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A corn stalk degrading composite bacterial agent

A technology of composite bacterial agent and corn stalks, which is applied in the direction of bacteria, fungi, microorganisms, etc., can solve the problems of straw degradation effect discount and achieve high stability and high degradation rate

Active Publication Date: 2021-11-02
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microorganisms in the environment have the characteristics of fast proliferation, rich and diverse functions, and strong adaptability. The application of microbiological methods to decompose straw has many irreplaceable advantages of other physical and chemical methods, and has broad application prospects. Therefore, from natural screening to efficient Strains with straw degrading ability are very important. There are many straw decomposing agents in China, but most of them have a greatly reduced effect on straw degradation due to the limitation of ambient temperature or bacterial growth rate.

Method used

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  • A corn stalk degrading composite bacterial agent

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Experimental program
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Effect test

Embodiment 1

[0025] The mutagenic screening of embodiment 1 verrucous Myromyces

[0026] (1) Morphological identification and screening

[0027] The soil in the forest area of ​​Changbai Mountain Nature Reserve was collected and cultivated on PDA medium by conventional methods. The mycelium of the strain was white flocculent at first, and grew divergently towards the periphery. After 8 days of culture, the color of conidia continued to deepen, and glue dots appeared; after 10 days of culture, the colony was in the shape of concentric rings, the conidia showed black glue spots, and the back of the colony appeared Hazel radial folds.

[0028] Use a puncher to get a 1cm-diameter bacterial block, inoculate it on an aniline blue selective medium, and culture it in an aerobic incubator at 30°C for 10 days, observe the fading of aniline blue, and select a strain with a fast fading rate and strong ability as candidate strains.

[0029] At the same time, in order to directional screen lignin-deg...

Embodiment 2

[0035] Embodiment 2 The screening of the mutant Aspergillus niger strain that produces β-glucosidase obtains

[0036] Randomly sample soil, add 1g soil sample into 10mL sterile water, shake well, draw 1mL into liquid PDA medium (200g / L potato, 20g / L glucose, 15g / L agar), shake at 180r / min at 28°C Bed culture for 24h. After the strain was enriched and cultivated, it was diluted 10 4 -10 7 times, each 50 μl dilution was spread on PDA medium, cultured at 28°C for 3 days, and a single colony was picked for primary screening of strains.

[0037] Preliminary screening: Aspergillus niger producing β-glucosidase strain in the presence of P-NPG (p-nitrophenyl-β-D-glucopyranoside 20g / L, yeast powder 10g / L, peptone 20g / L, agar 20g / L L) is the screening medium with the sole carbon source, and the strain with obvious yellow halo is the target strain. The beta-glucosidase-producing strain is isolated and inoculated in a liquid fermentation medium for screening high-beta-glucosidase-prod...

Embodiment 3

[0041] Example 3 Preparation of Corn Stalk Degradation Composite Bacteria Agent

[0042] A corn stalk-degrading composite bacterial agent, the active ingredient of which is the following raw materials in mass percentage: 20% of cellulase-producing Paenibacillus, 15% of xylanase-producing Alternaria, laccase-producing wart 20% Mycobacterium sporogenes, 10% lignin peroxidase-producing Bacillus colioids, 10% manganese peroxidase-producing Layseia cerevisiae, 5% Saccharomyces cerevisiae, 5% lactic acid bacteria, parasmooth candida Yeast 5%, β-glucosidase-producing mutant Aspergillus niger 5%, Bacillus amyloliquefaciens 5%, among the above bacterial agents:

[0043] The cellulase-producing Paenibacillus (Bacillus cellulosilyticus) CGMCC 1.15312;

[0044] The xylanase-producing Alternaria humicola CGMCC 3.2917;

[0045] The lignin peroxidase-producing colloid bacillus (Bacillus mucilaginosus Krassilnikov) GIM 1.16;

[0046] The manganese peroxidase-producing Actinomycetes (Laceye...

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Abstract

The invention provides a corn stalk-degrading composite bacterial agent. The 10 bacterial strains of the invention are mutually beneficial and symbiotic, synergistic, and have no antagonism. They can degrade corn stalks at 10°C, and the decomposing time is advanced to 5-6 days. When the composite bacterial strain of the present invention is cultivated to 26 generations, there is no obvious difference in the degradation rate of corn stalks at 10° C. among different generations, and it has high stability.

Description

technical field [0001] The invention relates to a corn stalk degrading composite bacterial agent, which belongs to the intensive agricultural production technology, and is specially used for degrading waste corn stalks to produce organic fertilizers and realizing resource utilization of organic waste. Background technique [0002] Corn has been cultivated in my country for more than 470 years. Due to its high yield, good quality and strong adaptability, the cultivated area has developed rapidly. At present, my country's sown area is about 300 million mu, second only to rice and wheat, and ranks third among food crops, second only to the United States in the world. It is an important food crop and fodder crop, and is also the crop with the highest total output in the world. . Corn has always been known as a longevity food, rich in protein, fat, vitamins, trace elements, cellulose, etc., and has great potential for developing high-nutrition, high-biological function foods. Co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C12R1/07C12R1/01C12R1/865C12R1/72C12R1/645C12R1/685
CPCC12N1/14C12N1/16C12N1/20
Inventor 陈光孙旸张斯童王刚常浩苏瑛杰于潇潇
Owner JILIN AGRICULTURAL UNIV
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