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Brevibacillus laterosporus MES818, and microbial agent and preparation method and application thereof

A technology of MES818 and Bacillus brevius, applied in the direction of botany equipment and methods, biochemical equipment and methods, applications, etc., can solve problems such as failure to achieve, accumulation of toxic substances, pollution of the environment, etc., to promote the improvement of yield and quality, Prevents ginseng rust and rot, and is easy to use on a large area

Inactive Publication Date: 2020-04-07
天津开发区坤禾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional prevention and control of rust and rot mostly adopts chemical control, which not only fails to achieve the expected effect, but also seriously pollutes the environment, destroys the soil environment, causes the accumulation of toxic substances, and ultimately affects the yield and quality of ginseng

Method used

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  • Brevibacillus laterosporus MES818, and microbial agent and preparation method and application thereof
  • Brevibacillus laterosporus MES818, and microbial agent and preparation method and application thereof
  • Brevibacillus laterosporus MES818, and microbial agent and preparation method and application thereof

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preparation example Construction

[0043] The preparation method of the bacterial agent provided by the present invention has simple process and convenient operation, and is suitable for large-scale expansion and cultivation.

[0044] In some preferred embodiments, the fermentation is terminated when the sporulation rate is greater than 90%.

[0045] When the spore formation rate is greater than 90%, the fermentation efficiency is the highest, avoiding waste of resources, and also avoiding the decline of bacteria caused by over-fermentation.

[0046] Specifically, the preparation method of the above bacterial agent comprises the following steps:

[0047] ① Shake flask culture: The strain activated on the slant was subjected to seed shake flask culture. Shake flask medium is: beef extract peptone medium (soy peptone 8-10 grams, beef extract 2-4 grams, sodium chloride 4-5 grams, agar 18-20 grams, ultrapure water 1000 ml, pH value 7.0 -7.5). The temperature is 28°C-32°C, under the condition of 180-220rpm, shake...

Embodiment 1

[0056] The isolation and screening of embodiment 1 bacterial strain

[0057] The strain of Brevibacillus sporogenes MES818 was isolated from the root soil of kiwifruit by our R&D personnel in June 2018. After weighing the soil sample, dilute it in a gradient with sterile water, culture it at 28°C for 24 hours by streaking on the plate, pick a single colony to make a slant strain, and refrigerate at 4°C. A single colony was picked for microscopic examination, and was later identified as Brevibacillus lateralosporus by the strain detection agency of the Ministry of Agriculture. Taking the isolated and preserved ginseng rust rot fungus Cylindrocarpon destructans (Zinss.) Scholtan as the target, the plate confrontation experiment was carried out with Brevibacillus lateralosporus, and cultured at 28°C for 7 days. It was found that Brevibacillus lateralsporosa could Significantly inhibit the growth of pathogenic bacteria, and a wide inhibition zone appears, so it is named MES818. ...

Embodiment 2

[0058] The preparation of embodiment 2 MES818 microbial inoculum

[0059] Follow the steps below:

[0060] ① Shake flask culture: The strain activated on the slant was subjected to seed shake flask culture. Shake flask medium is: beef extract peptone medium (soy peptone 8-10 grams, beef extract 2-4 grams, sodium chloride 4-5 grams, agar 18-20 grams, ultrapure water 1000 ml, pH value 7.0 -7.5). The temperature is 28°C-32°C, under the condition of 180-220rpm, shake culture for 18-24h.

[0061] ②First-level seed culture: the strain MES818 activated on the test tube slant was cultured in Anbei bottle, and the medium in the Anbei bottle was broth medium. The formula of the culture medium is: 8-10 grams of soybean peptone, 2-4 grams of beef extract, 4-5 grams of sodium chloride, 18-20 grams of agar, 1000 milliliters of ultrapure water, and the pH value is 7.0-7.5. The culture conditions are: temperature 28°C, culture 24h.

[0062] ③ Fermentation: at 30m 3 A 20L liquid Brevibac...

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Abstract

The invention, which relates to the technical field of microorganisms, provides brevibacillus laterosporus MES818, and a microbial agent and a preparation method and application thereof. The brevibacillus laterosporus MES818 is deposited with the China General Microbiological Culture Collection Center on December 17th, 2018 and is assigned the accession number CGMCC NO.16859.The brevibacillus laterosporus MES818 has a strong inhibition effect on pathogenic bacteria of the ginseng rust rot and can prevent the ginseng rust rot; and the brevibacillus laterosporus MES818 is used for preventing andtreating the ginseng rust rot to promote the improvement of the yield and quality of ginseng.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Brevibacillus spp. MES818, a bacterial agent, a preparation method and an application. Background technique [0002] Ginseng rust rot is a soil-borne disease, and it is one of the most common and harmful diseases of ginseng. It can infect the entire growth period from early spring to late autumn, and has a great impact on the yield and quality of ginseng. The annual incidence rate is 20% to 30% %, the incidence rate of severe plots is as high as 80% to 100%. When the disease is mild, the epidermis is intact, and only a few layers of cells under the epidermis are infected, and the inner tissue of the root is not infected; when the disease is severe, it not only destroys the epidermis, but also penetrates into the root tissue, and a large amount of rust powder accumulates in the lesion, showing dry rot. or taproot rots laterally. The aboveground part shows that the plant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 肖培英顾欣燕闫辉敏赵晓琛
Owner 天津开发区坤禾生物技术有限公司
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