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A kind of large-scale production fermentation medium and fermentation culture method of Ascospora platina

A technology of Ascospora platinospores and fermentation medium, which is applied in the field of large-scale production of Ascospora platinospores fermentation medium and fermentation culture, and can solve the problem of low sporulation efficiency and high cost of fermentation medium of Ascospora platinospores , production and application barriers, etc., to achieve the effects of shortening the production cycle, good application prospects, and low prices

Active Publication Date: 2017-03-29
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] As an important biological control factor, many studies have used the fermentation product of Ascospora platinospores for biological control. However, due to the high cost of the fermentation medium and the long fermentation period of the current Ascospora platinospores, it usually takes 28 days to achieve the highest spore production. Compared with the 14-15 day fermentation period of the widely used biocontrol fungus Beauveria bassiana, the spore production efficiency of Ascospora platinum is low and the cost is high, which hinders its production and application

Method used

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  • A kind of large-scale production fermentation medium and fermentation culture method of Ascospora platina
  • A kind of large-scale production fermentation medium and fermentation culture method of Ascospora platina

Examples

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Embodiment 1

[0018] Screening of Example 1 Strain

[0019] 1.1 Source of material

[0020] In my country, Hubei, Hunan, Jiangxi, Zhejiang, Sichuan, Chongqing, Guizhou, Fujian, Guangxi, Guangdong and other major citrus producing areas, collected citrus whitefly carcasses infected by Conchosporium platysma.

[0021] Potato dextrose agar medium (PDA): boil 200g of potatoes to extract juice, add 20g of glucose and 20g of agar powder, add water and make up to 1000mL and boil. Then sterilize in a sterilizer (121°C, 30min).

[0022] Aseptic operation conditions: All utensils and utensils must be sterilized in an autoclave (121°C, 30min), and operations such as inoculation are carried out in a clean bench.

[0023] Cultivation conditions: culture in an incubator with a temperature of 25°C and light (16L:8D), after the colonies are formed, transfer to a PDA slope, and then transfer to a 4°C refrigerator for storage.

[0024] 1.2 Isolation and identification of pathogenic bacteria

[0025] 1.2.1...

Embodiment 2

[0047] The invention relates to a fermentation medium for Conchospora platina, which is composed of the following components by weight: 20% of peanut cake flour, 0.1% of glucose, 0.1% of tryptone, and water as the balance.

[0048] The above-mentioned Conchospora platysma fermentation medium can be prepared according to conventional methods, and the sterilization conditions are: 121° C., pressure range 0.1MPa-0.15MPa, and time 30 minutes.

[0049] Fermentation culture method of Conchosporium platysma: take the S. platina sp. YW3 strain with the preservation number of CCTCC NO: M2013448, inoculate it on the PDA medium, place it in a constant temperature incubator at 25 ± 1 °C for 3 weeks, and use containing Spores were collected in 0.05% Tween-80 in water and formulated into 5 x 10 5 spore / ml suspension, finally inoculate the suspension into the above fermentation medium, the volume of the suspension is 10% of the volume of the fermentation medium, after inoculation, place it a...

Embodiment 3

[0051] The invention relates to a fermentation medium for Concetrium platysma, which is composed of the following components by weight: 25% of peanut cake flour, 0.05% of glucose, 0.05% of tryptone, and water as the balance.

[0052] Fermentation culture method of Conchosporium platysma: take the WD3 strain of Conchosporium platysma with the preservation number of CCTCC NO: M2013447, inoculate it on the PDA medium, place it in a constant temperature incubator at 25 ± 1 °C for 2 weeks, and use the Spores were collected in 0.05% Tween-80 in water and formulated into 1 x 10 5 A suspension of spores / ml, inoculate the suspension into the above fermentation medium, the volume of the suspension is 15% of the volume of the fermentation medium, and culture at 27° C. for 15-16 days after inoculation.

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Abstract

The invention discloses a low-cost culture medium for large-scale production and fermentation of Ascospora floridae, which consists of the following components: 15-25% of peanut cake powder, 0.05-0.2% of glucose, 0.05-0.2% of tryptone, and the remainder of water quantity. The invention also discloses a method for fermenting and cultivating Aschersonia placenta. The method adopts three Aschersonia placenta strains YW3, WD3 and ZJ5 with high pathogenicity and high spore-producing ability, which are preserved in China. Typical Culture Collection Center, the collection numbers are CCTCC NO: M2013448, CCTCC NO: M2013447, CCTCC NO: M2013446. The fermenting and cultivating method provided by the present invention obtains a high-pathogenicity and high-content Ascospora spp., which has good application prospects for the control of whitefly pests.

Description

technical field [0001] The invention relates to a large-scale production fermentation medium and a large-scale production fermentation culture method of Aschersonia placenta. Background technique [0002] Concetrium platysma is an important pathogenic fungus for whitefly pests. It has the characteristics of strong pathogenicity to whitefly pests, non-toxic to humans, animals and plants, does not kill natural enemies, and does not pollute the environment. More pests biological control of pathogenic fungi, and successfully used in the biological control of whiteflies and scale insects in tea orchards. Investigations show that Concetrium platysma can form an epidemic in whitefly populations and has a natural control effect. as high as 90% or more. [0003] As an important biological control factor, many studies have used the fermentation products of Conceta platysma for biological control. However, due to the high cost of the fermentation medium and the long fermentation cycl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14C12N1/145C12R2001/645
Inventor 张宏宇张为丽
Owner HUAZHONG AGRI UNIV
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