Composition containing recombinant human collagen, freeze-dried material and preparation method thereof, and cosmetics
A technology of human collagen, composition, applied in the field of cosmetics
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Embodiment 1
[0066] A composition containing recombinant human collagen, the raw materials of which are expressed in mass percent, comprising: 1% mannitol, 1.5% pullulanase polysaccharide, 0.7% recombinant human collagen, 0.05% mevalonolactone and water 96.75%.
[0067] Mix the above raw materials evenly, sterilize, pass through a 400-mesh filter cloth, sub-package, and freeze-dry to obtain freeze-dried powder.
Embodiment 2
[0069] A composition containing recombinant human collagen, the raw materials of which are expressed in mass percent, including: 1% mannitol, 1.5% pullulanase polysaccharide, 0.1% sodium hyaluronate, 0.7% recombinant human collagen, Centella asiatica Extract 0.5%, Mevalonolactone 0.05% and Water 96.15%.
[0070] Mix mannitol, pullulanase polysaccharide and ultrapure water, stir until the solution is clear and transparent, filter it with a 400-mesh filter cloth and put it in a high-temperature steam sterilizer for high-temperature sterilization (121°C, 0.1Mpa, 30 minutes) Obtain A solution;
[0071] Stir sodium hyaluronate, recombinant human collagen and centella asiatica extract with sterile warm water (31°C) until completely dissolved, then add mevalonolactone, stir evenly, and filter with a 500-mesh filter cloth to obtain solution B ;
[0072] After the A solution is lowered to 25°C, filter it with a 400-mesh filter cloth, add the B solution, stir evenly, and immediately d...
Embodiment 3
[0079] A composition containing recombinant human collagen, the raw materials of which are calculated by mass percentage: 5% mannitol, 1.5% pullulanase polysaccharide, 0.01% sodium hyaluronate, 0.7% recombinant human collagen, centella asiatica Extract 0.5%, Mevalonolactone 0.05% and Water 92.24%.
[0080] Mix mannitol, pullulanase polysaccharide and ultrapure water, stir until the solution is clear and transparent, filter it with a 500-mesh filter cloth, and put it into a high-pressure steam sterilizer for high-temperature sterilization (121°C, 0.1Mpa, 30 minutes) Obtain A solution;
[0081]Stir sodium hyaluronate, recombinant human collagen and centella asiatica extract with sterile warm water (35°C) until completely dissolved, then add mevalonolactone, stir evenly, and filter with a 500-mesh filter cloth to obtain solution B ;
[0082] After the A solution is lowered to normal temperature, filter it with a 500-mesh filter cloth, add the B solution, stir evenly, and immedi...
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