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A method for inducing Trichochromospora cocoa to produce conidia rapidly

A technology of conidia and hair color, applied in the direction of using spores, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of unstable sporulation, shorten the time of sporulation, and improve the stability of sporulation performance, high application potential

Active Publication Date: 2021-11-09
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It usually takes one month or even longer to obtain the conidia of Trichoderma cocoa by using the above method, and the sporulation quantity is unstable, so it is impossible to obtain pathogenicity analysis and conidia infection process in a short period of time. et al. provide the large number of conidia suspensions required

Method used

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  • A method for inducing Trichochromospora cocoa to produce conidia rapidly
  • A method for inducing Trichochromospora cocoa to produce conidia rapidly
  • A method for inducing Trichochromospora cocoa to produce conidia rapidly

Examples

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preparation example Construction

[0028] The preparation method of the PDA medium described in the following examples is:

[0029] Peel and chop 200g of potatoes, cook in boiling water for 20min, filter out the residue, add 18g of agar powder and 20g of glucose, dissolve and set the volume to 1000mL, autoclave for 20min, and fill 20mL in each petri dish to obtain PDA medium.

Embodiment 1

[0030] Embodiment 1 A kind of method of inducing Cocoa Trichodisporium to produce conidia

[0031] A method for inducing Trichochromodiospora cocoa to produce conidia, comprising the steps of:

[0032] 1. Activation of Trichodiospora cocoa

[0033] Cocoa Trichochromodiaceae strain CSS-01s (Yan, J Y., Xie, Y., Zhang, W. et al. Species of Botryosphaeriaceae involved in grapevine dieback in China. Fungal Diversity. 2013, 61: 221-236.) was preserved in 4°C refrigerator, use a sterilized toothpick to take an appropriate amount of mycelium and put it on the PDA medium, seal the culture dish with parafilm, put it upside down and put it in a 28°C constant temperature incubator for activation for 3 days, and get activated cocoa hair color II Spores, for use.

[0034] 2. Preparation of sporulation medium

[0035] Take 20g of agar, add water to make up to 1000mL, and sterilize in an autoclave at 121°C for 20min to obtain a sterilized water agar medium;

[0036] Cut the green branches...

Embodiment 2

[0043] Embodiment 2 A kind of method of inducing Cocoa Trichoderma to produce conidia

[0044]A method for inducing P. cocoa to produce conidia. The difference from Example 1 is that in step 3, "seal the culture dish with parafilm and put it in a constant temperature incubator at 28°C, under 14h light and 10h dark conditions "Cultivate for 9 days" was replaced with "seal the culture dish with parafilm and put it in a constant temperature incubator at 28°C, and cultivate it for 11 days under 14h light and 10h dark conditions", and the others were exactly the same as in Example 1. The result is as Figure 5 shown.

[0045] After measurement, the cocoa hair color dispora sporulation yield that the present embodiment obtains is 1.28 * 10 5 piece / cm 2 , the "cm 2 ” refers to the surface area of ​​grape shoots exposed to the water agar medium.

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PUM

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Abstract

The invention discloses a method for inducing T. cocoa to rapidly produce conidia. The present invention firstly discloses the application of grape branches in inducing the production of conidia by Trichodina cocoa. The invention further discloses a method for inducing Trichodiosporium cocoa to produce conidia. In the present invention, the sporulation culture medium prepared by the water agar medium and grape branches is used to cultivate the cocoa hair color dispora, which greatly shortens the time for the cocoa hair color dispora to produce conidia, improves the sporulation stability and largely eliminates It avoids the pollution of other miscellaneous bacteria, and provides sufficient basic conditions for the research on the pathogenic mechanism, infection process, disease occurrence rules and control methods of Trichochromodiospora cocoa, and has high application potential.

Description

technical field [0001] The invention belongs to the technical field of phytopathogenic fungus induced sporulation, and in particular relates to a method for inducing the rapid production of conidia by Dispora cocoa. Background technique [0002] Lasiodiplodia theobromae is an important plant opportunistic fungus with a wide range of hosts. It can infect many perennial woody plants such as grapes, mangoes, papayas, peaches, etc. It can cause serious losses under suitable conditions. . The current research reports on Trichochromospora cocoa mainly focus on the isolation and identification of the pathogen, biological characteristics, and analysis of pathogenicity. Prevention and control provides a theoretical basis. Among them, obtaining conidia of pathogenic bacteria is a key link in the study of gene function and infection process. How to obtain a large number of conidia quickly and easily determines the research progress of pathogenic bacteria to a large extent. Under the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00C12N1/14C12R1/645
CPCC12N1/14C12N3/00
Inventor 李兴红韩昌坪张玮燕继晔刘梅彭军波邢启凯
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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