Preparation method for mouse thrombotic stroke model

Abstract

The invention provides a preparation method for a mouse thrombotic stroke model. The method comprises the following steps: after anesthetizing a mouse, making a slipknot on a common carotid artery byusing a suture line, and separating and permanently ligating an external carotid artery and a pterygopalatine artery; separating the initial part of an internal carotid artery; putting the common carotid artery into a groove of a blood vessel electric shock clip of a thrombus generator, and carrying out electrical stimulation to form a thrombus; taking off the electric shock clip and clipping thedistal end of the common carotid artery; crushing the thrombus by using a pair of microscope forceps, enabling the artery to pulsate again, and impacting and crushing the thrombus; releasing an arteryclip; after expelling the thrombus to enter the internal carotid artery by using the forceps, immediately closing the proximal end of the common carotid artery; and opening all artery clips after pre-set time, so as to form a target thrombus model. The mouse thrombotic stroke model provided by the invention can be applied to researches of treatment medicines and methods for stroke, and pathogenesis.

Description

technical field [0001] The present invention relates to a preparation method of an animal cerebral apoplexy model, in particular to a preparation method of a novel mouse thrombotic cerebral apoplexy model, and the preparation method of the prepared mouse thrombotic cerebral apoplexy model in the dissolution of cerebral thrombus. Applications in Thrombosis Research. Background technique [0002] Stroke, also known as stroke or cerebrovascular accident, is a common and frequently-occurring disease that seriously threatens human health. The latest data show that about 2.7 million new strokes occur in my country every year, and the rate of increase is nearly 9% per year. It is the number one cause of death and disability among adult residents in my country. Stroke is divided into ischemic and hemorrhagic, among which ischemic stroke is the most common type of stroke in clinical practice (accounting for about 80% of the incidence rate), which is mainly caused by various emboli i...

AI Technical Summary

Problems solved by technology

(2) Many molecular, genetic, biochemical or pathological examinations require a certain amount of brain tissue, which is extremely difficult to achieve in clinical operations

(3) The pathological changes in the very early stage of stroke often cannot be observed by clinical imaging techniques, so they can only be studied by animal models

(4) It is difficult to simulate the vascular structure and the pathological process of reperfusion in animal models using in vitro cell models

To sum up, there are two main problems: it does not conform to the actual clinical pathological process and the study of the time window of thrombolysis cannot be carried out.

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