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Stabilizer of enterobacter aerogenes and application thereof

A technology of Enterobacter aerogenes and stabilizer, which is applied in the field of microbial quality control to achieve the effect of maintaining stability

Active Publication Date: 2020-04-14
GUANGDONG HUANKAI BIOLOGICAL SCI & TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no preparation method for the quantitative quality control strain of Enterobacter aerogenes. The key reason is that there is no stabilizer that can prevent the loss of Enterobacter aerogenes during the freeze-drying process, and improve the long-term storage of Enterobacter aerogenes. Stability during short-term transportation

Method used

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  • Stabilizer of enterobacter aerogenes and application thereof
  • Stabilizer of enterobacter aerogenes and application thereof
  • Stabilizer of enterobacter aerogenes and application thereof

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Embodiment Construction

[0030] In order to make the object, technical solution and technical effect of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific implementations described in this specification are only for explaining the present invention, not for limiting the present invention.

[0031] For the convenience of comparison, the operations of strain recovery, enrichment, counting and freeze-drying are as follows:

[0032] strain recovery

[0033] The standard strain of Enterobacter aerogenes was streaked and inoculated into TSA medium for recovery culture at 36±1°C and identified.

[0034] Bacteria

[0035] The resuscitated and identified strains were inoculated into the Enterobacter aerogenes proliferation medium, and cultured in a constant temperature shaker at 36° C. with a rotation speed of 150 rpm / min for 8 to 12 hours.

[0036] count

[0037] Draw an appropri...

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PUM

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Abstract

The invention provides a stabilizer of enterobacter aerogenes. The stabilizer comprises the following raw materials: L-carnosine, polyethylene glycol, skim milk powder, glycine and ascorbic acid. Thestabilizer of the enterobacter aerogenes quality control bacteria can make the freeze-drying survival rate of the quality control bacteria reach 95% or above, and also can keep the bacterial content stability in the long-term storage process.

Description

technical field [0001] The invention relates to the field of microbial quality control, in particular to a stabilizer for Enterobacter aerogenes and its application. Background technique [0002] Vacuum freeze-drying technology was developed in the early 1970s and is now commercially produced. Freeze-drying is to pre-freeze the bacteria below the eutectic point, and then sublimate the ice in the bacteria under a high vacuum state lower than the triple point pressure, and finally achieve the purpose of drying. However, in the process of freeze-drying, it is easy to cause damage and death of some microbial cells and passivation of some enzyme protein molecules. Many studies have found that adding appropriate substances before bacterial freeze-drying can play a protective role, that is, the use of effective protective agents Freeze-drying of bacteria is the key to success. Adding a protective agent during the freeze-drying process can reduce or avoid the damage to cells cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12Q1/10C12Q1/06C12R1/01
CPCC12N1/20C12N1/04C12Q1/10C12Q1/06G01N2333/265Y02A50/30
Inventor 陈惠元蔡芷荷徐环卢勉飞吴清平林国强张建明
Owner GUANGDONG HUANKAI BIOLOGICAL SCI & TECH CO LTD
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