Application of nocardia immunopotentiator in preparation of poultry vaccines

An immune enhancer, the technology of Nocardia, applied in the field of microorganisms, can solve the problems of no obvious promotion effect of immune effect, no obvious promotion effect, narrow application range, etc., and achieve low cost, high safety and wide application range. Effect

Pending Publication Date: 2020-03-31
广州科力生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this promotion is aimed at specific macrophages, and the scope of application is narrow, and the range of strains that can be selected is also narrow, and there is no obvious promotion effect on the immune effect of animals
[0006] In summary, the immunopotentiators in the prior art generally have the disadvantages of poor immune effect, narrow scope of application, and inconspicuous promoting effect.

Method used

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  • Application of nocardia immunopotentiator in preparation of poultry vaccines
  • Application of nocardia immunopotentiator in preparation of poultry vaccines
  • Application of nocardia immunopotentiator in preparation of poultry vaccines

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Embodiment 1

[0031] Embodiment 1 A kind of preparation process of Nocardia immunopotentiator

[0032] The Nocardia immunopotentiator is prepared by using Nocardia, and the preparation method is as follows:

[0033] Nocardia was cultured in a 30L fermenter for 48 hours, and then inactivated at 121°C and 101kPa under high temperature and pressure for 20 minutes to obtain the product;

[0034] The formula of the medium in the fermenter is 2% glucose, 1% yeast powder, 2% tryptone, 95% purified water, pH7.0-7.2;

[0035] The culture conditions in the fermenter are: stirring speed 310r / min, ventilation rate 1:1.0, inoculum size 8%, liquid filling volume 65% of tank volume, temperature 30°C.

Embodiment 2

[0036] The preparation of embodiment 2 poultry oil emulsion vaccine

[0037] I. Sterilization and quantification of Nocardia adjuvant samples

[0038] A number of Nocardia adjuvant liquid samples were taken and inactivated by high-pressure steam at 121°C for 30 minutes. After sterilization, the samples were stored at 4°C until the temperature dropped to room temperature.

[0039] Adjuvant samples need to be quantified before use. The quantification method is as follows: Aseptically pipette 2 mL of adjuvant samples and divide them equally into two 1.5 mL eppendorf tubes, centrifuge at 12000 rpm / min for 5 min at high speed, discard the supernatant, and place them in a drying oven at 103 °C for 2 hours to fully dry the samples. dry matter weight.

[0040] II. Preparation of oil emulsion inactivated vaccine with Nocardia adjuvant added

[0041] ⑴Preparation of Newcastle disease+H9 subtype avian influenza dual oil emulsion inactivated vaccine

[0042] ①Preparation of antigen aq...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to an application of a nocardia immunopotentiator in preparation of poultry vaccines. An immunologic adjuvant is prepared by culturing nocardia through a fermentation tank, sterilizing at high pressure and extracting. The nocardia culture or fermentation product is used as the immunologic adjuvant for preparing the poultry vaccine, so that the nocardia vaccine has the advantages of high safety, no residue in vaccine injection and capability of remarkably improving the immunity of animals.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to the application of a Nocardia immune enhancer in the preparation of poultry vaccines. Background technique [0002] Immunopotentiators refer to substances that can enhance the body’s immune response either alone or simultaneously with antigens. There are many varieties of adjuvants, which can be divided into three categories according to their prerequisites: (1) Immune substitutes, used to replace certain Drugs with biological factors that enhance immunity; (2) immune recovery agents, which can enhance the suppressed immune function, but have little effect on normal immune function; (3) immune adjuvants, also known as non-specific stimulators. Commonly used immune enhancers include BCG, Corynebacterium pumilus, endotoxin, immune ribonucleic acid, thymosin, transfer factor, double-stranded polynucleotide, aluminum hydroxide adjuvant and so on. [0003] Although some ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/12A61P37/04A61P31/12
CPCA61K39/39A61K39/12A61P37/04A61P31/12A61K2039/55594A61K2039/552A61K2039/5252
Inventor 王文勇严悌昆张毓金
Owner 广州科力生物科技有限公司
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