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Culture medium and preparation method for promoting spore production of Nomura rayii

A technology of Nomura spp. and culture medium, which is applied in the field of medium preparation to promote spore production of Nomura spp., can solve the problems of long culture period of medium, shorten the time of spore production and increase the amount of spore production

Inactive Publication Date: 2017-07-18
LIAOCHENG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The medium commonly used in the research of Nomura rayii is Sabouraud's maltose-yeast medium (SMAY), and the rate and amount of sporulation are better than those of Sabouraud's dextrose-yeast medium (SDAY) and potato dextrose medium (PDA). However, the medium still has a long culture period

Method used

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  • Culture medium and preparation method for promoting spore production of Nomura rayii
  • Culture medium and preparation method for promoting spore production of Nomura rayii
  • Culture medium and preparation method for promoting spore production of Nomura rayii

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Experimental program
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Effect test

Embodiment

[0019] Embodiment: A kind of culture medium preparation method that promotes the spore production of Nomura reinerii.

[0020] 1. Nomura culture medium is prepared from the following raw materials (1 liter medium):

[0021] Maltose 10 grams;

[0022] 10 grams of peptone;

[0023] 10 grams of yeast extract;

[0024] 12 grams of agar;

[0025] 40 grams of mature larvae of Spodoptera litura;

[0026] Two, the preparation method of Nomura culture medium:

[0027] (1) Accurately weigh 10 grams of maltose, 10 grams of peptone, 10 grams of yeast extract, and 12 grams of agar powder, mix them well and place them in a 500 ml beaker, then add 200 ml of distilled water and stir for 10 minutes with a magnetic stirrer until Each component dissolves evenly;

[0028] (2) Weigh 40 grams of frozen dead Spodoptera litura larvae (mature larvae are preferred), add 50 ml of distilled water in a mortar and grind until there is no obvious large tissue;

[0029] (3) Mix the homogenate of Spodo...

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Abstract

The invention aims to provide a culture medium for promoting spore production of nomuraea rileyi and a preparation method. According to the culture medium for promoting spore production of nomuraea rileyi and the preparation method, conidiospore of nomuraea rileyi can be obtained more rapidly and more effectively. According to the scheme, appropriate insect tissue components are added, the nutrition-allocated proportion is changed, stimulating factors for nomuraea rileyi growth are increased, and accordingly, the culture efficiency is improved.

Description

technical field [0001] The invention belongs to the field of biological control of agricultural diseases and insect pests, and in particular relates to a preparation method of a culture medium for promoting spore production of Nomura spp. Background technique [0002] The harm of pests to plants is one of the important factors causing agricultural production reduction. According to the estimates of the Food and Agriculture Organization of the United Nations (FAO), about 14% of the world's grain production is lost due to pests; the loss rate of grain in my country is between 10-15%, the loss rate of vegetables is 15%-25%, and the loss rate of fruits is 20%-30% %. It can be seen that the occurrence of insect pests is an important factor restricting the high yield of agriculture. In order to prevent or reduce the economic loss caused by insect feeding and prevent the harm of pests to agricultural products, farmers have to spray a large amount of chemical pesticides. The use of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00C12R1/645
Inventor 刘守柱徐广增杨淑仪陶新娉王彦管清真
Owner LIAOCHENG UNIV
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