Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of dimethyl itaconate in prevention and treatment of ulcerative colitis and canceration of ulcerative colitis

A technology for dimethyl itaconate and ulcerative colitis is applied in the application field of dimethyl itaconate in the prevention and treatment of ulcerative colitis and its carcinogenesis, so as to reduce the risk of colon cancer and prevent ulcerative colitis. effects of colitis

Active Publication Date: 2020-01-31
SUN YAT SEN UNIV +1
View PDF3 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the use of dimethyl itaconate (DI) for the prevention and treatment of ulcerative colitis and its canceration

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of dimethyl itaconate in prevention and treatment of ulcerative colitis and canceration of ulcerative colitis
  • Application of dimethyl itaconate in prevention and treatment of ulcerative colitis and canceration of ulcerative colitis
  • Application of dimethyl itaconate in prevention and treatment of ulcerative colitis and canceration of ulcerative colitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] DI effectively inhibits DSS-induced ulcerative colitis

[0044] 1. The test method is as follows:

[0045] (1) The 8-week-old mice were randomly divided into two groups, the ears of the mice were punched and marked, and the body weight of the mice was recorded once a day.

[0046] (2) After the mice were observed and stabilized in the animal room for one week, they drank water with 3% DSS solution for 5 days, and then drank normal water for 5 days.

[0047] (3) While administering 3% DSS solution in drinking water, one group was injected with 200 μL PBS (containing 20 mg DI), and the other group was injected with 200 μL PBS, and no administration was given during normal drinking water.

[0048] (4) After 10 days, observe the stool properties or blood in the stool, and terminate the experiment.

[0049] (5) After the mice were killed by cervical dislocation, the colorectum was separated and removed, and pathological staining, QPCR detection, and Western blotting were per...

Embodiment 2DI

[0058] Example 2 DI can reduce the risk of developing colon cancer from ulcerative colorectum

[0059] 1. The test method is as follows:

[0060] (1) The mice were randomly divided into two groups, the ears of the mice were punched and marked, and the body weight of the mice was recorded every five days.

[0061] (2) After the mice were observed and stabilized in the animal room for one week, they were intraperitoneally injected with a dose of 10 mg / kg.

[0062] (3) After 5 days of injection, the mice were continuously given 2% DSS solution drinking water for 5 days, and then given normal drinking water for 16 days, a total of 3 cycles.

[0063] (4) After the three cycles, observe the stool properties or blood in the stool, and observe whether there is tumor prolapse in the anus of the mouse. If there is an obvious tumor in the anus of the mouse or the test period reaches 80 days, the experiment can be terminated.

[0064] (5) After the mice were successfully anesthetized, t...

Embodiment 3

[0072] Example 3 DI can reduce the secretion of IL-1β and CCL2

[0073] 1. The test method is as follows:

[0074] (1) Before the experiment, put the detection kit at room temperature to balance for 20 minutes, and all liquid components should be shaken well before use. Frozen serum was thawed on ice.

[0075] (2) Take out the required strips from the aluminum foil bag, and seal the remaining strips in a ziplock bag and store them at 4°C until use.

[0076] (3) Set up 6 protein standard products, the standard product is 2000pg / ml, and the concentrations of protein standard products are sequentially diluted to 500pg / ml, 250pg / ml, 125pg / ml, 62.5pg / ml, 31.25pg / ml, 0pg / ml ml, each concentration of 100 μl sample was added to the well plate.

[0077] (4) After 5-fold dilution of the sample, 100 μl was added to the well plate, covered with a plate sticker, and incubated at 37°C for 2 hours.

[0078] (5) Discard the liquid, shake dry, no need to wash.

[0079] (6) After diluting ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of dimethyl itaconate in prevention and treatment of ulcerative colitis and canceration of the ulcerative colitis. Specific tests prove that the dimethyl itaconate can effectively prevent the ulcerative colitis and reduce the risk of development of colon cancer by the ulcerative colorectal, and provides novel ideas for the prevention and treatment of the ulcerative colitis and the canceration of the ulcerative colitis.

Description

technical field [0001] The invention relates to the application of dimethyl itaconate in the prevention and treatment of ulcerative colitis and its canceration. Background technique [0002] Colorectal cancer (CRC) is the third most common malignant tumor and the fourth most lethal tumor in the world. Inflammatory bowel disease (IBD), a long-term relapsing inflammatory disease, is a high risk factor for colorectal cancer. Accumulating evidence now supports the notion that inflammation is an important component of tumor progression. Many cancers are caused by infection, chronic irritation and inflammation. [0003] Tumor-associated macrophages (TAMs) are an important part of the inflammatory infiltration of tumor tissue, mainly recruited by monocytes by chemoattractant protein (MCP) chemokines. A number of clinical studies have shown that in patients with ulcerative colitis and colorectal cancer, the number of macrophages in colorectal tissues is significantly higher than ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/22A61P1/00A61P1/04A61P35/00
CPCA61K31/22A61P1/00A61P1/04A61P35/00Y02A50/30
Inventor 曾木圣冯国开王茜叶嘉聪梅琰李新玲
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com