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Application of peroxyergoterol in agaricus gennadii in CDC25 phosphoprotease

A technology of peroxyergosterol and phosphoprotease, which is applied in the directions of medical preparations, steroids, and pharmaceutical formulations containing active ingredients, can solve problems such as low compound content, and achieves high product yield, low production cost, and convenience. separation effect

Inactive Publication Date: 2020-02-28
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Peroxyergosterol widely exists in edible and medicinal fungi, but the content of this compound is very low, and it is still very difficult to directly extract peroxyergosterol from fungi to obtain sufficient raw materials

Method used

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  • Application of peroxyergoterol in agaricus gennadii in CDC25 phosphoprotease
  • Application of peroxyergoterol in agaricus gennadii in CDC25 phosphoprotease
  • Application of peroxyergoterol in agaricus gennadii in CDC25 phosphoprotease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of the crude extract of each active component in the mushroom tortuosporum of embodiment 1

[0035] Take 10kg of fresh, undamaged Agaricus torusum fruiting body, slice it and soak it with 30L 95% ethanol for 7 days (wherein the ratio of Agaricus toricosporus fruiting body to ethanol is 1:3, so that the ethanol can completely soak the Agaricus toricosporin fruiting body), filter The filtrate was obtained, the solvent was recovered under reduced pressure, and 30 L of 95% ethanol was added to the filter residue for ultrasonic extraction 3 times, each time for 2 hours. Concentrate under reduced pressure to obtain 95% ethanol extract. Operate in the same way with 65% ethanol, and concentrate under reduced pressure to obtain 65% ethanol extract. Add 30L of distilled water to the filter residue of the mushroom after alcohol extraction, and extract three times with 1800HZ ultrasound, each time for 2 hours. Concentrate under reduced pressure to obtain 538 g of wate...

Embodiment 2

[0040] Example 2 Petroleum ether phase component separation and purification

[0041] 10.0 g of the petroleum ether fraction obtained in the above example 1 was separated by medium-pressure silica gel column flash chromatography, petroleum ether-ethyl acetate system (100:0-0:100) gradient elution, and the eluent was detected by thin-layer chromatography. The similar fractions were combined, and the solvent was recovered to obtain a total of 9 components A to I. Component D is separated by medium-pressure silica gel chromatography to obtain D1 and D2. D1 was recrystallized to obtain 20 mg of compound 3 in the form of needles. D2 was separated by multiple medium-pressure silica gel chromatography and Sephadex LH-20 column chromatography to obtain 10 mg of compound 2 as a white solid. Component E was separated by multiple medium-pressure silica gel chromatography and Sephadex LH-20 column chromatography to obtain 5 mg of compound 1 as a yellow-green oily solid.

[0042] Among ...

Embodiment 3

[0054] Example 3 Separation and Purification of Ethyl Acetate Part Components

[0055] 6.0 g of the ethyl acetate fraction obtained in the above-mentioned Example 1 was reversed phase C by medium pressure 18 Column flash chromatography separation, water-methanol solvent system (5% to 100%) gradient elution, the eluents are combined according to the same gradient, and the solvent is recovered to obtain 6 components from I to VI. After checking by high performance liquid chromatography, it was found that components I to IV were common components in the n-butanol phase, so they were merged into the n-butanol phase. Ⅴ component through medium pressure reverse phase C 18 After detaching, use C again 18 After semi-preparative column separation, a total of 4 mg of compound 4, a total of 4 mg of compound 5 and a total of 3 mg of compound 24 were obtained. Compound 4 is a yellow granular solid; Compound 24 is a yellow granular solid; Compound 5 is a red granular solid.

[0056] whe...

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Abstract

The invention discloses application of peroxyergoterol which is an active ingredient in agaricus gennadii. Active ingredients of agaricus gennadii are quickly separated through a separation and extraction method, and a separation and extraction process is simple, stable, suitable for industrial continuous production, high in product yield and low in production cost; peroxyergoterol in the active ingredients of agaricus gennadii has effect of inhibiting activity of CDC25 phosphoprotease, thereby being supportive of being applied to diseases related to CDC25 phosphoprotease.

Description

Technical field: [0001] The present invention relates to the technical field of application of natural products, a kind of extracting active ingredient peroxyergosterol from natural products and its application, more specifically relates to extracting peroxyergosterol from mushroom tortuosporus and its peroxyergosterol on CDC25 phosphoprotease Applications. Background technique: [0002] Mushroom family (Agaricaceae) is a family of fungi Agaricaceae, with many types and wide distribution. Most of them are edible, and they are distributed all over the world. The mushroom family has 28 genera including the genus Agaricus. Agaricus gennadii (Chot.et Boud) P.D.Orton, Agaricus bisporus (Large) Sing., Agaricus blazei murrill, Agaricus rubellus (Gill.) Sacc., brown mushroom Agaricus crocopelusspeck, etc. At present, researches on the chemical constituents and pharmacological activities of this genus are mainly focused on Agaricus bisporus and Agaricus blazei. [0003] The resea...

Claims

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Application Information

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IPC IPC(8): A61K31/58A61P35/00C07J71/00
CPCA61K31/58A61P35/00C07J71/0005
Inventor 吴疆廖志明林鹏程
Owner QINGHAI UNIV FOR NATITIES
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