaffold material precursormixed solution, and performing freeze-drying to obtain an aerogel scaffold; performing cross-linking treatment on the aerogel scaffold with a glutaraldehyde solution, and performing rinsing with ethanol and deionized water; and freezing the rinsed scaffold material, and then performing freeze-drying to obtain the wood-based bionic bone scaffold material. According to the invention, the wood-basedbionic bone scaffold material with a three-dimensional porous structure is prepared by a circulating freeze drying technology. The scaffold material has no cytotoxicity and has high compressive strength; and mouse osteoblasts can adhere and grow on the scaffold material, so that requirements of a bone scaffold material are met.

Description

technical field [0001] The invention relates to a preparation method of a wood-based bionic bone support material, belonging to the technical field of bionic bone support. Background technique [0002] Bone tissue is composed of ordered collagen, oriented hydroxyapatite and water. Bone tissue closely combines the organic bone matrix structure and the inorganic bone salt framework structure to form an inorganic / organic composite material, thereby meeting the functional requirements of biology and mechanics. When the bone tissue is removed due to congenital, traumatic and tumor deformities, a large area of ​​bone defect is formed. It cannot heal through its own repair ability, so it is necessary to fill the defect with suitable bone repair materials through bone grafting to facilitate the healing and growth of bone tissue. [0003] Hydroxyapatite is the main component of inorganic bone salt and has good biocompatibility, bioactivity and osteoconductivity. Nano-scale hydroxy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/56A61L31/12A61L31/14
CPCA61L27/56A61L31/146A61L27/46A61L31/127A61L2430/02A61L2400/12C08L1/00
Inventor 孙庆丰党宝康王超陈逸鹏
Owner ZHEJIANG FORESTRY UNIVERSITY
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