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Method for producing yeast cycloastragenol polypeptide mixture and its anti-aging application

A technology of cycloastragalus and mixed solution, which is applied in the fields of biotechnology and cosmetics, can solve the problems of non-compliance with safety requirements, easy contamination of microorganisms, long time period, etc., and achieves diluting static and dynamic lines of epidermis, supplementing nutrient elements, delaying Effects of skin aging

Active Publication Date: 2022-03-29
武汉克鲁金生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microorganisms used in the reported patents are often genetically engineered bacteria such as Escherichia coli, which not only do not meet the safety requirements of food and cosmetics, but also involve safety issues such as genetic modification; Low efficiency, long time period, and easy to contaminate microorganisms in the production process

Method used

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  • Method for producing yeast cycloastragenol polypeptide mixture and its anti-aging application
  • Method for producing yeast cycloastragenol polypeptide mixture and its anti-aging application
  • Method for producing yeast cycloastragenol polypeptide mixture and its anti-aging application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The fermentation process of embodiment 1 Kluyveromyces lactis XT82

[0033] The preserved Kluyveromyces lactis (Kluyveromyces lactis) XT82 was inoculated into 3-10ml YPD liquid medium, and the temperature was 30°C, and the rotation speed was 250rpm for continuous cultivation for 48 hours; Received in 1-10L YPD liquid culture medium, cultured continuously for 48-72 hours at a temperature of 30°C and a rotation speed of 250rpm; after the cultivation, centrifuged at a rotation speed of 3000-8000rpm for 10-30min to collect the cells; , YPD liquid medium:

[0034] 10 grams of yeast extract and 20 grams of peptone in 900ml of deionized water, sterilized at 121°C for 20 minutes; after cooling to about 60°C, add 50ml of filtered sterilized 40% glucose solution, and mix well.

Embodiment 2

[0035] Example 2 Preparation of Crude Enzyme Solution

[0036] The cells collected in Example 1 were washed 2-3 times with deionized water. Add 0.05-0.5 L of deionized water to suspend the cells, and place them in a high-pressure homogenizer at a pressure of 1500-2000 bar to break the cells. Incubate the broken cells at 95°C for 30 minutes, and after the proteins with intolerance to high temperature are completely denatured, centrifuge at 5,000-10,000 rpm for 30 minutes, collect the supernatant, which is the crude enzyme solution, and store at 4°C.

Embodiment 3

[0037] Example 3 Production method of yeast cycloastragenol polypeptide mixture 1

[0038] 1) Take 1 L of the enzyme solution in Example 2, add 40 grams of Astragalus extract containing 5% astragaloside IV, stir and react at a temperature of 70-80° C. for 1-3 hours; obtain a reaction liquid;

[0039] 2) Then add 15-30 times of water to the reaction liquid to dilute, then cool down to 25-40°C, and then add carnosine (Cas: 305-84-0) and palmitic acid pentapeptide (Cas: 214047-00-4) in sequence and acetyl hexapeptide-8 (Cas: 616204-22-9); obtain yeast cycloastragenol polypeptide mixture; store at -20°C;

[0040] Wherein, in the yeast cycloastragenol polypeptide mixture, the content of cycloastragenol is 0.01%,

[0041] The content of carnosine is 100ppm,

[0042] The content of palmitic acid pentapeptide is 50ppm,

[0043] The content of acetyl hexapeptide-8 was 50 ppm.

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Abstract

The invention discloses a method for producing yeast cycloastragenol polypeptide mixed liquid and its anti-aging application. In the method, astragalus extract and crude enzyme liquid of Kluyveromyces lactis mutant strain XT82 are stirred and reacted to obtain a reaction liquid; and then Dilute the reaction liquid with water, then lower the temperature to 20-60°C, and then add carnosine, palmitic acid pentapeptide and acetyl hexapeptide-8 in sequence; obtain the yeast cycloastragenol polypeptide mixture; the yeast cycloastragenol polypeptide mixture in Application in the preparation of anti-aging products.

Description

technical field [0001] The invention relates to the fields of biotechnology and cosmetics, in particular to a method for producing yeast cycloastragenol polypeptide mixture and its anti-aging application. Background technique [0002] As a traditional Chinese medicine, Astragalus has been used to this day because of its functions of invigorating qi and invigorating the spleen, raising yang and lifting depression, benefiting the body and strengthening the exterior. Cycloastragenol is a cyclopine-type tetracyclic triterpenoid compound, which mainly exists in Astragalus membranaceus in the form of aglycone in nature. It is reported that it is mainly prepared from the index component of Astragalus membranaceus --- Astragaloside IV. In 2010, GERON Biotechnology Company of the United States found that cycloastragenol exerted its biological effect mainly by activating telomerase. This discovery actively promoted the research work of cycloastragenol. Granzyme-related anti-aging enh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61K8/64A61Q19/08
CPCA61K8/9789A61K8/64A61Q19/08A61K2800/85A61K2800/78
Inventor 胡莎莉刘建蔚方德明王安华
Owner 武汉克鲁金生物科技有限公司
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