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Bivalent subunit vaccine of Newcastle disease and infectious bursal disease

A technology for bursal disease and Newcastle disease virus, which is applied in the field of dual subunit vaccines for Newcastle disease and infectious bursal disease, can solve the problems of virus pollution, foreign virus pollution, cumbersome process, etc., and save production costs , convenient and quick cost, and the effect of ensuring safety

Pending Publication Date: 2020-03-27
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing ND and IBD vaccines have problems such as foreign virus contamination, antigenic mismatch, poor immune effect and cumbersome process
In addition, commercial live vaccines are frequently used in poultry flocks, resulting in serious virus contamination in some areas, which is not conducive to the purification of the disease

Method used

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  • Bivalent subunit vaccine of Newcastle disease and infectious bursal disease
  • Bivalent subunit vaccine of Newcastle disease and infectious bursal disease
  • Bivalent subunit vaccine of Newcastle disease and infectious bursal disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] (1) Preparation of virus-like particles

[0020] 1. Construction of recombinant baculovirus rBV-NDV-HN+F+M

[0021] The current epidemic type VII NDV virulent NA-1 strain (GenBank accession number: DQ659677. Ding Zhuang, et al. New characteristics of Newcastle disease epidemiology and goose Newcastle disease prevention and control strategy. Chinese Journal of Veterinary Medicine, 2015, 35(1): 38-40.) M, F, HN gene sequence information, artificially synthesized nucleic acid sequences optimized by insect cell codons (synthesized by General Biosystems (Anhui) Co., Ltd.), the nucleotide fragments between each gene are connected in series, NDV-HN+F+M is formed by linking with T2A cut peptide, and its nucleotide sequence is SEQ ID NO:1. EcoR I and Not I restriction sites were introduced at both ends of its artificially synthesized sequence. Subsequently, the synthetic sequence was combined with the shuttle plasmid pFastBac1 TM (product of Thermo Fisher Scientific Co., Ltd....

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Abstract

The invention discloses a bivalent subunit vaccine of Newcastle disease and infectious bursal disease, and belongs to the field of veterinary biological products. The subunit vaccine is prepared by taking Newcastle disease virus-like particles and infectious bursal disease virus-like particles as antigens, infecting insect cells by recombinant baculoviruses (rBV-NDV-HN+F+M and rBV-IBDV-doubleVP2),and performing purifying and inactivating. The subunit vaccine provided by the invention can rapidly generate specific antibodies, and can be used for preventing infection of Newcastle disease virusand infectious bursal disease virus. Experiments show that the subunit vaccine provided by the invention has better immune protection efficacy compared with commercial vaccines in preventing infectionof a Newcastle disease virus virulent strain and infection of infectious bursal disease virus.

Description

technical field [0001] The invention discloses a dual subunit vaccine of Newcastle disease and infectious bursal disease, which belongs to the field of veterinary biological products. Background technique [0002] Newcastle disease (ND) is an acute and highly contagious poultry infectious disease caused by a virulent strain of Newcastle disease virus (NDV). One of a class of animal diseases. Infectious bursal disease (IBD) is an immunosuppressive infectious disease caused by infectious bursal disease virus (IBDV), which is mainly characterized by invading poultry bursa, and is a major disease that endangers the chicken industry. In recent years, although the widespread use of ND and IBD vaccines has significantly reduced the outbreak of the disease, new epidemic features such as atypical symptoms, immune transmission, immune failure, regional epidemics, and mixed infections have emerged, which directly and indirectly caused The economic loss is huge, and prevention and con...

Claims

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Application Information

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IPC IPC(8): A61K39/17A61K39/12A61K39/39A61P31/14
CPCA61K39/12A61K39/39A61P31/14A61K2039/552A61K2039/5252A61K2039/70A61K2039/55505C12N2760/18134C12N2720/10034
Inventor 钱晶王永山欧阳伟马孙婷王晓丽夏兴霞王晶宇诸玉梅
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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