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Ganoderma lucidum sporocarp microbial fermentation preparation for treating intestinal flora disorder and intestinal barrier function impairment

A technology of Ganoderma lucidum fruiting body and microbial fermentation, applied in the field of fermentation engineering, to improve the effect of impaired intestinal barrier function

Inactive Publication Date: 2020-03-17
合肥康春堂药业有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] How to rationally use the co-fermentation of Ganoderma lucidum fruiting body and Bacillus subtilis to prepare a microbial fermentation preparation of Ganoderma lucidum fruiting body for the treatment of intestinal flora disorder and impaired intestinal barrier function is a topic worthy of further research, and there is no such thing as yet. Related literature reports

Method used

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  • Ganoderma lucidum sporocarp microbial fermentation preparation for treating intestinal flora disorder and intestinal barrier function impairment
  • Ganoderma lucidum sporocarp microbial fermentation preparation for treating intestinal flora disorder and intestinal barrier function impairment
  • Ganoderma lucidum sporocarp microbial fermentation preparation for treating intestinal flora disorder and intestinal barrier function impairment

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Effect test

Embodiment 1

[0034] A preparation method of ganoderma lucidum fruiting body microbial fermentation preparation for treating intestinal flora disorder and impaired intestinal barrier function, the steps are as follows:

[0035] 1) Screening of strains: Screen the strains that can grow in Ganoderma lucidum medium from the following probiotic strains, including Bacillus subtilis, Lactobacillus acidophilus, Bifidobacterium breve, Lactobacillus casei, Pseudomonas pseudobacterium Bifidobacterium streptoides and Lactobacillus gasseri.

[0036] 2), preparation of MRS medium containing three concentrations of Ganoderma lucidum extract in low, medium and high concentrations

[0037] Ganoderma lucidum fruiting body powder was prepared with water into Ganoderma lucidum extracts with three concentrations of 8.8mg / ml, 16.6mg / ml, and 33.2mg / ml, and then added to MRS medium containing 0.5% sugar respectively, and processed under high pressure to produce MRS medium containing Ganoderma lucidum extract.

...

Embodiment 2

[0042] Determination of the growth of probiotics and the content of total sugar in probiotic fermentation broth of Ganoderma lucidum fruiting bodies.

[0043] Microbial growth: Measure the OD value at 620nm and draw the growth curve to obtain the growth of probiotics.

[0044] Determination of total sugar content: Glucose is the standard product, using the phenol-concentrated sulfuric acid method, take 6 clean test tubes and number them, draw 0, 0.2, 0.4, 0.6, 0.8, 1.0ml of 0.1mg / ml standard glucose solution into the test tubes respectively, Make up to 1ml with distilled water, add 1ml of 6% phenol and 5ml of concentrated sulfuric acid to each test tube, mix well and let stand for 10min. React in a water bath at 30°C for 20 minutes, take an appropriate amount of the reaction solution and measure the absorbance at 490 nm, draw a standard curve with glucose concentration (ug / ml) as the abscissa and absorbance as the ordinate. Take 2ml of fermentation broth, add 5ml of water, 1....

Embodiment 3

[0047] Application of Ganoderma lucidum fruiting body-Bacillus subtilis fermentation liquid (obtained by inoculation and fermentation of Bacillus subtilis (such as DM9610, etc.), the preparation method is the same as that in Example 1) in animal models of intestinal flora disturbance and impaired intestinal barrier function.

[0048] Mainly observe the changes in animal weight, feces, and cecal index; pathological detection of colon tissue HE staining; flow cytometry analysis of spleen CD3 + T cells, CD4 + T cells, CD8 + Changes of T cells and macrophages; ELISA double-antibody sandwich method to detect IL-10, IL-6, TNF-α, LPS; 16SrDNA high-throughput sequencing to detect intestinal flora. The specific steps are:

[0049]Male Balb / c mice aged 6-8 weeks were used to construct animal models and divided into 5 groups (7 mice in each group), which were control (CON) group, antibiotic (CS) group, fermentation broth treatment (FT) group, Fermentation fluid prevention (FP) group, ...

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Abstract

The invention relates to the field of fermentation engineering, in particular to a ganoderma lucidum sporocarp microbial fermentation preparation for treating intestinal flora disorder and intestinalbarrier function impairment. A ganoderma lucidum sporocarp extract and probiotic strains are fermented together to obtain a fermented product, ganoderma lucidum sporocarp powder is prepared into a ganoderma lucidum extract with the concentration of 5-35 mg / ml, and then the ganoderma lucidum extract with the concentration of 5-35 mg / ml is added into an MRS culture medium with the sugar content of 0.1-1%. Preferably, the inoculated probiotics are bacillus subtilis, bacillus subtilis strains are inoculated into the MRS culture medium containing the ganoderma lucidum extract at the concentration of 1%, anaerobic culture is conducted for 48 h in the environment of 37 DEG C after inoculation, fermentation is conducted, and a fermentation product is obtained, so that damage, caused by flora disturbance, to the intestinal barrier function can be relieved.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a ganoderma lucidum fruiting body microbial fermentation preparation for treating intestinal flora disorder and impaired intestinal barrier function. Background technique [0002] Ganoderma lucidum (scientific name: Ganoderma Lucidum Karst), which is umbrella-shaped in shape, with a kidney-shaped, semicircular or nearly round cap, is the fruiting body of Ganoderma lucidum, a polyporaceae fungus. Ganoderma lucidum has different degrees of curative effect on neurasthenia, inflammation, hyperlipidemia, coronary heart disease and angina pectoris. [0003] Fruiting body is the spore-forming structure of higher fungi, that is, the fruiting body, which is composed of organized mycelium and is the main edible and medicinal part of edible and medicinal fungi. Ganoderma lucidum fruiting body powder refers to the powder of Ganoderma lucidum fruiting body pulverized by ultra-fine cru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074A61K35/742A61P1/00
CPCA61K35/742A61K36/074A61K2236/19A61P1/00A61K2300/00
Inventor 孔振贾尚一李明刘赫袁杰力
Owner 合肥康春堂药业有限责任公司
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