Bifidobacterium animal and application thereof in controlling diabetes or hyperlipidemia, particularly in weight gain or obesity

A technology of animal bifidobacteria and bacteria, applied in the field of microorganisms, can solve the problems of fermentation agent monopoly, less application, and unexplained mechanism of action, so as to enhance functionality, relieve insulin resistance and leptin resistance, and improve body sugar. The effect of tolerance level

Active Publication Date: 2019-12-24
BEIJING BEINONG HONGZE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] From the perspective of functional food, probiotics have been proven to have a certain improvement effect, but there are few applications at present, and the mechanism of action has not been clearly explained, and many functional probiotic strains, fermentation agents and key technologies have been imported by foreign countries. monopoly
Studies have also shown that foreign probiotics are not necessarily very suitable for the physiological characteristics and physique of Chinese people

Method used

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  • Bifidobacterium animal and application thereof in controlling diabetes or hyperlipidemia, particularly in weight gain or obesity
  • Bifidobacterium animal and application thereof in controlling diabetes or hyperlipidemia, particularly in weight gain or obesity
  • Bifidobacterium animal and application thereof in controlling diabetes or hyperlipidemia, particularly in weight gain or obesity

Examples

Experimental program
Comparison scheme
Effect test

preparation example

[0068] Bacteria preparation

[0069] 1. Inoculate the activated 2-generation Bifidobacterium animalis A12 into the improved MRS liquid medium, and culture it anaerobically at 37°C for 12 hours. After the cultivation, centrifuge the culture system at 8,000g at 4°C for 10 minutes to collect the supernatant. Filter through a 0.22 μm filter membrane, adjust the pH to 6.5, and obtain the fermentation supernatant.

[0070] 2. Inoculate the 2-generation activated Bifidobacterium animalis A12 and Bifidobacterium animalis BB12 into the modified MRS liquid medium, culture anaerobically at 37°C for 12 hours, and centrifuge the culture system at 8,000g at 4°C for 10 minutes to collect the bacteria body. Wash 3 times with sterile PBS buffer (pH 7.4), resuspend in PBS solution, adjust the number of cells to 10 9 / mL to obtain A12 and BB12 live bacterial suspensions.

Embodiment 1

[0072] This example is used to illustrate the establishment of the Transwell cell model and the inhibitory effect of the fermentation supernatant of Bifidobacterium animalis A12 on glucose transport

[0073] 1. Inoculate Caco-2 cells on a 12-well Transwell chamber (membrane pore size 0.4 μm, growth area 1.12 cm 2 ), add 2×10 5 Each cell / mL suspension was 500 μL, and 1.5 mL of cell culture medium was added to the basal side (lower chamber, BL side), and the medium was changed once a day. When the cells grow and differentiate for up to 21 days, the transmembrane electrical resistance (TEER) exceeds 500Ω·cm 2 When , cell fusion is considered complete, and the Transwell cell model can be used for transport experiments.

[0074] The result is as figure 1 shown. TEER increased rapidly in the first 11 days and reached a maximum on the 12th day (1300±110.82Ω cm 2 ), indicating that Caco-2 cells had formed a dense monolayer structure. 12-21 days after cell inoculation, TEER incre...

Embodiment 2

[0081] This example is used to illustrate the effect of in vitro α-glucosidase and simulated digestive tract of animal bifidobacteria

[0082] 1) Preparation of cell contents

[0083] The strains (A12 and BB12) were inoculated in the improved MRS broth with an inoculum size of 2% by volume, cultured anaerobically at 37°C for 12 hours, and passed to three generations for future use. Centrifuge the activated bacterial liquid at 6 000×g at 4°C for 10 min, collect the supernatant, adjust the pH to 6.0, and filter the filtrate through a 0.22 μm filter membrane to obtain the fermentation supernatant; buffer the bacterial cell precipitation with sterile PBS solution (pH7.4), washed 3 times, resuspended in PBS solution, and adjusted the concentration of bacteria to about 1×10 9 CFU / mL, and after crushing with a beadbeater for 30 seconds, centrifuge at 12 000×g for 10 minutes, and collect the supernatant as the cell content.

[0084] 2) Determination of α-glucosidase

[0085] In a 2...

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Abstract

The invention relates to the field of microorganisms, in particular to a bifidobacterium animal and an application thereof in controlling diabetes or hyperlipidemia, particularly in weight gain or obesity. The preservation number of the bifidobacterium animal is CGMCC No.17308. The invention also discloses a product composition. The product composition contains thallus substances and / or metabolites of the bifidobacterium animal. The bifidobacterium animal provided by the invention is suitable for specific physiological physique of Chinese people, and has relatively high advantages in activityinhibition of alpha-glucosidase, transport inhibition of glucose, improvement of glucose tolerance level of organisms, relief of insulin resistance and leptin resistance, improvement of the secretionlevel of glucagon-like peptide-1, increase of islet cell size and islet beta cell number, and the like, so that development of diabetes or hyperlipidemia can be better controlled, and particularly weight gain and obesity of organisms can be controlled.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to an animal bifidobacterium strain and its application in controlling diabetes or hyperlipidemia, especially weight gain or obesity. Background technique [0002] With the development of the economy and the improvement of people's living standards, obesity, insulin resistance and impaired glucose tolerance caused by high-calorie and unreasonable diet (high fat, high protein, low carbohydrate) have caused people's Attention and research, and long-term bad eating habits will eventually lead to diabetes. In recent years, diabetes has become prevalent all over the world, and has become the third chronic disease that seriously endangers human health after tumors and cardiovascular diseases. my country is second only to India and has the second largest number of diabetic patients in the world. Therefore, the development of blood sugar The application of relevant preventive measures at this ...

Claims

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Application Information

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IPC IPC(8): A61K35/745A61P3/10A61P5/50A61P3/06A61P3/04A61P9/10A61P1/16A23L33/135
CPCA61K35/745A61P3/10A61P5/50A61P3/06A61P3/04A61P9/10A61P1/16A23L33/135A23V2002/00A23V2400/515A23V2200/3262A23V2200/328A23V2200/3204
Inventor 金君华李桐杨建珺张红星谢远红刘慧
Owner BEIJING BEINONG HONGZE BIOTECH CO LTD
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