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Method for detecting liver and kidney clearance rates of two isomers of Primovist

A detection method, a technology of renal clearance rate, which is applied in the field of biochemical analysis, can solve the problems of slow net conversion speed, etc., and achieve the effect of simple operation and high accuracy

Active Publication Date: 2020-01-17
中国人民解放军总医院第八医学中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to previous research and our experimental verification, such a net conversion rate in the human body is very slow, so slow that it can be ignored

Method used

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  • Method for detecting liver and kidney clearance rates of two isomers of Primovist
  • Method for detecting liver and kidney clearance rates of two isomers of Primovist
  • Method for detecting liver and kidney clearance rates of two isomers of Primovist

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 double blood plasma method measures the plasma clearance rate of Gd-A and Gd-B

[0041] The study was approved by the review board and written informed consent was obtained from all patients. Between July 2017 and February 2018, we conducted the study and performed data analysis on 41 patients (mean age 41.8±12.1 years, range 30-73 years). Of these, 33 had diffuse cirrhosis, 29 had hepatitis B cirrhosis, and 4 had alcoholic cirrhosis. Among the 33 patients with diffuse liver cirrhosis, 19 cases had a diameter larger than 2 cm; among the 33 patients with diffuse liver disease, 18 cases were classified as Child-Pugh classification A, and 15 cases were classified as B. In addition, 8 observers without cirrhosis, each patient had at least one liver nodule or mass larger than 1 cm in diameter.

[0042] For each patient, 1 ml of iodine contrast medium (iopromide, 370 mg iodine per ml; Bayer Schering, Berlin, Germany) was injected intravenously followed by 20 ml...

Embodiment 2

[0061] The renal clearance rate detection of embodiment 2Gd-A and Gd-B

[0062] During the detection period in Example 1, the patient's urine was collected between 1 h and 3 h after the injection of Gd-EOB-DTPA, and the urine sample was subjected to HPLC analysis. The conditions of the HPLC analysis were the same as in Example 1. The concentration of Gd-A and Gd-B in the urine was measured and multiplied by the volume of the urine to calculate the amount of Gd-A and Gd-B excreted through urine during the double plasma sampling period of Example 1.

[0063] Use Excel to draw a graph, the horizontal axis is the time of blood sample collection, and the vertical axis is the plasma concentration of Gd-A and Gd-B determined by the double plasma method. For each isomer, an exponential curve connecting the two points (1h and 3h) in the coordinates was obtained using Excel's curve fitting function by integrating the area under the curve (AUC) and dividing the urine volume by the AUC, ...

Embodiment 3

[0067] The hepatic clearance calculation of embodiment 3Gd-A and Gd-B

[0068] In this example, the hepatic clearance rate of Gd-A (HCL-GdA) and the hepatic clearance rate of Gd-B (HCL-GdB) were calculated. Calculated as follows:

[0069] RCL-GdA—GFR=HCL-GdA;

[0070] RCL-GdB—GFR=HCL-GdB, after calculation, the calculation results of Gd-A and Gd-B hepatic clearance in 41 patients are shown in Table 4.

[0071] Table 4

[0072]

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Abstract

The invention relates to a method for detecting liver and kidney clearance rates of two diastereoisomers Gd-A and Gd-B of Primovist. The method is characterized in that during regular dose administration of Primovist ( for example, intravenous administration during magnetic resonance enhanced imaging), a low dose of iodinated contrast agent (about 1% of the conventional dose) is given at the sametime, a small amount of blood sample is extracted to obtain the plasma clearance rates of the iodinated contrast agent, the Gd-A and the Gd-B, the plasma clearance rate of the iodinated contrast agentis GFR, it is discovered in the method provided by the invention for the first time that the GFR can approximately replace the kidney clearance rates of the Gd-A and the Gd-B, and the liver clearancerates of the Gd-A and the Gd-B can be obtained by subtracting the GRF from the respective plasma clearance rates. By adopting the detection method provided by the invention, the liver and kidney clearance rates of the two isomers of Gd-A and Gd-B of the Primovist can be measured conveniently and accurately, a "gold standard" and individualized pharmacokinetic indicators are provided for the magnetic resonance liver function imaging of the Primovist, and the method plays guidance and reference roles for rational drug use and evaluation of liver and kidney functions.

Description

technical field [0001] The invention relates to the technical field of biochemical analysis, in particular to a method for detecting the liver and kidney clearance rates of two isomers of Promigen. Background technique [0002] Gadoxetic acid disodium (Gd-EOB-DTPA), trade name Primovist, international nonproprietary name (INN) is Gadoxetic acid disodium, is a liver cell-specific magnetic resonance contrast agent, entered clinical practice in 1992 It was first approved for use in clinical trials in Sweden in 2004, officially launched in China in 2011, and has been approved in more than 40 countries. It is formed by adding a fat-soluble ethoxybenzyl (EOB) to the gadolinium-pentetic acid meglumine (Gd-DTPA) molecule, which makes it both a non-specific extracellular contrast agent and a hepatobiliary specificity. Properties of sexual contrast agents. The half-life of Gd-EOB-DTPA is about 56 minutes, allowing MR imaging in the hepatobiliary phase or hepatocyte-specific phase wi...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/5008G01N33/5088
Inventor 袁小东彭阳红施兵
Owner 中国人民解放军总医院第八医学中心
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