Artificial ligament having immunization bone formation function and preparation method of artificial ligament
An artificial ligament and functional technology, applied in the medical field, can solve problems such as low biomechanical properties
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Embodiment 1
[0025] Such as figure 1 Shown, a kind of artificial ligament preparation method with immune osteogenesis function, comprises steps:
[0026] (1) Put the PET artificial ligament of certain size in the ultrasonic machine, carry out ultrasonic cleaning to this PET artificial ligament with 75% ethanol, the time is 25min; Then use deionized water to clean described PET artificial ligament; Finally put The PET artificial ligament was naturally dried at room temperature for 24 hours;
[0027] (2) Cut the PET artificial ligament obtained in step (1), put the cut PET artificial ligament into a 100mL beaker, then add 25mL of Tris-HCL solution with a pH of 8.5 into the beaker, and stir with a small rotor Mix the PET artificial ligament with the Tris-HCL solution fully; then add 25 mL of dopamine hydrochloride solution with a concentration of 4 mg / mL into the beaker, and react for 1 hour; then take out the PET artificial ligament and wash off the excess surface of the PET artificial liga...
Embodiment 2
[0031] Such as figure 1 Shown, a kind of artificial ligament preparation method with immune osteogenesis function, comprises steps:
[0032] (1) Put the PET artificial ligament of certain size in the ultrasonic machine, carry out ultrasonic cleaning to this PET artificial ligament with 75% ethanol, the time is 25min; Then use deionized water to clean described PET artificial ligament; Finally put The PET artificial ligament was naturally dried at room temperature for 24 hours;
[0033] (2) Cut the PET artificial ligament obtained in step (1), put the cut PET artificial ligament into a 100mL beaker, then add 25mL of Tris-HCL solution with a pH of 8.7 into the beaker, and stir with a small rotor Mix the PET artificial ligament with the Tris-HCL solution fully; then add 25 mL of dopamine hydrochloride solution with a concentration of 4 mg / mL into the beaker, and react for 12 hours; then take out the PET artificial ligament and wash off the excess surface of the PET artificial li...
Embodiment 3
[0037] Such as figure 1 Shown, a kind of artificial ligament preparation method with immune osteogenesis function, comprises steps:
[0038] (1) Put the PET artificial ligament of certain size in the ultrasonic machine, carry out ultrasonic cleaning to this PET artificial ligament with 75% ethanol, the time is 25min; Then use deionized water to clean described PET artificial ligament; Finally put The PET artificial ligament was naturally dried at room temperature for 24 hours;
[0039] (2) Cut the PET artificial ligament obtained in step (1), put the cut PET artificial ligament into a 100mL beaker, then add 25mL of Tris-HCL solution with a pH of 9.0 into the beaker, and stir with a small rotor Mix the PET artificial ligament with the Tris-HCL solution fully; then add 25 mL of dopamine hydrochloride solution with a concentration of 4 mg / mL into the beaker, and react for 24 hours; then take out the PET artificial ligament and wash off the excess surface of the PET artificial li...
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